{"id":1339,"date":"2020-04-21T11:36:04","date_gmt":"2020-04-21T15:36:04","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1339"},"modified":"2026-09-04T17:13:15","modified_gmt":"2026-09-04T21:13:15","slug":"unit-2-labs-2-3-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-2-labs-2-3-online\/","title":{"rendered":"Lab Unit 2 Enzyme Lab &#8211; In Person Labs"},"content":{"rendered":"\n<h2 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-1\">Properties of Enzymes &#8211; In Person Lab &#8211; not final yet<\/h2>\n\n\n\n<h4 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\">Scroll below for In-person Labs<\/span><\/h4>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-2-labs-2-3-enzyme-lab-make-up-module\/\" style=\"border-radius:7px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"has-black-color has-text-color has-medium-font-size wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/02\/Cells-and-Enzymes-Handout-Final_2026.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Enzyme Lab Handout (In-Person labs)<\/a><\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">You will need to use <strong>Excel<\/strong> or <strong>Google Sheets<\/strong> to graph your data.&nbsp; As an NCSU student, you can download Microsoft Office 365 from <a href=\"https:\/\/software.ncsu.edu\/software\/microsoft-365-apps-for-enterprise\/\" target=\"_blank\" rel=\"noreferrer noopener\">NC State\u2019s OIT<\/a> software site for free!<\/p>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\">While working along in these Enzyme Experiments, you will want to keep in mind the components of the <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/08\/2013-updated_scientific-method-steps_v6.png\" target=\"_blank\" rel=\"noreferrer noopener\">Scientific Method<\/a> and good experimental design.  This will also serve you well in the 2 assignments that you will work on next week while you are not in lab (Off Lab week).<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Identify the following experimental design terms as you work through the Enzyme Lab experiments this week at your table and\/or throughout the lab room.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Control and Experimental Group(s)<\/li>\n\n\n\n<li>Independent &#8211; what you manipilate or change in the experiment.<\/li>\n\n\n\n<li>Dependent &#8211; what is changed by the experiment, often what is measured.<\/li>\n\n\n\n<li>Controlled Variables &#8211; experimental components that are kept the same.<\/li>\n\n\n\n<li>Replication<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-2\"><span style=\"color: #000000\"><em>Read along in your Enzyme Handout as you work with the information in this webpage.&nbsp; Fill in and answer questions accordingly.&nbsp; <\/em><\/span><strong><span style=\"color: #000000\"><em>Your Enzyme Handout will be collected <\/em><\/span>for grading 24 hours after the end of your lab class.<\/strong><\/h4>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"290\" height=\"174\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/EnzProperties.jpg\" alt=\"Enzyme Proteries\" class=\"wp-image-95\" \/><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"wp-block-image\">\n<figure class=\"alignleft size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"435\" height=\"306\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/inhibitors.jpg\" alt=\"Effect of Inhibitors\" class=\"wp-image-94\" style=\"width:319px;height:223px\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/inhibitors.jpg 435w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/inhibitors-300x211.jpg 300w\" sizes=\"auto, (max-width: 435px) 100vw, 435px\" \/><\/figure>\n<\/div><\/div>\n<\/div>\n\n\n\n<p class=\"style14 wp-block-paragraph\">&nbsp;&nbsp;&nbsp;<\/p>\n\n\n\n<h3 class=\"wp-block-heading style14\"><a href=\"http:\/\/science.howstuffworks.com\/environmental\/life\/cellular-microscopic\/cell2.htm\">How Cells Work &#8211; Enzymes<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">Chemical Reactions and Activation Energy<\/h3>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"363\" height=\"276\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/ActivationEnergy.png\" alt=\"Graph of Activation Energy\" class=\"wp-image-96\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/ActivationEnergy.png 363w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/ActivationEnergy-300x228.png 300w\" sizes=\"auto, (max-width: 363px) 100vw, 363px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">Catalase<\/h3>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"312\" height=\"236\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/catalase.gif\" alt=\"Catalase structure\" class=\"wp-image-98\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-3\">Animation &#8211; <a href=\"https:\/\/www.youtube.com\/watch?v=d_etkljNZxk\">Enzyme Function and the difference between competitive and non-competitive inhibitors. <\/a>(everyone should watch this, esp. Group 2\/Table 2 students)<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1: Qualitative Study of Catalase&nbsp;<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color:#000000\" class=\"tadv-color\"><span style=\"background-color:#abb8c3\" class=\"tadv-background-color\">2H<sub>2<\/sub>O<sub>2<\/sub> + catalase &#8212;-&gt; 2H<sub>2<\/sub>O + O<sub>2<\/sub> + catalase<\/span><\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:16px\"><span style=\"font-size: 12pt\">Catalase is prepared by blending a potato in cold water, then filtered by straining the mixture through 4 layers of cheesecloth.&nbsp; The catalase solution is then kept cold on ice.&nbsp; Why would filtering and maintaining a cold temperature be important for the catalase solution?