{"id":1548,"date":"2020-05-08T13:44:39","date_gmt":"2020-05-08T17:44:39","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1548"},"modified":"2026-07-27T19:02:53","modified_gmt":"2026-07-27T23:02:53","slug":"unit-4-labs-2-3-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-4-labs-2-3-online\/","title":{"rendered":"Unit 4: Labs 2 &amp; 3 Gene Transformation \u2013 In Person"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/GFP-jellyfish.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" class=\"alignnone size-thumbnail wp-image-66\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/GFP-jellyfish-150x150.jpg\" alt=\"Glowing jellyfish\"><\/a><\/h2>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Gene Transformation &#8211; In Person &#8211; Spring 2023 (Not currently done in BIO 183 labs)<\/h2>\n\n\n\n<p class=\"has-pale-pink-color has-text-color wp-block-paragraph\">Scroll below for In Person Lab unit &#8211; <em>Work in Progress, parts may support online learning &amp; assignments<\/em><\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-4-labs-2-3-gene-transformation-make-up-module\/\" style=\"border-radius:8px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/11\/Gene-TransformationWk1_Fall21_InPersonOnline.docx\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Lab Handout for Gene Transformation lab &#8211; Part 1<\/span><\/a><\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Read along in your Part 1 and Part 2 (see below) lab handouts with the information on this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; These will be collected and graded later.<\/em><\/span><\/h4>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Parts 1 and 2 will cover 2 weeks of lab.<\/em><\/span><\/h4>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/images.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"225\" height=\"224\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/images.jpg\" alt=\"pGlo Plasmid DNA\" class=\"wp-image-312\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/images.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/images-150x150.jpg 150w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/pGLO_Circle.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"457\" height=\"154\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/pGLO_Circle.jpg\" alt=\"pGlo plasmid and transformed bacterial culture\" class=\"wp-image-313\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/pGLO_Circle.jpg 457w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/pGLO_Circle-300x101.jpg 300w\" sizes=\"auto, (max-width: 457px) 100vw, 457px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading style11\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Good background information.&nbsp; Also read the Additional Background Information in your lab handout.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.youtube.com\/watch?v=Ik_Pxht1LM0\">Using Restriction Enzymes and DNA Ligase to make plasmids<\/a>&nbsp;<span style=\"color: #000000\">(1:38)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"https:\/\/www.youtube.com\/watch?v=_HdMkSEQU8k\">Stanford Scientists Produce Cancer Drugs from Rare Plants<\/a>&nbsp;<span style=\"color: #000000\">(1:59) <span style=\"font-size: 14pt\">by making plasmids and inserting them into more easily grown plants or yeasts.<\/span><\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"http:\/\/www.youtube.com\/watch?v=GmhD-RWNL6c&amp;sns=fb\">Funding Basic Science to Revolutionize Medicine<\/a>&nbsp;<span style=\"color: #000000\">(3:59) <span style=\"font-size: 14pt\">that show how the focus in research has changed over the past 50 years.<\/span><\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a class=\"style11\" href=\"http:\/\/www.youtube.com\/watch?v=Sl2PRHGpYuU&amp;feature=related\">How GFP is used in research<\/a><span class=\"style11\">&nbsp;<span style=\"color: #000000\">(10:03)<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.youtube.com\/watch?v=c40UudFIlGw&amp;t=2s\" target=\"_blank\" rel=\"noopener noreferrer\">Video of BIORAD pGlo Transformation procedure<\/a><span style=\"color: #000000\"> (7:01) <span style=\"font-size: 14pt\">&#8211; what we will do in lab.&nbsp;<\/span><\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/10\/Bacterial-chromosome.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"141\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/10\/Bacterial-chromosome-300x141.jpg\" alt=\"\" class=\"wp-image-1949\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/10\/Bacterial-chromosome-300x141.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/10\/Bacterial-chromosome.jpg 320w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1: In-Lab discussion with your TA &#8211; Important Concepts in the Transformation Procedures:<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/pGlo-Plasmid.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">pGlo Plasmid<\/a> <span style=\"color: #000000;font-size: 14pt\">we will use in lab.&nbsp; Why are these genes important?&nbsp; What do they code for?<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/transformation2.html\">DNA transformation using plasmids<\/a>&nbsp;<span style=\"color: #000000;font-size: 14pt\">&#8211; animation explaining the steps in our Transformation procedure.