{"id":80,"date":"2018-02-14T03:09:27","date_gmt":"2018-02-14T03:09:27","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/?page_id=80"},"modified":"2020-06-14T15:32:08","modified_gmt":"2020-06-14T15:32:08","slug":"laboratory-9","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/laboratory-units\/laboratory-9\/","title":{"rendered":"Lab Unit 10 &#8211; Online"},"content":{"rendered":"<h2><span style=\"color: #000000\">Diversity in Transport\u00a0 (a 2 Week lab unit)<\/span> <span style=\"color: #000000\">&#8211; Online &#8211;<\/span><span style=\"color: #ff00ff\">working copy??<\/span><\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-294\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/03\/images.jpg\" alt=\"stomata\" width=\"267\" height=\"212\" \/><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-802 alignleft\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/xylem.gif\" alt=\"moving water molecules\" width=\"320\" height=\"176\" \/><\/p>\n<h3 align=\"left\"><\/h3>\n<h3 align=\"left\"><\/h3>\n<h3 align=\"left\"><\/h3>\n<h3 align=\"left\"><\/h3>\n<h3><\/h3>\n<h3><span style=\"color: #ff00ff\">?Change format, Pre-Lab, In-Lab, Post Lab<\/span><\/h3>\n<p>[enhanced-heading heading=&#8221;PRE-LAB&#8221; headingtype=&#8221;h5&#8243; headingstyle=&#8221;h2&#8243; target=&#8221;_top&#8221; icon=&#8221;ucomm-heart&#8221; iconstyle=&#8221;circle-filled&#8221; iconposition=&#8221;stacked&#8221; coloroptions=&#8221;text&#8221; textcolor=&#8221;wolfpackred&#8221; fontface=&#8221;universlight&#8221; \/]<\/p>\n<h3><span style=\"color: #0000ff\"><span style=\"color: #ff00ff\">[insert pre-lab assignment handout]<\/span><\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">Activity 1: Transpiration in Higher Plants (p. 168-169).<\/span>\n<ul>\n<li><span style=\"font-size: 12pt\">Focus on Plant Anatomy and Transpiration\/Translocation &#8211; substitute the potometer procedure with Simulation from Glencoe (requires FlashPlayer) <span style=\"color: #ff00ff\">OR try Pratt simulation to examine many factors &#8211; PS, Cell Respiration, Absorption, Transpirations and Translocations. -make an assignment for this?\u00a0 Not sure what Erica did with this.??\u00a0 No longer graphing data, but looking more holistically???<\/span><\/span><\/li>\n<\/ul>\n<\/li>\n<li><span style=\"font-size: 12pt\">\u00a0Activity 2: Earthworm Dissection (p. 175-176)<\/span><\/li>\n<li><span style=\"font-size: 12pt\">\u00a0Activity 3: Crayfish Dissection (p. 176-179.<\/span><\/li>\n<\/ul>\n<h4><span style=\"color: #ff0000\">I will reorganize\/move the content below once I know what is Pre-Lab, In-Lab, Post-Lab AND if 1 or 2 weeks of Transport.<\/span><\/h4>\n<h3><span style=\"color: #ff00ff\">Work on Week 2 IN-LAB ???? if we do this all in one week?<\/span><\/h3>\n<ul>\n<li>Focus on Heart?<\/li>\n<\/ul>\n<p>[enhanced-heading heading=&#8221;POST-LAB&#8221; headingtype=&#8221;h5&#8243; headingstyle=&#8221;h2&#8243; target=&#8221;_top&#8221; icon=&#8221;ucomm-heart&#8221; iconstyle=&#8221;circle-filled&#8221; iconposition=&#8221;stacked&#8221; coloroptions=&#8221;text&#8221; textcolor=&#8221;wolfpackred&#8221; fontface=&#8221;universlight&#8221; \/]<\/p>\n<h4><span style=\"color: #ff00ff\">I will reorganize the content below once I know what is Pre-Lab, In-Lab, Post-Lab AND if 1 or 2 weeks of Transport.<\/span><\/h4>\n<h4><span style=\"color: #000000\">Lab Information- Please read the Lab Unit 10 week 1 (pages 167-182), work through all activities and answer questions in your lab manual using the lab text and website.\u00a0<\/span><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Transport_Unit-10wkI_online_sp2020F.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color: #ff0000\">Read and follow this outline modification for the Transport lab week.<\/span><\/a><\/h4>\n<h2 class=\"style5\" align=\"left\">Transport in Plants: (Week 1)<\/h2>\n<h3><span style=\"color: #000000\">Background:<\/span><\/h3>\n<ul class=\"style6\">\n<li class=\"style6\"><span style=\"font-size: 12pt\">What is transported?<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">Which tissues (xylem and phloem) are used for Transpiration? for Translocation?