<\/span><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Making-catalase-e1588373599886.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"225\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Making-catalase-e1588373599886-300x225.jpg\" alt=\"\" class=\"wp-image-1428\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Making-catalase-e1588373599886-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Making-catalase-e1588373599886.jpg 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Procedure A: Testing the Activity of Catalase&nbsp;<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\" style=\"font-size:16px\"><span style=\"font-size: 12pt\">Follow steps 1-3 and complete Table 1 using the results from your 4 prepared test tubes.&nbsp; The picture below shows the appearance of catalase and boiled catalase used Procedure A.&nbsp; What would account for the difference in appearance of the boiled catalase?&nbsp; Where did the &#8220;chunks&#8221; come from?&nbsp;&nbsp;<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Unboiled-and-boild-catalase-e1588373692241.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1427\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Unboiled-and-boild-catalase-e1588373692241-225x300.jpg\" alt=\"\" width=\"197\" height=\"263\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Unboiled-and-boild-catalase-e1588373692241-225x300.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/05\/Unboiled-and-boild-catalase-e1588373692241.jpg 480w\" sizes=\"auto, (max-width: 197px) 100vw, 197px\" \/><\/a>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Procedure B: Testing the Activity of Catalase Under Six Different Environmental Conditions&nbsp;<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><strong>You will be assigned to work with ONE of the six environmental conditions (Groups) below.&nbsp; Propose a plausible hypothesis for your assigned experiment.&nbsp; The same basic reaction as tube #3 in Procedure A is followed, but treated with an assigned environmental condition.&nbsp; Answer all questions and fill in the Table that corresponds to your <em>assigned<\/em> treatment in your lab manual (Tables 2 &#8211; 7).&nbsp; &nbsp;<\/strong><\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-black-color has-text-color has-link-color has-small-font-size wp-elements-4\"><span style=\"font-size: 12pt\"><strong>Group Assignments for Procedure B<\/strong><\/span><strong>:<\/strong><\/h2>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Group I: Effect of Substrate Concentration on Catalase*<\/td><td>Group II: Effect of Hydroxylamine and Subs. Concentration*<\/td><\/tr><tr><td>Group III: Effect of Salt Concentration on Catalase<\/td><td>Group IV: Effect of the Metal Copper Sulfate<\/td><\/tr><tr><td>Group V: Effects of pH on Catalase<\/td><td>Group VI: Effect of Temperature<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>* To make substrate concentration dilutions for Procedure B Groups I &amp; II:<\/li>\n\n\n\n<li>1.5% H<sub>2<\/sub>O<sub>2<\/sub> = 5 ml 3% H<sub>2<\/sub>O<sub>2<\/sub> + 5 ml H<sub>2<\/sub>O<\/li>\n\n\n\n<li>0.75% H<sub>2<\/sub>O<sub>2<\/sub> = 5 ml 1.5 % H<sub>2<\/sub>O<sub>2<\/sub> + 5 ml H<sub>2<\/sub>O<\/li>\n<\/ul>\n\n\n\n<div style=\"height:22px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 2: Quantitative Study of Catalase&nbsp;<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-cyan-bluish-gray-background-color has-text-color has-background\"><span style=\"color:#000000\" class=\"tadv-color\">2H<sub>2<\/sub>O<sub>2<\/sub> + catalase + guaiacol (colorless) &#8212;-&gt; 2H<sub>2<\/sub>O + catalase + tetraguaiacol (orange-brown)<\/span><\/h3>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><span style=\"font-size: 12pt\">We can study the same reaction as above in a more detailed manner by fine tuning the experiment.&nbsp; Instead of learning about the relative activity of catalase, observing more or less reaction, we can introduce a sensitive color indicator to detect the presence of one of the reaction products.&nbsp; The color indicator guaiacol will change from clear to orange-brown as it binds to the O<sub>2<\/sub> produced in this reaction forming tetraguaiacol.&nbsp; This color change can be measured using a spectrophotometer.&nbsp; These data can then be graphed and rates determined for the baseline reaction of catalase, but also for the various treatments.<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Procedure C: Using a Spectrophotometer to Monitor Baseline Catalase Activity&nbsp;<\/span><\/h3>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"640\" height=\"480\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7154.jpg\" alt=\"Spectrophotometer and cuvettes used in the Enzyme lab procedures C and D.\" class=\"wp-image-5218\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7154.jpg 640w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7154-300x225.jpg 300w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><figcaption class=\"wp-element-caption\">Spectrophotometer with cuvettes<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"640\" height=\"480\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7161.jpg\" alt=\"Procedure C Blank and Enzyme Samples. The blank is clear. At time = 0 the sample is slightly colored. At time = 5 minutes the sample is an orange brown color.\" class=\"wp-image-5217\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7161.jpg 640w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7161-300x225.jpg 300w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><figcaption class=\"wp-element-caption\">Procedure C with the blank, initial and final (t=5 minutes) sample test tubes.