&nbsp; It is very important to understand the nature of the bacterial cell wall, why CaCl<sub>2<\/sub> is used, and why the heat shock is important.<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Answer the Protocol Discussion questions #1-8.<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Measurement marks on disposable plastic pipettes provided in the BIORAD kit:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/1-ml-pipet-diagram.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"540\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/1-ml-pipet-diagram.jpg\" alt=\"pipet measurements\" class=\"wp-image-314\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/1-ml-pipet-diagram.jpg 720w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/1-ml-pipet-diagram-300x225.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3 &#8211; Experimental Treatments and Proposed Results<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #000000;font-size: 12pt\">Fill out the table at the end of this handout to indicate what you think the results would be for each of the 4 agar plates that were inoculated in this procedure.&nbsp; <strong>Also<\/strong> fill out the gray boxes for the agar plates that were not inoculated, but still indicate what you&#8217;d expect to happen with those nutrient conditions for the bacterial growth plates that did and did not get the plasmid added.&nbsp; Include why you think you&#8217;d get these results in ALL the boxes as well.<\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">End of Transformation Part 1 (week 1)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\">Start of Transformation Part 2 (week 2).<\/span><\/h3>\n\n\n\n<h2 class=\"wp-block-heading\"><span style=\"color: #000000\">Transformation Efficiency Calculation<\/span><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/10\/Transformation-Wk2-Efficiency-Calculations-InPerson.docx\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Lab Handout for Gene Transformation Calculations &#8211; Part 2 In Person lab<\/span><\/a><span style=\"font-size: 12pt;color: #000000\"> &#8211; read before coming to lab.&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1: Data Collection from Transformation Plates<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Your Results:&nbsp; Record your bacterial growth on the different nutrient agar plates under white light and UV light.<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">Record your data in Table 1 in your lab handout that corresponds to your assigned Group.&nbsp; Be sure to collect data under both White Light and the UV Light.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Answer questions #2-6.&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000;font-family: UniversCondensed, Arial, sans-serif;font-size: 21px;font-weight: bold\">Activity 2: Calculating Transformation Efficiency<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">Read through the provided information and follow the prompts to calculate the transformation efficiency on your assigned <strong>+LB\/amp\/ara agar plate.<\/strong>&nbsp; <em>Be sure<\/em> to include the <em>units<\/em> as you set up each of the equations.&nbsp; &nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Transformation Efficiency data from each lab group will be recorded and shared on the class whiteboard.&nbsp; <\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Answer all the Discussion Questions at the end of the lab unit.&nbsp;&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=plVk4NVIUh8&amp;t=14s\" target=\"_blank\" rel=\"noopener noreferrer\">The Evolution of Bacteria on a Mega-Plate Petri Dish<\/a> &#8211; Natural mutation for antibiotic resistance in bacteria growing on nutrient agar containing an antibiotic.<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading style10\"><a href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\">Molecular Visualization of DNA&nbsp;<\/a><\/h3>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">0-1:48 = DNA Structure &#8212; Also seen in the Mitosis and Meiosis lab<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">1:48-2:53 = DNA Replication &#8211;Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">2:53-4:48 = Transcription &#8211; (you will learn about this and Translation later in lecture)<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\"><span style=\"color: #000000\">4:48-6:57 = Translation &#8211; <\/span><span style=\"color: #000000\">(you will learn about this and Translation later in lecture)<\/span><\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                        <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">DNA Presentations will be presented live in lab using PowerPoint or Google Slides June 15 in Lab.<\/P>\n\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \"><br>To practice your presentations skills, each group of students will receive a DNA Topic and Target Audience to present to the class. These presentations should run 5-10 minutes. Each individual will need to speak and be equal partners in the presentation. All presentations will be recorded so that a self assessment can be done by each students of their own presentation skills. See the links below for more information. This will help you better organize and present your Group Review Paper Presentations the final week of labs.