{"id":198,"date":"2018-06-13T14:39:51","date_gmt":"2018-06-13T18:39:51","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=198"},"modified":"2026-08-25T05:26:07","modified_gmt":"2026-08-25T09:26:07","slug":"unit-2-lab-2-1","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-2-lab-2-1\/","title":{"rendered":"Lab Unit 1 Microscopes and Cells"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">&nbsp;<a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/cheek_cells1.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" class=\"alignnone size-thumbnail wp-image-63\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/cheek_cells1-150x150.jpg\" alt=\"Cheek Cells\"><\/a><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\">Microscopes and Cells<\/span><\/h2>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-2lab-1-online-lab\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n<p class=\"has-black-color has-text-color has-medium-font-size wp-block-paragraph\"><span style=\"color: #000000\"><strong><span style=\"font-size: 12pt\">Scroll below for In Person lab information.&nbsp;<\/span><\/strong><\/span><\/p>\n\n\n\n<h2 class=\"wp-block-heading has-black-color has-text-color\"><em>For all In Person BIO 183 lab units &#8211; Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; Your lab handout will be collected and graded later.<\/em><\/h2>\n\n\n\n<p class=\"has-black-color has-text-color has-medium-font-size wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/01\/183-Lab-Policies-2023.pdf\" target=\"_blank\">Lab Policies<\/a>&nbsp;<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/01\/Safety-Guidelines.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Lab Safety Guidelines<\/a><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2026\/01\/Handout-Microscopes-and-cells-2026.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Microscopes and Cells Lab Handout<\/a> <\/strong> &#8211; Includes areas for your labeled drawings and answering questions. Please bring a paper copy of the Lab Handout to lab OR bring your laptop\/tablet and paper and pen\/pencils to make your drawings in lab. They will later need to be inserted as jpg or pdf images in your Lab Handout.<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><strong><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/02\/Covid-19-Microscope-Cleaning.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">COVID_19 Safety Precautions for Compound Microscope Use<\/a><\/strong> &#8211; directions and supplies will be provided at each student table for use in the Laboratory.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">The Compound Light Microscope<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"500\" height=\"469\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cm501microscopediagram.jpg\" alt=\"microscope with labeled parts\" class=\"wp-image-187\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cm501microscopediagram.jpg 500w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cm501microscopediagram-300x281.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000\">Helpful Links<\/span><\/h3>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"http:\/\/www.biologycorner.com\/worksheets\/microscope_use.html\" target=\"_blank\" rel=\"noopener noreferrer\">Microscope Key Points, Diagrams, and Quizzes<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=SUo2fHZaZCU\" target=\"_blank\" rel=\"noopener noreferrer\">How to use the microscope video<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a><\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"https:\/\/www.ncbionetwork.org\/iet\/microscope\/\" target=\"_blank\" rel=\"noreferrer noopener\">The&nbsp;<span class=\"screen-reader-text\">(opens in new window)<\/span>Virtual Microscope<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>&nbsp;&nbsp;developed by the NC Community Colleges BioNetwork<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/AppendixA_micro08.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Appendix A<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>&nbsp;of BIO 181\/183 Lab Manuals \u2013 Use and Care of Microscopes<\/p>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><a href=\"http:\/\/micro.magnet.fsu.edu\/primer\/java\/scienceopticsu\/powersof10\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">Exploring Magnification: Power of Ten video<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading style11 has-black-color has-text-color has-link-color wp-elements-1\">Microscope use reminders<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Always start by using the 4X objective lens<\/strong>. Find the specimen and focus using the coarse focus, then the fine focus knob.