{"id":2066,"date":"2020-05-20T00:35:22","date_gmt":"2020-05-20T00:35:22","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/?page_id=2066"},"modified":"2023-01-26T13:39:45","modified_gmt":"2023-01-26T13:39:45","slug":"lab-unit-1-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/laboratory-units\/lab-unit-1-online\/","title":{"rendered":"Lab Unit 1 &#8211; In Person Lab"},"content":{"rendered":"\n<header class=\"entry-header\">\n<h1 class=\"entry-title\" style=\"text-align: left\"><span style=\"color: inherit;font-family: inherit;font-size: 30px\"><span style=\"color: #000000\">Basic Laboratory Skills<\/span><\/span><\/h1>\n<p><span style=\"font-size: 14pt\"><strong>Scroll below for In-Person Labs<\/strong><\/span><\/p>\n<\/header>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/laboratory-units\/lab-unit-1-online-synchronous\/\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n\n\n<h2 class=\"wp-block-heading\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-269 size-large alignnone\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/lenses_large-1024x768.jpg\" alt=\"microscope objective lenses\" width=\"640\" height=\"480\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/lenses_large-1024x768.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/lenses_large-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/lenses_large-768x576.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/lenses_large.jpg 1792w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/h2>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"font-size: 14pt\"><span style=\"color: #000000\"><strong>General Lab Information<\/strong> &#8211; All lab units will be structured with Pre-Lab, In-Lab, and Post-Lab sections. You will work through some activities on your own before lab (Pre-Lab), other activities will be worked on as a group during your lab time (In-Lab), and there will generally be follow-up Post-Lab work.&nbsp; Be sure to check the <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/lab-schedule-assignments-spring-2022\/\" target=\"_blank\" rel=\"noopener noreferrer\">Lab Schedule<\/a> EACH WEEK before and after Lab.&nbsp; As you work through activities, you should answer all questions in your lab manual.&nbsp;&nbsp;<\/span><\/span><\/h4>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"font-size: 14pt\"><span style=\"font-weight: 400\"><span style=\"color: #000000\">We plan to hold all our BIO 181 labs in person for the semester.&nbsp; If the University changes to ONLINE classes, your TA will contact you and the In-Lab portion of lab will be held as a Zoom lab session during your regularly scheduled lab section meeting time.&nbsp; Your TA may also hold office hours via Zoom (and\/or in person).&nbsp; Please access the link below to set up the Zoom client on your computer.&nbsp; You will need to authenticate to be able to access the Zoom sessions. All of the instructions to get started and for authenticating are available at this site:<\/span><br><a href=\"https:\/\/ncsu.service-now.com\/delta?id=kb_article_ml&amp;sys_id=1bd4ff7cdbab6bc42fa0791c8c961991\" target=\"_blank\" rel=\"noopener noreferrer\">Getting Started with Zoom<\/a><\/span><\/span><\/h4>\n\n\n\n<div style=\"height:34px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-white-color has-vivid-red-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2022\/08\/Lab1_PreLab_handoutBL.docx\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click to get Pre-Lab Assignment<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<h3 class=\"entry-content wp-block-heading\"><span style=\"color: #000000\">Complete the following and submit on Moodle before you come to lab:&nbsp;<\/span> <\/h3>\n\n\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Introduction to Lab 1:<\/span><\/h3>\n\n\n\n<ul class=\"has-vivid-cyan-blue-color has-text-color wp-block-list\"><li><span style=\"color: #000000;font-size: 12pt\">Read the following: Getting Oriented (pp. vi-ix), Lab Unit 1 (pp. 1-10), and Appendix A (pp. 197-201) before coming to the first Lab Meeting.&nbsp; <\/span><\/li><li><span style=\"font-size: 12pt;color: #000000\">Fill out and sign the Safety Statement (p. ix).<\/span><\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity One: Basic Microscopy<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Complete Activity One: Basic Microscopy (p. 2-3) <\/span><\/li><li><span style=\"font-size: 12pt\">View the following video:<a href=\"https:\/\/www.youtube.com\/watch?v=SUo2fHZaZCU\" target=\"_blank\" rel=\"noopener noreferrer\">&nbsp;Microscope Basic Set up and Use<\/a><\/span><\/li><li><span style=\"font-size: 12pt\">Use the <a href=\"http:\/\/www.ncbionetwork.org\/iet\/microscope\/\" target=\"_blank\" rel=\"noopener noreferrer\">Virtual Microscope<\/a><\/span>\n<ul>\n<li><span style=\"font-size: 12pt\">Click the &#8220;Learn&#8221; button to review what you learned in the Microscope video<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Click the &#8220;Explore&#8221; button to examine various slides. Start with &#8220;sample slides&#8221; and then view other slides.