<br><br>Each student will use the Peer Evaluation form provided below to evaluate themselves and each person in their group. Submit this on Moodle by the start of the lab the week of your presentation.<\/span>\n\t\t\t\t\t\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<h3 class=\"wp-block-heading style9\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/03\/BIO-183-DNA-Pres-ExpectationsSelf-Assess-Sp2023.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation Expectations and Self Assessment<\/a> <span style=\"color: #000000\">&#8211; 30 points total<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading style9\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/03\/DNAPresEvalSheetTA_2023docx.docx\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation TA Evaluation Sheet<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/10\/Peer-Evaluation-Form-for-DNA-Presentations.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Peer Evaluation form for DNA Group Presentations<\/a><\/h3>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong>Additional Information on Biotechnology<\/strong>:<\/p>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000\">Check out the opportunities for undergraduate students! Certificate, Minor, Courses&#8230;..(look under Academic Programs)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Link to information on a Minor in the<\/span> <a rel=\"noopener noreferrer\" href=\"https:\/\/biotech.ncsu.edu\/minors-certificate\/\" target=\"_blank\">Biotechnology Program at NCSU<\/a>&nbsp;<span style=\"color: #000000\">and<\/span>&nbsp;<a href=\"https:\/\/biotech.ncsu.edu\/spring-2023-2\/\" target=\"_blank\" rel=\"noreferrer noopener\">Courses in Biotechnology (BIT) at NCSU<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000\">Link to the<\/span> <a href=\"https:\/\/www.btec.ncsu.edu\/academic\/undergraduate\/index.php\" target=\"_blank\" rel=\"noopener noreferrer\">Undergraduate Programs at the BTEC center on Centennial Campus NCSU<\/a>&nbsp;<span style=\"color: #000000\">&#8211; where industry and education meet.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000;font-size: 14pt\">At BTEC they use similar transformation procedures to engineer bacteria and yeasts to make (biomanufacturing) chemicals, medicines, vaccines, enzymes, proteins, biofuels&#8230;&#8230;.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Careers in Biotech &#8211; <a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noopener noreferrer\">nice website.&nbsp; <\/a>Careers&nbsp;at the Bench and Beyond<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000\">Great Short articles related to increasingly important Biotechnology&nbsp;<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.conncoll.edu\/ccacad\/zimmer\/GFP-ww\/cooluses0.html\" target=\"_blank\" rel=\"noopener noreferrer\">GFP &#8211; Green Fluorescent Protein<\/a> &#8211; Cool Uses<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.genetics.org\/content\/199\/2\/293\" target=\"_blank\" rel=\"noopener noreferrer\">Brainbow<\/a> &#8211; New Resources and Emerging Biological Applications for Multicolor Genetic Labeling and Analysis &#8211; Informative and beautiful images!<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">PCR Animation<\/span><\/a><span style=\"font-size: 12pt\"> &#8211; a nice simple step by step animation to show how PCR works.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a> &#8211; animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/11\/Biology_of_SARS-CoV-2-1.pdf\" target=\"_blank\">Biology of SARS-CoV-2<\/a> &#8211; nice powerpoint with basic explanations<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/07\/Ten-Years-of-CRISPR.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Ten Years of CRISPR<\/a> &#8211; highlights 7 ways that CRISPR has been used in gene editing<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Plasmids, Horizontal Gene Transfer and Anitmicrobial Resistance &#8211; <a href=\"https:\/\/news.ncsu.edu\/2026\/07\/plasmids-horizontal-gene-transfer-and-antimicrobial-resistance\/\">short NCSU news release summary<\/a> and <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/07\/Plasmid-Mediated-antimicrobial-resistance.pdf\">published paper<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Gene Transformation &#8211; In Person &#8211; Spring 2023 (Not currently done in BIO 183 labs) Scroll below for In Person Lab unit &#8211; Work in Progress, parts may support online learning &amp; assignments Lab Handout for Gene Transformation lab &#8211; Part 1 Read along in your Part 1 and Part 2 (see below) lab handouts [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":16,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1548","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1548","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/comments?post=1548"}],"version-history":[{"count":6,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1548\/revisions"}],"predecessor-version":[{"id":5188,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/1548\/revisions\/5188"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/media?parent=1548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}