<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">What is the difference between Transpiration and Translocation?<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">What drives these types of transport?<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">Leaf Anatomy\u00a0<span class=\"style6\">(though stomates are not generally found on the upper epidermis as seen here, this diagram nicely shows important cell layers and gas exchange)<\/span><\/span><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-509 size-full\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/leafcrosssection.gif\" alt=\"leaf cross section\" width=\"649\" height=\"411\" \/><\/p>\n<ul class=\"style6\">\n<li><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=cFX4JrsPaUs\">Stomata opening and closing<\/a>\u00a0&#8211; videoclip (1:07)<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.bbc.co.uk\/programmes\/p00lxt8k\">Water Transport in Plants<\/a>\u00a0&#8211; short video David Attenborough (3:05) -video continues to 4:44, but not as applicable to our experiment.<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=CmBDVIUB19g\" target=\"_blank\" rel=\"noopener noreferrer\">Plant Transport: Xylem, Phloem and Transpiration (3D Animation)<\/a> (5:36) More detailed than needed for lab, but very good and may be helpful for understanding the background if not yet covered in lecture.<\/span><\/li>\n<\/ul>\n<p><strong><span style=\"font-size: 18pt\">We will look more closely at structures involved in both Transpiration and Translocation in plant leaves and stem cross sections.<\/span><\/strong><\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/VascTissDrawing.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1706 aligncenter\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/VascTissDrawing.jpg\" alt=\"\" width=\"416\" height=\"453\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/VascTissDrawing.jpg 941w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/VascTissDrawing-276x300.jpg 276w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/VascTissDrawing-768x836.jpg 768w\" sizes=\"auto, (max-width: 416px) 100vw, 416px\" \/><\/a><\/p>\n<p><span style=\"font-size: 14pt\"><strong><em>Text and Figures in the Lab Manual pages 95-96 and 169 will be very helpful for interpreting and labeling your drawings of the following slides.<\/em><\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt\"><strong>Use the empty space on page 180 to draw and label the pictures #1, #2, #3, and #4 below \u2013<\/strong> Sp2020.<\/span><\/p>\n<p>#1.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/ZebrinaStomata.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1975\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/ZebrinaStomata-263x300.jpg\" alt=\"\" width=\"263\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/ZebrinaStomata-263x300.jpg 263w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/ZebrinaStomata-768x877.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/ZebrinaStomata-896x1024.jpg 896w\" sizes=\"auto, (max-width: 263px) 100vw, 263px\" \/><\/a>.\u00a0 #2.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Cross-section-MC.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1978\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Cross-section-MC-300x218.jpg\" alt=\"\" width=\"402\" height=\"292\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Cross-section-MC-300x218.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Cross-section-MC.jpg 640w\" sizes=\"auto, (max-width: 402px) 100vw, 402px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt\"><strong>Draw and label the following prepared slides of plant tissues. &#8211; Sp2020<\/strong><\/span><\/p>\n<ol>\n<li><span style=\"font-size: 12pt\"><strong>Picture #1 <\/strong>is a view of the lower epidermis of <strong><em>Zebrina<\/em><\/strong> .\u00a0 It is from a whole mount or epidermal peel preparation.<\/span><\/li>\n<\/ol>\n<ul>\n<li><span style=\"font-size: 12pt\">Make a fresh epidermal leaf peels of a <em>Zebrina<\/em>\u00a0leaf to study stomate structure. <\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-size: 12pt\">Peel the leaf to expose the lower epidermis without other cell layers.