{"id":2083,"date":"2020-05-20T01:09:06","date_gmt":"2020-05-20T01:09:06","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/?page_id=2083"},"modified":"2023-02-20T20:00:36","modified_gmt":"2023-02-20T20:00:36","slug":"lab-unit-3-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/laboratory-units\/lab-unit-3-online\/","title":{"rendered":"Lab Unit 3 &#8211; In Person"},"content":{"rendered":"\n<h2 class=\"has-pale-pink-color has-text-color wp-block-heading\"><span style=\"color: #000000\">Speciation: An Evolutionary Process &#8211; <span style=\"color: #0000ff\">In-Person Lab<\/span><\/span> <\/h2>\n\n\n\n<p><span style=\"font-size: 14pt\">Scroll below for In-Person Labs<\/span><\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/laboratory-units\/lab-3-online-make-up-module\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Module Lab 3<\/a><\/div>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-284\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/africanized-honeybee.jpg\" alt=\"Africanized honeybee\" width=\"470\" height=\"362\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/africanized-honeybee.jpg 470w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/africanized-honeybee-300x231.jpg 300w\" sizes=\"auto, (max-width: 470px) 100vw, 470px\" \/><\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Speciation &#8211; The evolutionary process in which new biological species arise.&nbsp;<\/span><\/h3>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\"><img loading=\"lazy\" decoding=\"async\" width=\"68\" height=\"77\" class=\"wp-image-2964 aligncenter\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/06\/bee_icon-300x273.jpg\" alt=\"bee icon\"><\/h2>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\">PRE-LAB<\/h2>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-white-color has-vivid-red-background-color has-text-color has-background\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/09\/Updated_Lab3_PreLab_handout.docx\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click to get the Pre-Lab Assignment<\/a><\/div>\n<\/div>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Read the lab unit, pp. 33-40. NOTE: Activity 1 &#8211; Procedure I (p. 36-37) will be done during the In-Lab meeting, but please read the whole unit ahead of lab.&nbsp;<\/span><\/li><li><span style=\"font-size: 12pt\">General Background Information <\/span>\n<ul>\n<li><span style=\"font-size: 12pt\">View the <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/09\/African_Bees_for_Bee_Keepers_UPDATED.pptx.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Hybridization of Honeybees Presentation<\/a><\/span><\/li>\n<\/ul>\n<\/li><li><span style=\"font-size: 12pt\">Activity 1 &#8211; Procedure II: Micropipetting and gel electrophoresis <\/span>\n<ul>\n<li><span style=\"font-size: 12pt\">View the following video and animation (make sure to review the lab manual, pp. 38-40):<br><\/span><span style=\"font-size: 12pt\"><a href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/gelelectrophoresis.html\" target=\"_blank\" rel=\"noopener noreferrer\">Gel Electrophoresis &#8211; animation<\/a><br><a href=\"https:\/\/www.youtube.com\/watch?v=2gfl8IbspWQ\" target=\"_blank\" rel=\"noopener noreferrer\">Instructions for loading an agarose gel<\/a> &#8211; video (mixing the same is not as important with our samples)<\/span><\/li>\n<\/ul>\n<\/li><li><span style=\"font-size: 12pt\">Speciation &#8211; View the following:<\/span>\n<ul>\n<li><span style=\"font-size: 12pt\"><a style=\"background-color: #ffffff\" href=\"https:\/\/learn.genetics.utah.edu\/content\/evolution\/species\" target=\"_blank\" rel=\"noopener noreferrer\">What is a Species?<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a style=\"background-color: #ffffff\" href=\"https:\/\/learn.genetics.utah.edu\/content\/evolution\/bears\/\" target=\"_blank\" rel=\"noopener noreferrer\">Bears, Species, and DNA<\/a> <\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.bbc.com\/news\/science-environment-37311716\" target=\"_blank\" rel=\"noopener noreferrer\">Giraffe Genetic Secrets: Four Species of Tallest Mammal Identified<\/a><\/span><\/li>\n<\/ul>\n<\/li><li><span style=\"font-size: 12pt\"><strong>NOTE:<\/strong> Be sure to bring your lab manual to lab this week.&nbsp; This lab unit is not available digitally.<\/span><\/li><\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">The Roles of different bees<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1008\" height=\"540\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/beecastes.jpg\" alt=\"Bee Caste Chart\" class=\"wp-image-369\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/beecastes.jpg 1008w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/beecastes-300x161.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/beecastes-768x411.jpg 768w\" sizes=\"auto, (max-width: 1008px) 100vw, 1008px\" \/><\/figure>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\"><img loading=\"lazy\" decoding=\"async\" width=\"68\" height=\"77\" class=\"wp-image-2964 aligncenter\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/06\/bee_icon-300x273.jpg\" alt=\"bee icon\"><\/h2>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\">IN-LAB<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Morphological Characteristics &#8211; Activity 1 &#8211; Procedure-I: (p. 36-37)<\/span><\/h3>\n\n\n\n<ol class=\"wp-block-list\"><li><span style=\"font-size: 12pt\"><strong>Working in the lab this week, <\/strong>each group will get a notebook containing 36 Bee wing pictures. The pictures in each notebook at the 6 different lab tables are different from one another.