&nbsp;<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Click the &#8220;Test&#8221; button to assess your knowledge.<\/span><\/li>\n<\/ul>\n<\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity Two: Determining Total Magnification (p. 3)<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Follow the directions in the lab manual to complete Table 1-1 and include it in the pre-lab assignment.&nbsp;<\/span><\/li><\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity Three: Determining Field of View and Cell Size (p. 3-4)<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Review the directions for determining field of view in your lab manual.<\/span><\/li><li><span style=\"font-size: 12pt\">Preview the video: <a rel=\"noopener noreferrer\" href=\"https:\/\/www.youtube.com\/watch?time_continue=3&amp;v=_CkcYrns-6I&amp;feature=emb_title\" target=\"_blank\">Measuring with the Microscope<\/a><\/span> to understand what we will do together once we are in lab.<\/li><\/ul>\n\n\n\n<div style=\"height:41px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity Three: Determining Field of View and Cell Size (p. 3-4)<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Follow the <a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2022\/01\/Covid-19-Microscope-Cleaning.pdf\" target=\"_blank\">COVID-19 Safety Precautions for Compound Microscope Use<\/a> &#8211; see directions at each table.&nbsp;<\/span><\/li><li><span style=\"font-size: 12pt\">Review of compound microscope care and use.<\/span><\/li><li>Once in lab, <span style=\"font-size: 12pt\">students will use their own compound microscope and the letter &#8220;e&#8221; or newsprint prepared slide to Calculate Field of View and learn how to estimate Cell Size at different magnifications.<\/span><\/li><li><span style=\"font-size: 12pt\">A Review of this method can be seen in the following video: <a rel=\"noopener noreferrer\" href=\"https:\/\/www.youtube.com\/watch?time_continue=3&amp;v=_CkcYrns-6I&amp;feature=emb_title\" target=\"_blank\">Measuring with the Microscope<\/a><\/span><\/li><\/ul>\n\n\n\n<div style=\"height:36px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-white-color has-vivid-red-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2022\/08\/Field-of-View-Table1-2-updateBL.docx\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Updated Table 1-2 for Determining Field of Field of View in Lab (p.4)<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:38px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"has-text-align-center\"><strong>Updated Table 1-2. Field of view for each objective on the microscope<\/strong>.<\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td>Total Magnification<\/td><td>Ratio = 40\/Total Magnification<\/td><td>Calculated Field of View = <br>(Ratio)(Observed field of view at 40X) mm<\/td><\/tr><tr><td><\/td><td><\/td><td><\/td><\/tr><tr><td><\/td><td><\/td><td><\/td><\/tr><tr><td><\/td><td><\/td><td><\/td><\/tr><tr><td><\/td><td><\/td><td><\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000;font-family: UniversCondensed, Arial, sans-serif;font-size: 21px;font-weight: bold\">Activity Four: Examining Live Specimens (p. 5-11).&nbsp; &#8220;Organism X&#8221;.&nbsp;<\/span><\/h3>\n\n\n\n<div class=\"entry-content\">\n<figure><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-168 alignnone\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/diatom-300x225.jpg\" alt=\"diatom\" width=\"254\" height=\"190\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/diatom-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/diatom.jpg 500w\" sizes=\"auto, (max-width: 254px) 100vw, 254px\" \/>.\u00a0 <img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-169 alignnone\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/protist-2.jpg\" alt=\"protist\" width=\"265\" height=\"190\" \/><\/figure>\n<p>\u00a0\u00a0<\/p>\n<ol class=\"style2\">\n<li><span style=\"font-size: 12pt\">You will need the knowledge\/skills from Activities 1-3 (Pre-Lab) to effectively participate in Activity 4.\u00a0 Your TA will assign your table a particular Protist to investigate as Organism X.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Read the introduction to Activity 4.\u00a0 Each student will make their own slide of their assigned &#8220;Organism X&#8221;.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">In step #3 you should draw and record observations about the organism (p. 7). Observations are statements determined by using any one of a person\u2019s five senses (empirical reasoning). It is typically the first step in the Scientific Method and can lead to hypotheses.<\/span><\/li>\n<li class=\"style3\"><span style=\"font-size: 12pt\">Record your individual observations,\u00a0<\/span>\n<ul class=\"style3\">\n<li><span style=\"font-size: 12pt\">Label all notes and drawings with date, time, and place.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Record all results as you observe\/collect them; don\u2019t rely on memory.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Make sure to record what you SEE, not what you THINK should be there.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Detail is key, pay attention to even the smallest things.