\u00a0 If you cannot get a good leaf peel, then make a wet mount of a thin area of the leaf.\u00a0 <\/span><\/li>\n<li><span style=\"font-size: 12pt\">Place the peel or leaf piece epidermis side up (more purple side) facing UP on the slide.\u00a0 Then make a wet mount (top with a drop of water and a coverslip).\u00a0 View with the compound microscope \u2013 medium to high power.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Students should sketch what they see on the lower epidermis. Structures to draw and label:\u00a0epidermal cells, guard cells, stoma (= <em>singular<\/em>) or stomata\/stomates (= <em>plural<\/em>).<\/span><\/li>\n<\/ul>\n<ol start=\"2\">\n<li><span style=\"font-size: 12pt\"><strong>Leaf Cross section slide (#2) <\/strong>\u2013 Fig. 6-2, p. 95 and Fig. 10-2 p. 169 &#8211; Structures to draw and label: cuticle, upper and lower epidermis, guard cells (stoma), mesophyll (contains spongy mesophyll and palisade layers), vein or midvein containing xylem and phloem.\u00a0 \u00a0\u00a0<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><strong>Herbaceous (#3) <\/strong>and <strong>Woody Stem slide (#4)<\/strong> \u2013 Fig. 6-4, p. 96 and Fig. 10-1, p. 169 \u2013 Epidermis, cortex, pith, vascular bundles in herbaceous stems (xylem\/inside and phloem\/towards outside of bundles), in woody stem xylem collects as inner stem annual rings (eventually growth rings\/wood), phloem to the outside under the cork (in woody stems), epidermis layer.<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><strong><em>Tilia<\/em> (# 4) <\/strong>woody stem sections showing 1, 2, 3 &amp; 4 growth rings \u2013 Same woody stem as the slide in #3 above) \u2013 Fig. 6-4A, p. 96 &#8211; should be able to see most of the same structures as the woody stem above, but more developed in the older stem sections \u2013pith is smaller on the inside, more developed annual rings\/xylem accumulation with age that becomes wood, phloem to the outside of the xylem rings being crushed under the cork\/epidermis layers, cortex less and less apparent under outmost layers.<\/span><\/li>\n<\/ol>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem.jpg\"><span style=\"color: #000000\">#3<\/span> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1708\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem-300x274.jpg\" alt=\"\" width=\"300\" height=\"274\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem-300x274.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem-768x703.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem-1024x937.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/HerbStem.jpg 1584w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\u00a0 \u00a0 <span style=\"color: #000000\">#4<\/span>\u00a0 <\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/Tilia3YrStem.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1710\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2019\/11\/Tilia3YrStem.jpg\" alt=\"\" width=\"394\" height=\"222\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><strong><span style=\"font-size: 18pt\">Spring 2020: Due to the change to online labs, we will not be able to do Activity One in the lab manual.\u00a0 Instead we will use the following simulation.<\/span><\/strong><\/p>\n<p><span style=\"font-size: 18pt\"><a href=\"http:\/\/glencoe.mheducation.com\/sites\/dl\/free\/0078802849\/383946\/BL_10.html\" target=\"_blank\" rel=\"noopener noreferrer\">Simulation of Plant Transpiration Experiment<\/a> <span style=\"font-size: 12pt\">&#8211; you may need to enable Flash Player.<\/span><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">Follow the directions as you read and scroll down using the information on the left side in the simulation.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Work through the simulation selecting all 4 plants and working through the control and 3 different treatments for each.\u00a0 Collect the data after each &#8220;run&#8221;.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Use the data to make ONE graph including data from all 4 plants under control and all treatment\/environmental conditions.