&nbsp;<strong>Only use the coarse focus know with the 4X objective lens<\/strong>.&nbsp; Then progress to the 10X objective lens, then 40X lens and oil\/100X lenses. Be sure to focus at EACH&nbsp;<strong>new objective lens (10X, 40X, 100x) using ONLY the fine focus knob<\/strong>. Do not skip using a lens. Always progress one lens at a time.<\/li>\n\n\n\n<li><strong>Label all drawings<\/strong>&nbsp;with organisms name and indication of size: total magnification, field of view, size bar, or lens used in relation to the field of view.&nbsp; Total magnification = object lens x ocular lens.<\/li>\n\n\n\n<li>Other information: date, time, place and any relevant notes.<\/li>\n\n\n\n<li>Record all observations; don\u2019t rely on memory.<\/li>\n\n\n\n<li>Make sure to record what you SEE, not what you THINK you should see.<\/li>\n\n\n\n<li>Detail is key, pay attention to even the smallest things.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading style11\"><span style=\"color: #000000\">Activity 1:&nbsp; Procedure 1A&nbsp;&nbsp;Practice Use of the Compound Microscope Using a Prepared Slide<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Review the names and use of all the major parts of the microscope and use of lens paper needed for microscopy.&nbsp; This light microscope has a 10X&nbsp;<strong>ocular<\/strong>&nbsp;lens and 4 different&nbsp;<strong>objective<\/strong>&nbsp;lenses (4X, 10X, 40X and 100x\/oil lens), though we will not use the 100X oil lenses in our labs.<\/li>\n\n\n\n<li>Select ONE of the prepared slides found at your table.&nbsp; Use the course focus to find the specimen with the 4X lens.&nbsp; First use the Course Focus, then the Fine Focus adjustors to see the best image of the specimen.&nbsp; You may need to adjust the level of light intensity and\/or iris diaphragm to most clearly see the specimen.&nbsp; Continue to increase magnification until you can best see the cells.<\/li>\n\n\n\n<li>Draw a few of the cells in the indicated circles in your lab manual.&nbsp; Keep in mind to draw the cell size in relation to the field of view.&nbsp; Indicate the name of the slide, total magnification you thought was most appropriate, and any structures you can discern.&nbsp; You can search the internet to help with labeling 2 structures within the image you choose to draw,<\/li>\n\n\n\n<li>Answer the questions found in the lab handout\/Lab Unit write up.<\/li>\n\n\n\n<li><strong>Class Discussion<\/strong>&nbsp;\u2013 Below are images of bacteria, animal, plant and fungal cells.&nbsp; What visual clues can you see to help you determine if they are bacterial, animal, plant or fungal cells?&nbsp; Think back to BIO 181 labs or characteristics of cells belonging in different Kingdoms.&nbsp; What information is useful?&nbsp; What else would you need to know when looking under a light microscope?<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\">Follow the directions in the lab handout\/write up<\/span><\/h4>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>1.&nbsp;<img decoding=\"async\" class=\"alignnone wp-image-189\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell1-150x150.jpg\" alt=\"Cell 1, irregular thick cell walls with lip shaped structures found within the cell layer.\"><\/td><td>2.&nbsp;<img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" class=\"alignnone wp-image-190\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell2-300x300.jpg\" alt=\"Cell 2, large irregular shaped cell with a small dark central structure, with long thin armlike sprojections.\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell2-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell2-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell2.jpg 600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td><td>3.&nbsp;<img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" class=\"alignnone wp-image-191\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell4-e1528914451404-300x300.jpg\" alt=\"Cell 4, irregular thick walled oval cell containing smaller green structures.\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell4-e1528914451404-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell4-e1528914451404-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Cell4-e1528914451404.jpg 360w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td><\/tr><tr><td>4.