<\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"font-size: 12pt\">Once each group member has recorded their OWN observations, you will compare your observations with other students in your group. As a group, you will need to write a more comprehensive description of your organism (Table 1-3, page 9) that will be used by another group for making an identification of Organism X.\u00a0 These group observations cannot include any pictures or diagrams.\u00a0 \u00a0<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Another group will use your written description to identify the organism based solely on your written information (no sketches or pictures) and a set of microscope images of many possible protist species provided by your TA.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">If the other group cannot correctly identify your organism based on your group description, you will need to revise (edit) your descriptions until the correct organism is identified.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Groups should be prepared to defend the identification choice of the unknown organism.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Thoughtful and precise scientific communication is key to this activity and to all your laboratory work this semester.\u00a0 It is also vital to the peer review process \u2013 be it for class work or for publishing scientific literature.<\/span><\/li>\n<\/ol>\n<\/div>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-white-color has-vivid-red-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2022\/08\/Lab1_PostLab_Handout_BL.docx\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click to get Post-Lab Assignment<\/a><\/div>\n<\/div>\n\n\n\n\n\n<p><span style=\"font-size: 12pt\">Complete the Post_Lab assignment and turn this in on Moodle by the usual due date &#8211; 48 hours after the start of your regular lab day\/time.<\/span><\/p>\n\n\n\n<div style=\"height:41px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><\/div>\n\n\n\n\n\n<h3 class=\"style9 wp-block-heading\"><span style=\"color: #000000\">The Compound Light Microscope<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/cm501microscopediagram.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"469\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/cm501microscopediagram.jpg\" alt=\"microscope diagram\" class=\"wp-image-171\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/cm501microscopediagram.jpg 500w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/cm501microscopediagram-300x281.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/a><\/figure>\n\n\n\n<p><span style=\"font-size: 14pt\"><strong>Microscopy:<\/strong><\/span><\/p>\n\n\n\n<ul class=\"style3 wp-block-list\"><li class=\"style10\"><span style=\"font-size: 12pt\">Care and Use of Microscopes \u2013 See Appendix A in your Lab Manual<\/span><\/li><li class=\"style10\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=SUo2fHZaZCU\" target=\"_blank\" rel=\"noopener noreferrer\">Video on How to Use a Compound Microscope<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a href=\"http:\/\/micro.magnet.fsu.edu\/primer\/anatomy\/anatomy.html\" target=\"_blank\" rel=\"noopener noreferrer\">Basic Concepts of Microscopy<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?time_continue=3&amp;v=_CkcYrns-6I&amp;feature=emb_title\" target=\"_blank\" rel=\"noopener noreferrer\">Measuring with the Microscope&nbsp;<\/a><\/span> &#8211; how to <span style=\"font-size: 12pt\">determine the field of view and cell size<\/span><\/li><li class=\"style3\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/micro.magnet.fsu.edu\/primer\/java\/scienceopticsu\/powersof10\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">Exploring Magnification: Power of Ten video&nbsp;<\/a> &#8211; from 10 million light years away from the Milky Way down to 100 attometers!<\/span><\/li><li><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.ncbionetwork.org\/iet\/microscope\/\" target=\"_blank\" rel=\"noopener noreferrer\">Virtual Microscope<\/a><\/span><\/li><\/ul>\n","protected":false},"excerpt":{"rendered":"<p>General Lab Information &#8211; All lab units will be structured with Pre-Lab, In-Lab, and Post-Lab sections. You will work through some activities on your own before lab (Pre-Lab), other activities will be worked on as a group during your lab time (In-Lab), and there will generally be follow-up Post-Lab work.&nbsp; Be sure to check the [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":52,"menu_order":7,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2066","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2066","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/comments?post=2066"}],"version-history":[{"count":224,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2066\/revisions"}],"predecessor-version":[{"id":6638,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2066\/revisions\/6638"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/52"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/media?parent=2066"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}