\u00a0 Be sure to include all of the appropriate components of a proper graph (see <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/08\/Aooendix-D-Excel-Mac2018.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Appendix D Exce<\/a><span style=\"color: #993300\">l<span style=\"color: #000000\"> in the lab manual<\/span><\/span>).<\/span><\/li>\n<\/ul>\n<h3 class=\"style5\" style=\"text-align: left\"><span style=\"color: #000000;font-family: UniversCondensed, Arial, sans-serif;font-size: 21px;font-weight: bold\">Cool Articles\/links About Researchers Using Plants for Phytoremediation<\/span><\/h3>\n<ul>\n<li class=\"style6\"><span style=\"font-size: 12pt\">Researchers in Elizabeth City NC &#8211;\u00a0<a href=\"https:\/\/news.ncsu.edu\/2009\/09\/green-clean\/\">&#8216;Green Clean:&#8217; Researchers Determining Natural Ways to Clean Contaminated Soil<\/a><\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/nowak_trees.pdf\">The Effects of Urban Trees on Air Quality<\/a> (short article)<\/span><\/li>\n<li><span class=\"style6\" style=\"font-size: 12pt\"><a href=\"http:\/\/ea.cals.ncsu.edu\/online-graduate-programs\/master-of-environmental-assessment\/\">NCSU Master of Environmental Assessment Degree Program<\/a><\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2>Transport in Animals: (Week 1)<\/h2>\n<h2>Open and Closed Circulatory Systems<\/h2>\n<p><span style=\"font-size: 18pt\"><em>Activity 3 (p. 177-179) Crayfish &#8211; open circulatory system<\/em><\/span><\/p>\n<ul class=\"style6\">\n<li><span style=\"font-size: 12pt\"><span class=\"style2\"><strong>Open circulatory system &#8211;\u00a0<\/strong><\/span>The open circulatory system is common to invertebrates. In an open circulatory system, the blood is pumped into a hemocoel and diffuses back to the circulatory system between cells. Blood is pumped into body cavities by a heart.\u00a0 <a href=\"https:\/\/www.youtube.com\/watch?v=Cq--zXVc8Ww&amp;feature=related\">See hemolymph circulation in Insects (0:48)<\/a>, discussion of <a href=\"http:\/\/www.youtube.com\/watch?v=Lv781axxV5o http:\/\/www.youtube.com\/watch?v=Lv781axxV5o\">circulation in a cockroach<\/a> (1:45).<\/span><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-513\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/opensystem.gif\" alt=\"open circulatory system\" width=\"227\" height=\"164\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt\"><em><strong>Crayfish Dissection Images<\/strong><\/em><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/ExtAnatAnterior.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">External Anatomy<\/a>: Anterior<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/ExterAnatPost.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">External Anatomy<\/a>: Posterior<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/mouthparts.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">External Anatomy<\/a>: Mouthparts<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/InterAnatLateral.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Internal Anatomy<\/a>: Lateral<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/InterAnatDoralAnt.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Internal Anatomy<\/a>: Dorsal Anterior<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/FemaleCrayfish.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Female Crayfish<\/a>: Ventral<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/MaleCrayfish-1.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Male Crayfish<\/a>: Ventral<\/span><\/li>\n<\/ul>\n<p><strong><em><span style=\"font-size: 12pt\">Videos of Preserved Crayfish Dissections\u00a0<\/span><\/em><\/strong><\/p>\n<ul>\n<li class=\"style6\"><span style=\"font-size: 12pt\">Crayfish Dissections &#8211; <a href=\"http:\/\/www.youtube.com\/watch?v=2cBGuEDxvNo\" target=\"_blank\" rel=\"noopener noreferrer\">External<\/a>\u00a0Anatomy (8:52) and\u00a0<a href=\"http:\/\/www.youtube.com\/watch?v=wSGEt1mY2OM\" target=\"_blank\" rel=\"noopener noreferrer\">Internal<\/a>\u00a0Anatomy (5:55)<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=-XOKPCGh17Y\" target=\"_blank\" rel=\"noopener