&nbsp; <img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" class=\"alignnone wp-image-192\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/bact1-e1528914586726-300x300.jpg\" alt=\"cell 4, very small log shaped cells.\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/bact1-e1528914586726-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/bact1-e1528914586726-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/bact1-e1528914586726.jpg 324w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td><td>5. <img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" class=\"alignnone wp-image-193\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669-300x300.jpg\" alt=\"cell 5, many small irregularly shaped cells, some with smaller dark staining structures.  \" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669-768x768.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669-1024x1024.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/cell3B-e1528914769669.jpg 1535w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td><td>6.&nbsp;<img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" class=\"alignnone wp-image-194\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/fungus-e1528914852251-300x300.jpg\" alt=\"cells 6, long tubular cells with occasional smaller round clusters of dark staining cells.\"><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1:&nbsp; Procedure 1B Simple Staining with Animal and Plant Cells (Eukaryotic Cells)<\/span><\/h3>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\">Simple staining is the addition of ONE type to stain that can allow you to better see cells and\/or structures under the light microscope.<\/p>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\">In lab you will make your own slides of cheek and onions cell slides.&nbsp; On their own, these cells are relatively clear so we will use Toluidine Blue stain to better visualize the them.&nbsp; You will need to view the cells at low, medium, and high power (4X, 10X, and 40X objective lenses) to determine the most appropriate magnification for each of these cells.<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Students will work in pairs, with one student making the cheek cell slide and the other making the onion cell slide.  The other pair of students at the table should ALSO be making these same slide so that everyone builds their microscope and observational skills.<\/li>\n<\/ul>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\">If you are wearing a mask and are uncomfortable removing it to sample your cheek cells in the lab, you can go <strong>OUTSIDE<\/strong>&nbsp;on the breezeway with a toothpick to collect your own cheek cells.&nbsp; <\/p>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\">How would you describe the animal cells?&nbsp; The plant cells? Size, shape organization? What&nbsp;<strong>structures and organelles<\/strong>&nbsp;can you see that help you identify the type\/kingdom of these cells? What cellular organelles can\u2019t you see? Why can\u2019t you see them?&nbsp; What is the best size to view these animal and plant cells?<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 2: Procedure 2A Osmosis in Plant Cells (<em>Elodea<\/em>)<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Decide which magnification is most appropriate and draw both in the appropriate location in your lab handout.<\/li>\n\n\n\n<li>Be sure to label the structures that you can see in each these specimen.&nbsp; At a minimum, you should be able to label 2 structures found in these cells.<\/li>\n\n\n\n<li>Based in these slides, how are these cells similar?&nbsp; How are they different?<\/li>\n<\/ul>\n\n\n\n<p class=\"has-medium-font-size wp-block-paragraph\"><em>Elodea<\/em>&nbsp;is an aquatic plant that provides habitat and oxygen for many organisms in freshwater lakes, ponds and low flow areas in streams.&nbsp; It is commonly used in freshwater fishtanks and biology labs!<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">a.&nbsp;&nbsp;<a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/02\/Elodea_whole.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"225\" class=\"alignnone size-medium wp-image-2875\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/02\/Elodea_whole-300x225.jpg\" alt=\"Low magnification view of Elodea plant growing in water.\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/02\/Elodea_whole-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/02\/Elodea_whole-768x576.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2022\/02\/Elodea_whole.