<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"640\" height=\"480\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7166.jpg\" alt=\"Open spectrophotometer with the time = 5 minute sample cuvette showing the display screen Absorbance = 0.479.\" class=\"wp-image-5216\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7166.jpg 640w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/09\/IMG_7166-300x225.jpg 300w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><figcaption class=\"wp-element-caption\">Open spectrophotometer with the time = 5 minute sample cuvette showing the display Absorbance screen and data.<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><span style=\"font-size: 12pt\"><span style=\"color: #000000;font-size: 12pt\">Carefully read the Procedure C background.&nbsp; This will help you fill in and answer Procedure C questions and Table 8 &amp; 9 in within in your lab handout.&nbsp; <\/span>We will use Genesys spectrophotometers and square disposable cuvettes.&nbsp; If you have not used a spectrophotometer before, view the videos below.&nbsp; It is very important to use an appropriate blank for each treatment that is run.&nbsp; The blank will contain all the same components as the treatment tube, minus the enzyme.&nbsp; This way we will know that any change in color is resulting from the activity of the enzyme itself.<\/span>  <\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">Is the Blank tube a control in the experiment?  What is its purpose?  <\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><a class=\"style14\" href=\"https:\/\/www.youtube.com\/watch?v=pxC6F7bK8CU\">How spectrophotometers work<\/a>&nbsp;&#8211; transmittance and absorbance<\/h3>\n\n\n\n<h3 class=\"wp-block-heading style14\"><a href=\"https:\/\/www.youtube.com\/watch?v=C-M7EtSuD40\">How to use a Genesys 10 Spectrophotometer<\/a><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><span style=\"color: #000000\"><strong>Determining&nbsp; Baseline data<\/strong><\/span> &#8211; Follow directions in Procedure C to collect quantitative data.&nbsp; Then create a graph monitoring the baseline catalase reaction.&nbsp; Be sure to label the graph correctly with all appropriate graphing components outlined by your TA and indicated in the Resources <\/span>links below.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Add a trendline and the equation of the trendline to your graph.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading style14 has-black-color has-text-color\">Graphing Resources:<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><strong>Graphing with Excel<\/strong> &#8211; <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/08\/Aooendix-D-Excel-Mac2018.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Appendix D<\/a> listed on the <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/resources\/\" target=\"_blank\" rel=\"noopener noreferrer\">Resources<\/a> page (Appendix Resources for BIO 181 and 183).&nbsp; This lists the 5 key criteria for a proper graph (p. 204)&nbsp;and also shows how to <strong><em>add a trendline<\/em> and <em>equation for the best fit line<\/em><\/strong> (p. 203).&nbsp; These graph additions are both important skills needed for the Enzyme lab assignment and later lab units and assignments in BIO 183<\/span>. <\/p>\n\n\n\n<p class=\"has-black-color has-text-color has-link-color wp-elements-5 wp-block-paragraph\">Reminder &#8212; NCSU Student can download Microsoft Office 365 from&nbsp;<a href=\"https:\/\/software.ncsu.edu\/software\/microsoft-365-apps-for-enterprise\/\" target=\"_blank\" rel=\"noreferrer noopener\">NC State\u2019s OIT<\/a>&nbsp;&nbsp;software site) for free.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><strong>Understanding and Representing Data<\/strong> \u2013 Appendix E listed on the <a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/resources\/\" target=\"_blank\">Resources<\/a> page (Appendix Resources for BIO 181 and 183).<\/span><\/p>\n\n\n\n<div style=\"height:22px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\">Procedure D: Using a Spectrophotometer to Monitor the Activity of Catalase Under Different Environmental Factors.&nbsp;<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Since you have already learned about the relative effect of ONE environmental condition on catalase, you will next further explore and quantify the same condition you already studied in Procedure B in Procedure D.&nbsp;<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Groups should write their hypothesis about the effect of their assigned treatment on catalase activity.<\/li>\n\n\n\n<li>Design their own procedure to quantify their assigned treatment by carrying out 3-4 different additional trial runs.&nbsp; &nbsp;&nbsp;<\/li>\n\n\n\n<li><strong>Be sure to refer to Hints at the end of Procedure D<\/strong> in your lab handout. (<strong>very helpful!<\/strong>)<\/li>\n\n\n\n<li>While the same basic procedure may be followed, what is needed for the blank tube used in the spectrophotometer?&nbsp; Would the blank be the same as Procedure C? Or does it need to change?&nbsp; Why or why not?<\/li>\n<\/ul>\n\n\n\n<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                        <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Completed Enzyme Lab Unit 2 Lab Handout (all pages) due in Moodle 24 hours after lab<\/P>\n\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Be sure to answer all questions found throughout the labs and the end, and insert your completed Enzyme graph with all group data on one graph.  The graph should include a trendline and line equations for each of the baseline and treatments data.  <br><br>Any images in your Word Document should be saved as JGPs. HEIF iPhone images may appear blank in Moodle for your TA (so can\u2019t be graded).<\/span>\n\t\t\t\t\t\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:23px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading has-black-color has-text-color\"><strong>Off Lab Week after the Enzyme Lab<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">During the off week that follows your Enzymes lab, you will be asked to work on 2 separate assignments.&nbsp; The first assignment (on which you should work individually) is the Experimental Design Assignment.&nbsp; This worksheet will familiarize you with some of the basic requirements of experimental design.&nbsp; This will prepare you to work as a group for the second assignment (Proposed Experiment to Test Enzyme Function).&nbsp; Your answers to both should be written in your own words (not copied or AI generated).&nbsp; Both documents should be submitted on Moodle by Friday 11:59PM of your off week (See due date on Moodle and linked to your Group # 1 or 2 on the <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/bio-183-lab-schedule-fall-2026\/\">Fall 2026 Lab Schedule<\/a>).<\/p>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/01\/Part-1-Exper-Design-Ind.Worksheet_B.docx\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Experimental Design Assignment &#8211; Individual<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">This assignment is worth 10 points. You can type your answers in a document separate from the many pages of the assignment. Be sure to clearly number each answer. This is due by Friday 11:59PM during the Off Week after your Enzyme Lab Week. <br><br>Click this box to view and download the assignment.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-black n_fill-black n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<p class=\"has-small-font-size wp-block-paragraph\">PDF Version of the above <a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/02\/Exper-Design-Assign1-BIO-183_BL-3.pdf\" target=\"_blank\">Take Home Experimental Design Assignment<\/a> in case you cannot see the graphics.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Complete the Experimental Design Assignment above on your own before working as a group on the Enzyme Assignment below.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"> <strong>Since you have already learned about the relative effect of ONE environmental condition on catalase, you will next further explore catalase using the in lab <strong>quantitative method<\/strong> (Procedures C &amp; D) under <strong><em>a different condition<\/em><\/strong> than what your group already studied in Procedure B.<\/strong><\/p>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left is-style-default\" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2025\/09\/Off-Lab-Week-Catalase-ExperDesign-Assignment-rev.docx\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Catalase- Designing Further Experiments &#8211; Group Work<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">This assignment will help you combine the key components of the Experimental Design Assignment above, and the knowledge gained from lab to further our studies of the properties of Catalase. This is due by Friday 11:59PM during the Off Week after your Enzyme Lab Week.<br><br>Click this box to view and download the assignment.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-black n_fill-black n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:22px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2024\/01\/Peer-Evaluation-Form-for-Off-Week-Group-Assign_Sp24.docx\" target=\"_blank\">Group Work Peer Evaluation form(opens in new window)<\/a>&nbsp;<\/strong>&nbsp;\u2013 each should should complete their own peer review and submit it on Moodle by the same due date as the Catalase Designing Experiment Assignment above. <\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-flame   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-white\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-1-final-projects\/\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-white\">Preliminary Pathogen Paper Outline<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-white n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Each group should do all work and have a preliminary outline of their entire paper in each group project Google folder by the start of your Photosynthesis lab.  This outline should build upon your proposal with added text and research from each group member.  <br><br>Use the  Writing a Scientific Review Article provided on the linked Pathogen Group Projects webpage.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-white n_fill-white n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:22px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Properties of Enzymes &#8211; In Person Lab &#8211; not final yet Scroll below for In-person Labs Enzyme Lab Handout (In-Person labs) You will need to use Excel or Google Sheets to graph your data.&nbsp; As an NCSU student, you can download Microsoft Office 365 from NC State\u2019s OIT software site for free! While working along [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":28,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1339","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/comments?post=1339"}],"version-history":[{"count":124,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1339\/revisions"}],"predecessor-version":[{"id":5221,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1339\/revisions\/5221"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/media?parent=1339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}