noreferrer\">Crayfish Dissection<\/a>\u00a0&#8211; Includes both the external and internal anatomy (7:14)<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 18pt\"><em>Activity 2 (p. 175-176) Earthworm &#8211; closed circulatory system<\/em><\/span><\/p>\n<ul class=\"style6\">\n<li><span class=\"style6\"><span style=\"font-size: 12pt\"><strong>Closed circulatory system &#8211;\u00a0<\/strong>Vertebrates, and some invertebrates, have a closed circulatory system. In a closed circulatory system, the blood is in closed in a vessel at all times. The blood is pumped by a heart and travels through each of the closed vessels. The blood is not typically pumped into any body cavities.\u00a0<\/span><br \/>\n<\/span><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-514\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/closedsystem.gif\" alt=\"closed circulatory system\" width=\"227\" height=\"161\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 18pt\"><em><strong>Earthworm\u00a0<\/strong><\/em><\/span><\/p>\n<p><span style=\"font-size: 12pt\">Note the closed circulatory system<\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-821 alignleft\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/earthwm1-300x93.gif\" alt=\"\" width=\"400\" height=\"124\" \/><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-515 size-full alignleft\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/earthworm.jpg\" alt=\"earthworm\" width=\"629\" height=\"377\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/earthworm.jpg 629w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/earthworm-300x180.jpg 300w\" sizes=\"auto, (max-width: 629px) 100vw, 629px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><strong><em><span style=\"font-size: 18pt\"><a href=\"http:\/\/glencoe.mheducation.com\/sites\/dl\/free\/0078802849\/383950\/BL_14.html\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Earthworm Dissection Animation<\/span><\/a><span style=\"font-size: 12pt\"> &#8211; You may need to enable Flash Player.<\/span><\/span><\/em><\/strong><\/p>\n<p><strong><em><span style=\"font-size: 18pt\"><span style=\"font-size: 12pt\">You will need to take screen shots of your fully labeled External and Internal Earthworm Anatomy to submit with your assignment.<\/span><\/span><\/em><\/strong><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">Label the indicated structures for external anatomy in this animation.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Label the indicated structures for internal anatomy in this animation.<\/span><\/li>\n<\/ul>\n<p><strong><em><span style=\"font-size: 12pt\">Videos of Preserved Earthworm dissections<\/span><\/em><\/strong><\/p>\n<ul>\n<li><a style=\"font-size: 12pt;background-color: #ffffff\" href=\"https:\/\/www.youtube.com\/watch?v=aCnwF6vtE2g\" target=\"_blank\" rel=\"noopener noreferrer\">Preserved Earthworm Dissection Video<\/a><span style=\"font-size: 12pt\"> &#8211; external and internal anatomy (7:27)<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=u9HHS1uPFSo&amp;NR=1&amp;feature=endscreen\" target=\"_blank\" rel=\"noopener noreferrer\">Earthworm Dissection video<\/a>\u00a0(8:49<\/span>)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>[callout heading=&#8221;Transport Week 1 Assignment&#8221; headingicon=&#8221;noicon&#8221; textalign=&#8221;textcenter&#8221; url=&#8221;https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/BIO-181-Transport-1-Assignment-Sp2020.docx&#8221; target=&#8221;true&#8221; type=&#8221;basic&#8221; bgcolor=&#8221;huntyellow&#8221;]Use the attached Word guidelines\/template to create ONE document to turn into your TA via Moodle as a PDF.  This is due April 13-17 by midnight\/11:59PM on the day of your regular lab section.  You will work your way through all the activities and questions in the Transport Week 1 lab using the lab manual and the lab website.  This assignment is worth 10 points.[\/callout]<\/p>\n<h3><span style=\"color: #ff00ff\">???I could make this a separate page OR add in Pre, In and Post lab icons here.\u00a0 I think these were kept as ONE page since the heart info spans 2 lab units.