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>. b.&nbsp;&nbsp;<a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113.jpeg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"225\" class=\"alignnone wp-image-1779 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113-300x225.jpeg\" alt=\"Low and higher magnification view of Elodea plant growing in water.\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/08\/Image-113.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Each pair of students will need to obtain 1 whole leaf of&nbsp;<em>Elodea<\/em>&nbsp;off the main stems from the side counter culture (a).&nbsp; Then make a wet mount slide taking care not to introduce air bubbles under your coverslip.&nbsp; Above in \u201cb\u201d you can see a whole leaf mounted using freshwater at 40X total magnification.&nbsp; What structures can you see here? \u2026. not much!&nbsp; Higher magnification is needed to clearly see whole cells.<\/li>\n\n\n\n<li>Draw the&nbsp;<em>Elodea<\/em>&nbsp;cells at the appropriate magnification in the leftmost circle in your lab handout.&nbsp; Label all the structures you can see.<\/li>\n\n\n\n<li>Your TA will assign each pair of students either the 5% and 10% Saline (NaCl) treatment to flush the leaves with different concentrations of saline.<\/li>\n\n\n\n<li>Draw your saline flushed cells.&nbsp; Label all the structures you can see.&nbsp; Make note of any changes that you see.<\/li>\n\n\n\n<li>Then flush the cells with freshwater (dH<sub>2<\/sub>0) and draw the cells.&nbsp; Once again label all the structure you can see and make note of any changes that you see.<\/li>\n\n\n\n<li>Answer the questions 7-9 in your lab handout.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:31px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\">Activity 2: Procedure 2B Osmosis in Blood Cells<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"602\" height=\"461\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/02\/SEM-of-Blood-cells.jpg\" alt=\"\" class=\"wp-image-3556\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/02\/SEM-of-Blood-cells.jpg 602w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/02\/SEM-of-Blood-cells-300x230.jpg 300w\" sizes=\"auto, (max-width: 602px) 100vw, 602px\" \/><figcaption class=\"wp-element-caption\">Scanning Electron Micrograph showing 3 types of blood cells.<\/figcaption><\/figure>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Working in pairs and as a table, follow the directions in your Lab Handout.  <\/li>\n\n\n\n<li>Each pair of students will make one slide to see blood cells in 0.87% Saline and in 5% saline. First add a very small drop of the 0.87% saline on one end of the slide and your TA will add <strong>0.1 ul<\/strong> of blood to that drop. Add a coverslip and observe, then draw what you see.<\/li>\n\n\n\n<li>Then add a very small drop of 5% saline to the other end of the same slide.  Again, your TA will add 2 ul of blood to this drop.  Add a coverslip and observe, then draw what you see.<\/li>\n\n\n\n<li>Lastly, view the blood cells in dH2O. If there is room on your slide for 1 more cell prep, go ahead and add a tiny drop of water, then 0.2 ul of blood cells and a coverslip and quickly view the cell preparation. If needed you use another slide to make this last blood cell preparation.<\/li>\n\n\n\n<li>As a pair, the students should both be viewing these slides and making their own sketches. <\/li>\n\n\n\n<li><\/li>\n\n\n\n<li>Overall, the blood cells should be observed under 3 different environmental conditions: dH2O, 0.87% saline and 5% saline.  <\/li>\n\n\n\n<li>Draw, label and explain what you see under all 3 conditions using the appropriate osmotic terminology: isotonic, hypotonic and hypertonic.  <\/li>\n\n\n\n<li>It may be hard to see the red blood cells once they have been in the dH2O for a while.  Viewing the Osmosis in Red Blood Cells video below may be helpful &#8211; especially starting 2:51.<\/li>\n\n\n\n<li>Also be sure to answer any other written questions.<\/li>\n<\/ul>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading has-black-color has-text-color has-medium-font-size\">Videos of Osmosis in a different plant cell and blood cells:<\/h4>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\"><div class=\"wp-block-image\">\n<figure class=\"alignright size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"260\" height=\"185\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/redOnion.jpg\" alt=\"Red onion cells\" class=\"wp-image-209\" style=\"width:405px;height:288px\" \/><figcaption class=\"wp-element-caption\"><a class=\"style11\" rel=\"noopener noreferrer\" href=\"https:\/\/www.youtube.com\/watch?v=Mp_CJBqRI5A\" target=\"_blank\">Video: Osmosis in Red Onion Epidermis<\/a>&nbsp;&#8211; (1:41) flushing various concentrations of solution through these plant epidermal cells.