&nbsp; Two tables of students will assess the bees from one hive in order to determine if the bees in that hive have been hybridized and\/or taken over by Africa bees.&nbsp; Why would this be problematic for a beekeeper?<\/span> <p style=\"text-align: center\"><span style=\"color: #000000;font-size: 12pt\"><strong>Bee Wing Picture Assignments:<\/strong><\/span><\/p> <table style=\"width: 100%;border-collapse: collapse;border-style: solid\"> <tbody> <tr> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 1 &#8211; Group 1<\/span><\/td> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 1 &#8211; Group 2<\/span><\/td> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 2 &#8211; Group 3<\/span><\/td> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 2 &#8211; Group 4<\/span><\/td> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 3 &#8211; Group 5<\/span><\/td> <td style=\"width: 16.6667%;border-style: solid;text-align: center\"><span style=\"font-size: 12pt\">Hive 3 &#8211; Group 6<\/span><\/td> <\/tr> <\/tbody> <\/table> <p>&nbsp;<\/p> <\/li><li><span style=\"font-size: 12pt\">Use the provided metric ruler (cm\/mm) on your lab table.<\/span><\/li><li><span style=\"font-size: 12pt\">Study the provided wing diagram in the notebook or lab manual to see how to position the ruler <strong>parallel<\/strong> to the wing length to determine the length from the notch near the joint to the longest point on the flattened wing.&nbsp; <strong>Do not<\/strong> <strong>angle<\/strong> the ruler to connect the notch to the longest part of the wing. You may need to rotate some of the images for ease of measuring.<\/span><\/li><li><span style=\"font-size: 12pt\"><a rel=\"noopener noreferrer\" href=\"https:\/\/ncsu.hosted.panopto.com\/Panopto\/Pages\/Viewer.aspx?id=557498cf-6658-4b58-b785-acb600a7b48c\" target=\"_blank\">Video<\/a> on how to measure the bee wing images.<\/span><\/li><li><span style=\"font-size: 12pt\">As a group, divide the wings to measure evenly between each student in your group.&nbsp; If you have 4 group members, each person should measure 9 different wings.&nbsp; There are 12 Known African wings, 12 Known European wings, and 12 Unknown Wings. You will be comparing the length of unknown wings to the other two groups of known bees.<\/span><\/li><li><span style=\"font-size: 12pt\">Record your group data and calculate the average wing lengths on page 37, Table 3-2 in your lab manual.<\/span><\/li><li><span style=\"font-size: 12pt\">Graph the data. Use the provided Bee Wing Length Graphing Excel or Google Sheet in your Group folder or on one of the lab computers.     &nbsp; <a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2022\/09\/Downloadable_Lab3_wingsgraphRESETtemplateF22.xls\" target=\"_blank\">Excel Template.<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a rel=\"noopener noreferrer\" href=\"https:\/\/ncsu.hosted.panopto.com\/Panopto\/Pages\/Viewer.aspx?id=28512997-14ca-4ab5-8c86-ae7a00eff722\" target=\"_blank\">Video<\/a> on entering data in the Graphing Google Sheet.&nbsp; The video refers to using an Excel template, but we found the transfer of the Excel sheet to work on in Google sheets does not keep all the settings in our template.&nbsp; Entering the data in the the provided Google Sheet template is the same.&nbsp;<\/span><\/li><\/ol>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Molecular Characteristics: (p. 38-41)<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\">In lab we will run DNA Electrophoresis using samples of mtDNA from bees found in your assigned hive.<\/span><\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000;font-size: 12pt\"><strong>Equipment:<\/strong><\/span><\/h4>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/DNA-Unit-e1590465732367.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"275\" height=\"261\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/DNA-Unit-e1590465732367.jpg\" alt=\"\" class=\"wp-image-2149\" \/><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/Micropipette_in_action.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"235\" height=\"300\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/Micropipette_in_action-235x300.jpg\" alt=\"\" class=\"wp-image-2150\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/Micropipette_in_action-235x300.jpg 235w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/05\/Micropipette_in_action.jpg 440w\" sizes=\"auto, (max-width: 235px) 100vw, 235px\" \/><\/a><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000;font-size: 12pt\"><strong>The Electrophoretic Process<\/strong> &#8211; You should have viewed the electrophoresis simulation as part of your pre-lab.<\/span><\/h4>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000;font-family: UniversCondensed, Arial, sans-serif;font-size: 21px;font-weight: bold\">Compare your morphological data with the corresponding mtDNA analysis.&nbsp;<\/span><\/h4>\n\n\n\n<p><span style=\"font-size: 12pt\">As a group of 4, be prepared to present your findings to the class.&nbsp; What can you conclude about the bees in your hive?&nbsp; Explain your supporting evidence.&nbsp; How do your results compare with the other Group investigating this hive?&nbsp; What course of action do you suggest to the Beekeeper of this hive?<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\">What economic implications are at stake making appropriate hive management so important?