<\/span><\/h3>\n<h2 class=\"style5\">Transport in Animals: (Week 2)<\/h2>\n<h4><span style=\"color: #000000\">Lab Information- Please read the Lab Unit 10 week 2 (pages 183-195), work through all activities and answer questions in your lab manual using the lab text and website. <\/span><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Transport__wkII_online_sp2020.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color: #ff0000\">Read and follow this outline modification for the Transport lab week.<\/span><\/a><\/h4>\n<h4><span style=\"color: #000000\">You will label drawings of plant structures, perform a Transpiration simulation, graph your data and answer questions that you will submit to your TA in Moodle.<\/span><\/h4>\n<h4><span style=\"color: #339966\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/03\/181_Lab10_F19-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">???*<\/a>If you do not have your lab manual, you can print out the indicated pages (questions and diagrams) or write all your work out on paper to save and use for studying for your Unit 4 Quiz\/Assignment that will be given in the lecture Moodle class April 23 (sect. 001) and 24 (sect. 002) lecture sections.<\/span><\/h4>\n<h2 class=\"style5\">Mammalian Circulation<\/h2>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-516\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/heart.jpg\" alt=\"heart\" width=\"267\" height=\"245\" \/><\/p>\n<p class=\"style5\"><span style=\"font-size: 12pt\">We will focus this week of lab on:<\/span><\/p>\n<ul>\n<li class=\"style6\"><span style=\"font-size: 12pt\">the 4 chambers of the heart<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">right vs. left<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">where did blood come from when entering the heart? -&gt; right atria, -&gt; left atria?<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">where does it go when it leaves the heart? -&gt; right ventricle, -&gt; left ventricle?<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">where is the blood oxygenated and deoxygenated<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">pulmonary vs. systemic circulation<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">interpreting EKGs (what occurs at the PQRST peaks).\u00a0<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\">how does activity affect heart rate (BPM) and why?\u00a0\u00a0<\/span><\/li>\n<\/ul>\n<h3><span style=\"color: #000000\">Activity One: Heart Structure and Double Circulation (Pulmonary and Systemic)<\/span><\/h3>\n<h3><span style=\"color: #000000\">Animation &#8211;<\/span>\u00a0<a href=\"http:\/\/anatomycorner.com\/main\/2016\/12\/14\/heart-systole-and-diastole\/\" target=\"_blank\" rel=\"noopener noreferrer\">Heart &#8211; Systole and Diastole\u00a0<\/a><\/h3>\n<h3><span style=\"color: #000000\">Video &#8211;<\/span>\u00a0<a href=\"https:\/\/www.youtube.com\/watch?v=uJC358hDVWQ&amp;list=PLWpMSgyE6WBWsBvdlWYT6RYOcGFpE35I4\" target=\"_blank\" rel=\"noopener noreferrer\">External Anatomy of the Heart and Blood Flow (using a heart model)<\/a><\/h3>\n<h3><span style=\"color: #000000\">Video &#8211;<\/span>\u00a0<a href=\"https:\/\/www.youtube.com\/watch?v=PfvR4N_pYYg&amp;list=PLWpMSgyE6WBWsBvdlWYT6RYOcGFpE35I4&amp;index=3\" target=\"_blank\" rel=\"noopener noreferrer\">Internal Anatomy of the Heart and Blood Flow (using a heart model)<\/a><\/h3>\n<p>&nbsp;<\/p>\n<h3><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-817\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/circulation1322509656736-286x300.jpg\" alt=\"\" width=\"481\" height=\"504\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/circulation1322509656736-286x300.jpg 286w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/circulation1322509656736-768x805.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/circulation1322509656736.jpg 964w\" sizes=\"auto, (max-width: 481px) 100vw, 481px\" \/><\/h3>\n<h3><span style=\"color: #000000\"><strong>Mammalian Heart &#8211; Anatomy and Function &#8211; helpful links<\/strong><\/span><\/h3>\n<ul>\n<li class=\"style6\"><a href=\"https:\/\/www.sciencelearn.org.nz\/resources\/2355-labelling-the-heart\">L<span style=\"font-size: 12pt\">abel a heart<\/span><\/a><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.sumanasinc.com\/webcontent\/anisamples\/biology\/human_heart.html\" target=\"_blank\" rel=\"noopener noreferrer\">View pulmonary circulation<\/a><\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/inference-for-mu.