<\/figcaption><\/figure>\n<\/div><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"768\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/red_blood_cells-1024x768.jpg\" alt=\"red blood cells\" class=\"wp-image-211\" style=\"width:375px;height:282px\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/red_blood_cells-1024x768.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/red_blood_cells-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/red_blood_cells-768x576.jpg 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\"><a class=\"style11\" rel=\"noopener noreferrer\" href=\"https:\/\/www.youtube.com\/watch?v=A8cI6FkcG4c\" target=\"_blank\">Video: Osmosis in Red Blood Cells<\/a>-(3:47) see how red blood cells appear in isotonic, hypertonic and hypotonic solutions.<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:24px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"240\" height=\"187\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/InLifCell.jpg\" alt=\"Inner Life of the Cell video still\" class=\"wp-image-212\" style=\"width:338px;height:263px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3: Animated Film &#8211; The Inner Life of the Cell<\/span><\/h3>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\"><a class=\"style11\" title=\"Inner Life of Cell video\" href=\"https:\/\/www.youtube.com\/watch?v=wJyUtbn0O5Y\" target=\"_blank\" rel=\"noopener noreferrer\">Video: Cellular Visions: Inner Life of the Cell video<\/a>&nbsp;(3:12)- Read the summary below before you watch the video. Click on the link and scroll down and click the play video arrow.<\/p>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\"><strong>Video Background:<\/strong>&nbsp; This video starts with a view of the inside of a blood vessel.&nbsp; You will see the red blood cells rushing past and the much larger white blood cells rolling along the wall of the blood vessel.&nbsp; The white blood cell receives an extracellular signal and the majority of the video shows what happens inside the white blood cell as it responds to that signal.&nbsp; The final scene shows the white blood cell reshaping (due to the conformational changes of the cytoskeleton) and slipping through the endothelial cells of the blood vessel wall to the site of inflammation.<\/p>\n\n\n\n<p class=\"style10 has-medium-font-size wp-block-paragraph\">As you watch the video, how many structures\/organelles can you see? Can you find -protein filaments, lipid rafts, plasmamembrane, actin filaments, microtubules, motor proteins, transport vesicles, mitochondria, centrosomes, nucleus, nuclear pore, mRNA, ribosomes making proteins, ER, golgi apparatus, vesicles transported back to the plasma membrane surface.<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                        <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Completed Microscopes &amp; Cells Unit 1 Lab Handout (all pages) due in Moodle 24 hours after lab <\/P>\n\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Be sure to answer all questions found throughout the lab handout and the end, and insert your labeled sketches in your handout.  Any images in your Word Document should be saved as JGPs.  HEIF\/HEVC iPhone images and items saved as Pages may appear blank in Moodle for your TA (so can&#8217;t be graded).<\/span>\n\t\t\t\t\t\t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2025\/08\/Intro-Biology-Lab-Group-Work-Contract-F25.docx\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Group Work Contract<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Complete the linked Group Work Contract template as a group.  This contract will serve as guidelines for all your  graded group work in lab.  <br>Please complete the template within 48 hours of the in-person lab.  It will help you plan and complete ALL group work due by Friday 11:59PM of the Online Lab weeks and work on your Pathogen Project.<br>Leave a copy of the completed form in your Lab Group Work Google folder. <br> Your TA will review this.  The group can edit it at any time as long as the whole group agrees.  Let your TA know if you make changes.