<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt;color: #ff0000\"><strong><em>(Before leaving Lab &#8211; your TA will review the methods for random sampling and measuring leaf surface area needed for the Lab Unit 4.1 fieldwork due in the next Pre-Lab.&nbsp;<\/em><\/strong><\/span><\/p>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\"><img loading=\"lazy\" decoding=\"async\" width=\"68\" height=\"77\" class=\"wp-image-2964 aligncenter\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/06\/bee_icon-300x273.jpg\" alt=\"bee icon\"><\/h2>\n\n\n\n<h2 class=\"has-text-align-center wp-block-heading\">POST-LAB<\/h2>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\">Use your cell phone to take a picture of your stained gel (saved as a jpeg or pdf) and include it with your Group Graph and explanation of what this means and what your advise would be to the bee keeper in a Word or Google doc.&nbsp; Submit this in Moodle (group work &#8211; one submission per group-people at your table).&nbsp;<\/span><\/li><li><span style=\"font-size: 12pt\">Answer the questions #1-9 on pages 43-46 (group work &#8211; one submission per group &#8211; people at your table).&nbsp;<\/span><\/li><li><span style=\"font-size: 12pt\">Need to start work on the Lab 4.1 Leaf Herbivory Data Collection\/PRE-LAB assignment.&nbsp; See the assignment box below. (<span style=\"color: #000000\">Individual work).&nbsp;<\/span><\/span><\/li><\/ul>\n\n\n\n\n\n<div style=\"height:31px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><\/p>\n\n\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Bee Background Information:<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\"><li><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.ncagr.gov\/plantindustry\/plant\/apiary\/ncbee.htm\" target=\"_blank\" rel=\"noopener noreferrer\">Responding to the Introduction of Africanized Honey Bees (AHB)<\/a><br><\/span><\/li><li><span style=\"font-size: 12pt\"><a href=\"https:\/\/entomology.ces.ncsu.edu\/2020\/04\/host-shifts-and-honey-bees-lessons-from-covid-19\/\" target=\"_blank\" rel=\"noopener noreferrer\">Host-Shifts &amp; Honey Bees: Lessons from COVID-19<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a href=\"https:\/\/content.ces.ncsu.edu\/africanized-honey-bees-some-questions-and-answers\" target=\"_blank\" rel=\"noopener noreferrer\">Africanized Honey Bees Some Questions and Answers (NC State Extension)<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a style=\"background-color: #ffffff\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/Schneider_et_al_20062.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">The African Honey Bee \u2013 3 papers<\/a><\/span><\/li><li><span style=\"font-size: 12pt\"><a style=\"background-color: #ffffff\" href=\"http:\/\/entnemdept.ufl.edu\/creatures\/misc\/bees\/ahb.htm\" target=\"_blank\" rel=\"noopener noreferrer\">Africanized Bees in Florida<\/a><\/span><\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"408\" height=\"500\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/02\/beeIllustrate1.gif\" alt=\"Identification of Bees and Wasps\" class=\"wp-image-190\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Speciation Information:<\/span><\/h3>\n\n\n\n<p><a style=\"font-size: 12pt;background-color: #ffffff\" href=\"https:\/\/learn.genetics.utah.edu\/content\/evolution\/species\" target=\"_blank\" rel=\"noopener noreferrer\">What is a Species?<\/a><span style=\"font-size: 12pt\">&nbsp;<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/learn.genetics.utah.edu\/content\/evolution\/bears\/\" target=\"_blank\" rel=\"noopener noreferrer\">Bears, Species and DNA<\/a>&nbsp;<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.bbc.com\/news\/science-environment-37311716\" target=\"_blank\" rel=\"noopener noreferrer\">Giraffe Genetic Secrets: Four Species of Tallest Mammal Identified<\/a><\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Electrophoresis Information<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.labxchange.org\/library\/pathway\/lx-pathway:33b08759-5d13-4128-8867-68428a8d1081\/items\/lx-pb:33b08759-5d13-4128-8867-68428a8d1081:lx_simulation:644d8922\" target=\"_blank\" rel=\"noopener noreferrer\">Gel Electrophoresis Simulation<\/a><br><\/span><span style=\"font-size: 12pt\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/gelelectrophoresis.html\" target=\"_blank\" rel=\"noopener noreferrer\">How DNA Electrophoresis works<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Speciation: An Evolutionary Process &#8211; In-Person Lab Scroll below for In-Person Labs Speciation &#8211; The evolutionary process in which new biological species arise.&nbsp; PRE-LAB Read the lab unit, pp. 33-40. NOTE: Activity 1 &#8211; Procedure I (p. 36-37) will be done during the In-Lab meeting, but please read the whole unit ahead of lab.&nbsp; General [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":52,"menu_order":42,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2083","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2083","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/comments?post=2083"}],"version-history":[{"count":182,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2083\/revisions"}],"predecessor-version":[{"id":6573,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/2083\/revisions\/6573"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/52"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/media?parent=2083"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}