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Human Circulatory System<\/a> (images from Human Anatomy, 2nd Ed. McKinley and O&#8217;Loughlin)<\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=LqhvmUEdOYY\" target=\"_blank\" rel=\"noopener noreferrer\">Circulation Rap\/Song<\/a><\/span><\/li>\n<li class=\"style6\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.zerobio.com\/videos\/sheep_heart_anatomy.html\" target=\"_blank\" rel=\"noopener noreferrer\">Sheep heart dissection <\/a>&#8211; similar to what you would have seen in lab<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=LySZtgYK4gw\" target=\"_blank\" rel=\"noopener noreferrer\">Living with an Artificial Heart: Prince&#8217;s Journey<\/a> (5:45)<\/span><\/li>\n<li><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=hBjeS6CXnsM\" target=\"_blank\" rel=\"noopener noreferrer\">Could artificial hearts make donations obsolete?<\/a> (4:16)<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000\">Activity Two: Heart Function and ECGs<\/span><\/h3>\n<h3><a href=\"https:\/\/medmovie.com\/library_id\/7556\/topic\/cvml_0038a\/\" target=\"_blank\" rel=\"noopener noreferrer\">Electrocardiogram<\/a> &#8211; nice background information on electrical activity in the heart (1:20)<\/h3>\n<h3><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-518\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/04\/ecg03-1.gif\" alt=\"ECG\" width=\"477\" height=\"311\" \/>\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"wp-image-819 alignnone\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2018\/05\/ECG1_Graph.gif\" alt=\"\" width=\"253\" height=\"190\" \/><\/p>\n<p><strong>ECG peaks and heartbeats<\/strong><\/p>\n<p>&nbsp;<\/p>\n<h3><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-content\/uploads\/sites\/75\/2020\/04\/Student-ECG.png\" target=\"_blank\" rel=\"noopener noreferrer\">ECG <span style=\"color: #000000\">&#8211; Example of a student ECG from our lab (recording over 10 seconds)<\/span><\/a><\/h3>\n<h3><a href=\"https:\/\/www.practicalclinicalskills.com\/ekg-course-contents?courseid=301\" target=\"_blank\" rel=\"noopener noreferrer\">EKG Online Course Introduction<\/a> <span style=\"color: #000000\">&#8211; Check out the lessons found in the Table of Contents and the EKG Basics Quiz at the bottom of the page.\u00a0 <em>This is not to be used for self diagnosis or diagnosing others.<\/em><\/span><\/h3>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Diversity in Transport\u00a0 (a 2 Week lab unit) &#8211; Online &#8211;working copy?? ?Change format, Pre-Lab, In-Lab, Post Lab [enhanced-heading heading=&#8221;PRE-LAB&#8221; headingtype=&#8221;h5&#8243; headingstyle=&#8221;h2&#8243; target=&#8221;_top&#8221; icon=&#8221;ucomm-heart&#8221; iconstyle=&#8221;circle-filled&#8221; iconposition=&#8221;stacked&#8221; coloroptions=&#8221;text&#8221; textcolor=&#8221;wolfpackred&#8221; fontface=&#8221;universlight&#8221; \/] [insert pre-lab assignment handout] Activity 1: Transpiration in Higher Plants (p. 168-169). Focus on Plant Anatomy and Transpiration\/Translocation &#8211; substitute the potometer procedure with Simulation [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":52,"menu_order":86,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-80","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/80","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/comments?post=80"}],"version-history":[{"count":165,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/80\/revisions"}],"predecessor-version":[{"id":2672,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/80\/revisions\/2672"}],"up":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/pages\/52"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-181l-zchxzbn\/wp-json\/wp\/v2\/media?parent=80"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}