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-black n_fill-black n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-flame   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-white\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-1-final-projects\/\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-white\">Semester Long Pathogen Group Project<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-white n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Click on this box to link to the Pathogen Project Webpage.  Your TA will assign groups this week and a Group Project Proposal with supporting information is due by Friday 11:59PM of the Off Lab Week following the Microscopes and Cells Lab.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-white n_fill-white n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:16px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<H2 id=\"\" class=\"block-icon-heading n_ma0 n_ttn n_relative n_flex n_items-center n_flex-row   n_text-gray-90 n_normal n_f3  n_justify-start\">\n                    \n    \n    \n        <span id=\"\" class=\"block-icon-heading__icon n_mr3 n_fls0 icon n_text-gray-90 n_fill-gray-90 n_br0  n_s2 \" aria-hidden=\"true\">\n    \n\n\n    <svg version=\"1.1\"  xmlns=\"http:\/\/www.w3.org\/2000\/svg\" xmlnsXlink=\"http:\/\/www.w3.org\/1999\/xlink\" x=\"0px\" y=\"0px\" viewBox=\"0 0 50 50\" enableBackground=\"new 0 0 50 50\" xmlSpace=\"preserve\" class=\"n_mw-100 n_w-100 n_h-100\" >\n      <g>\n        <path  d=\"M44.4,10l-10-10L5.5,0v50h39L44.4,10z M35.2,4.2l5.1,5.1h-5.1V4.2z M7.9,47.6V2.4l24.9,0v9.3H42l0.1,35.9\n\tH7.9z\" \/>\n        <rect x=\"12.3\" y=\"7.6\"  width=\"19.4\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"18.7\"  width=\"25.1\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"13.1\"  width=\"25.1\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"24.2\"  width=\"25.1\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"29.8\"  width=\"25.1\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"35.3\"  width=\"25.1\" height=\"1.9\" \/>\n        <rect x=\"12.3\" y=\"40.9\"  width=\"12.7\" height=\"1.9\" \/>\n      <\/g>\n    <\/svg>\n\n<\/span>\n    <span class=\"gray-90\">\n        <span class=\"n_fls0 block-icon-heading__content n_univers-light \">\n            Additional Information\n        <\/span>\n    <\/span>\n<\/H2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=0xe1s65IH0w\" target=\"_blank\" rel=\"noreferrer noopener\">Overview of Cell Structures<\/a> &#8211; nice video with clear explanations of cellular structures, organelles and function (7:28).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Cool Volume Electron Microscopy imaging:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a rel=\"noreferrer noopener\" href=\"https:\/\/players.brightcove.net\/1964492303001\/JwDxEyMho_default\/index.html?videoId=6331345861112&amp;cid=msd_ls_acd_bioc_voled_1382550_gl_0db_sci_ye0xhr&amp;en=msd_ls_xx_em_con_2311_c06795_p1382550_volume-em-cell-bio-html&amp;utm_source=the-scientist&amp;utm_medium=3rd-party&amp;utm_campaign=ls_xmarket_gl_volume-em-_2023&amp;_hsmi=281907610&amp;_hsenc=p2ANqtz-_EPO1nylToGGvNtEhI-R2rZoUeE4B5xfD44aYgKDGojmYnnd1yyuurcik_Q8Xyex2yxSre17fBT1dNfM1uiakBIHvGgg&amp;utm_content=281907610\" target=\"_blank\">3D Reconstruction of Chlamydomonas reinhardtii<\/a> &#8211; short videoclip<\/li>\n\n\n\n<li><a rel=\"noreferrer noopener\" href=\"https:\/\/players.brightcove.net\/1964492303001\/JwDxEyMho_default\/index.html?videoId=6165468136001&amp;cid=msd_ls_acd_bioc_voled_1382550_gl_0db_sci_ye0xhr&amp;en=msd_ls_xx_em_con_2311_c06795_p1382550_volume-em-cell-bio-html&amp;utm_source=the-scientist&amp;utm_medium=3rd-party&amp;utm_campaign=ls_xmarket_gl_volume-em-_2023&amp;_hsmi=281907610&amp;_hsenc=p2ANqtz-9ddxYQaqL2FmD9fQyj3qGklDR1HXXz4OaBcvy5pJKWTwDtlHevypoDzcliPUnCvao-mLraMMmMJ6tSLFUq_yX5lOk2YQ&amp;utm_content=281907610\" target=\"_blank\">3D Reconstruction of a mouse brain<\/a> &#8211; short videoclip<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"http:\/\/news.ncsu.edu\/2013\/08\/tp-adler-cancer\/\" target=\"_blank\" rel=\"noreferrer noopener\">Article: NCSU Researcher \u201cStopping Cancer in its Track\u201d(opens in new window)<\/a>&nbsp;&nbsp;\u2013 Examines the role to which the cytoskeleton\/actin is involved in movement of cancer cells.&nbsp; Remember \u201cmoving through\u201d the cytoskeleton in the Inner Life of the Cell video above?