{"id":1545,"date":"2020-05-08T13:36:50","date_gmt":"2020-05-08T17:36:50","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1545"},"modified":"2026-10-08T10:49:53","modified_gmt":"2026-10-08T14:49:53","slug":"unit-4-lab-1-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-4-lab-1-online\/","title":{"rendered":"Lab Unit 4: Lab 1 DNA Fingerprinting – In Person"},"content":{"rendered":"\n

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\"DNA<\/a><\/h2>\n\n\n\n

DNA Fingerprinting – In Person<\/span><\/h2>\n\n\n\n

Scroll below for In Person Lab unit<\/span><\/span><\/p>\n\n\n\n

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Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n

Read along in your lab handout with the information in this webpage.  Fill in and answer all questions accordingly in the handout.  This handout will be collected and graded later.<\/em><\/span><\/h3>\n\n\n\n

Lab handout for DNA Fingerprinting<\/a> – In-Person<\/span><\/strong><\/p>\n\n\n\n

Important Background Information to review:<\/span><\/h3>\n\n\n\n

An Introduction to the Human Genome<\/a><\/span> – Basic Structure and Variation in Human DNA (5:32)<\/span><\/p>\n\n\n\n

Restriction Enzymes and how they are used with DNA<\/a> <\/span>– show how DNA with Restriction Enzymes\/Endonucleases and how strands can be put back together with DNA Ligase (another enzyme). Click on the Transcript tab if needed.<\/p>\n\n\n\n

Polymerase Chain Reaction<\/a> (PCR) 3D Animation Cold Springs Harbor  \u2013 click on video – used to make larger samples of DNA from an initial small sample of DNA. Click on the Transcript tab if needed.<\/p>\n\n\n\n

PCR Animation<\/a>  \u2013 a nice simple step by step animation to show how PCR works.<\/p>\n\n\n\n

DNA Fingerprinting using PCR<\/a> to isolate and amplify Short Tandem Repeats (STR) in small DNA samples, then what they look like separated by DNA Electrophoresis. (4:09)<\/p>\n\n\n\n

DNA Gel with labeled known DNA fragment sizes\/DNA Ladder\/Marker DNA (lane 1). This also shows the different banding pattern results (different sized fragments) produced when using different restriction enzymes on the same DNA.<\/span><\/p>\n\n\n\n

\"DNA<\/a><\/figure>\n\n\n\n

DNA Electrophoresis Equipment:<\/span><\/h3>\n\n\n\n

How DNA Electrophoresis works<\/a> – the electrophoretic process.<\/strong><\/p>\n\n\n\n

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\"DNA<\/figure>\n<\/div>\n\n\n\n
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\"Micropipette<\/figure>\n<\/div>\n<\/div>\n\n\n\n
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Activity 1; Practice Pipetting – short videoclips<\/span><\/h3>\n\n\n\n