<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/research.ncsu.edu\/cmif\" target=\"_blank\" rel=\"noreferrer noopener\">NCSU \u2013 Cellular and Molecular Imaging Facilities(opens in new window)<\/a>&nbsp;&nbsp;&nbsp;(CMIF) \u2013&nbsp;<a href=\"https:\/\/research.ncsu.edu\/cmif\/galleries\/image-gallery\/\" target=\"_blank\" rel=\"noreferrer noopener\">Galleries(opens in new window)<\/a>&nbsp;&nbsp;&nbsp;\u2013 Click on the Galleries link to see a variety of images created from a variety of different types of microscopes, light, filters, stains and sample preparation.&nbsp;<\/p>\n\n\n\n<p class=\"has-black-color has-text-color has-link-color wp-elements-2 wp-block-paragraph\"><a href=\"https:\/\/www.aif.ncsu.edu\/\" target=\"_blank\" rel=\"noreferrer noopener\">NCSU \u2013 Analytical Instrumentation Facility (AIF)(opens in new window)<\/a>&nbsp;\u2013&nbsp;<a href=\"https:\/\/aif.ncsu.edu\/bio-em-images-3\/\" target=\"_blank\" rel=\"noreferrer noopener\">BIO EM Images<\/a>(opens in new window). This facility is located on Centennial Campus and <a href=\"https:\/\/aif.ncsu.edu\/biological-electron-microscopy-brochure\/\" target=\"_blank\" rel=\"noreferrer noopener\">offers&nbsp;short courses and information<\/a> on a variety in microscope imaging technologies.&nbsp;&nbsp;&nbsp;ck links<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/micro.magnet.fsu.edu\/\" target=\"_blank\" rel=\"noreferrer noopener\">Exploring the World of Optics and Microscopy(opens in new window)<\/a>&nbsp;&nbsp;\u2013 nice website featuring a wide variety of microscope technologies and images.<\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-3\">Course Modifications for Cells and Microscope Lab with Bacterial Projects: (not done Summer 2025)<\/h2>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\"><a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/BIO183-Gram-Staining-Act-Sp2015.pdf\" target=\"_blank\">Bacterial (Prokayotic Cells) Gram Staining Protocol Handout (not in your lab manual)<\/a> &#8211; incorporate into lab handouts and define new activities for student pairs.<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">For this modification, the Make Up Module does not include information on bacterial cells walls and gram staining&#8230;..<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-4\">Gram Staining:<\/h3>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"572\" height=\"453\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/Gram-Stain-reaction-test-Ekmonsaurus-2008.jpg\" alt=\"\" class=\"wp-image-762\" style=\"width:394px;height:312px\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/Gram-Stain-reaction-test-Ekmonsaurus-2008.jpg 572w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/Gram-Stain-reaction-test-Ekmonsaurus-2008-300x238.jpg 300w\" sizes=\"auto, (max-width: 572px) 100vw, 572px\" \/><\/figure>\n<\/div>\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"310\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-bacteria_med.jpg\" alt=\"\" class=\"wp-image-764\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-bacteria_med.jpg 720w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-bacteria_med-300x129.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color has-link-color wp-elements-5\"><strong>Bacterial outer cell wall structure:<\/strong><\/h3>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"805\" height=\"297\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-cell-walls_med.jpg\" alt=\"\" class=\"wp-image-760\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-cell-walls_med.jpg 805w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-cell-walls_med-300x111.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2019\/05\/gram-cell-walls_med-768x283.jpg 768w\" sizes=\"auto, (max-width: 805px) 100vw, 805px\" \/><\/figure>\n<\/div>\n\n\n<h3 class=\"wp-block-heading\">&nbsp;&nbsp;<\/h3>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Microscopes and Cells Scroll below for In Person lab information.&nbsp; For all In Person BIO 183 lab units &#8211; Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; Your lab handout will be collected and graded later. Lab Policies&nbsp; Lab [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":10,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-198","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/198","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/comments?post=198"}],"version-history":[{"count":76,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/198\/revisions"}],"predecessor-version":[{"id":5201,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/198\/revisions\/5201"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/media?parent=198"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}