{"id":2659,"date":"2021-10-19T17:18:56","date_gmt":"2021-10-19T21:18:56","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=2659"},"modified":"2026-10-11T20:16:33","modified_gmt":"2026-10-12T00:16:33","slug":"unit-4-lab-1-dna-fingerprinting-online-make-up","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-4-lab-1-dna-fingerprinting-online-make-up\/","title":{"rendered":"Unit 4: Lab 1 DNA Fingerprinting Online Make Up"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">&nbsp;<\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/Gel_electrophoresis_2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-65\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/05\/Gel_electrophoresis_2-150x150.jpg\" alt=\"DNA Gel\" width=\"150\" height=\"150\"><\/a><\/h2>\n\n\n\n<h2 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\"><strong>DNA Fingerprinting &#8211; Online Make Up Module<\/strong><\/span><\/h2>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later.<\/em><\/span><\/h4>\n\n\n\n<p class=\"has-vivid-red-color has-text-color wp-block-paragraph\"><span style=\"font-size: 12pt\"><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2024\/03\/DNA-FingerprintingHandoutB-for-2024.docx\" target=\"_blank\">Lab handout for DNA Fingerprinting <\/a>&#8211; Online Make Up Module<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Important Background Information to review:<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=jEJp7B6u_dY\" target=\"_blank\" rel=\"noreferrer noopener\">An Introduction to the Human Genome<\/a>&nbsp;\u2013 Basic Structure and Variation in Human DNA (5:32)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/restriction-video.html\" target=\"_blank\" rel=\"noreferrer noopener\">Restriction Enzymes and how they are used with DNA<\/a>&nbsp;&nbsp;\u2013 show how DNA with Restriction Enzymes\/Endonucleases and how strands can be put back together with DNA Ligase (another enzyme).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/dnalc.cshl.edu\/view\/15475-The-cycles-of-the-polymerase-chain-reaction-PCR-3D-animation.html\" target=\"_blank\">Polymerase Chain Reaction(opens in new window)<\/a>&nbsp;(PCR) 3D Animation Cold Springs Harbor&nbsp;&nbsp;\u2013 click on video \u2013 used to make larger samples of DNA from an initial small sample of DNA.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\">PCR Animation(opens in new window)<\/a>&nbsp;&nbsp;&nbsp;\u2013 a nice simple step by step animation to show how PCR works.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=7onjVBsQwQ8\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Fingerprinting using PCR<\/a>&nbsp;(opens in new window)to isolate and amplify Short Tandem Repeats (STR) in small DNA samples, then what they look like separated by DNA Electrophoresis. (4:09)<\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">DNA Gel with labeled known DNA fragment sizes (lane 1). This also shows the different banding pattern results (different sized fragments) produced when using different restriction enzymes on the same DNA.<\/span><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"400\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\" alt=\"DNA gel with different banding patterns\" class=\"wp-image-295\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">DNA Electrophoresis Equipment:<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a rel=\"noreferrer noopener\" href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/gelelectrophoresis-video.html\" target=\"_blank\">How DNA Electrophoresis works<\/a>&nbsp;\u2013 the electrophoretic process.<\/strong><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-8f761849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"308\" height=\"165\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg\" alt=\"DNA Gel boc and casting stand\" class=\"wp-image-300\" style=\"width:473px;height:253px\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg 308w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/lse_horizontal_apparatus_sub_main-300x161.jpg 300w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"600\" height=\"600\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg\" alt=\"Micropipette and tip\" class=\"wp-image-301\" style=\"width:288px;height:288px\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg 600w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-300x300.jpg 300w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1; Practice Pipetting &#8211; short videoclips<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=wrLFlxlduYc&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=2\" target=\"_blank\" rel=\"noopener noreferrer\">How to adjust the volume on your micropipetter<\/a><\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=uddfQmLx-yE&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=1\" target=\"_blank\" rel=\"noopener noreferrer\">How to use a micropipetter<\/a><\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 2: Restriction Digestion of DNA &#8211; view the background Information animations above.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3 &amp; 4: Preparation of the 0.8% Agarose Gel and Electrophoresis Unit and Preparing the DNA Samples and Loading the Gel<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\">In Activity Three, calculate and record the amount of agarose needed to make the indicated 0.8% agarose gels.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/html\/lse\/support\/tutorial-casting-an-agarose-gel-wndw.html\" target=\"_blank\" rel=\"noreferrer noopener\">Casting an Agarose Gel<\/a> &#8211; we will actually use a casting stand instead of tape to seal the gel tray.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=p-jwliaGFrk&amp;t=5s\" target=\"_blank\" rel=\"noreferrer noopener\">Loading an Agarose Gel<\/a> \u2013 tips for loading gels and what not to do.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=vq759wKCCUQ\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Electrophoresis Procedure<\/a>&nbsp; &#8211; loading and running an agarose gel (as you would have done in lab with the same equipment).<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=7ltGBsbWYgY\" target=\"_blank\" rel=\"noopener noreferrer\">Staining Agarose Gels with Fast Blast Blue<\/a> &#8211; In our teaching labs, we do not use Ethidium Bromide in our gels (it is a highly toxic mutagen and possible carcinogen) as indicated in the DNA Electrophoresis animations in the Background Information above.&nbsp; Instead we will use a non-toxic Fast Blast Blue stain that will bind to the DNA in the gels allowing us to visualize the bands of DNA.&nbsp;&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading has-pale-pink-color has-text-color\"><span style=\"color: #000000\">Activity 5: Analyzing the DNA Patterns<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">In our lab we will look at the PCR products from DNA left on the glass case compared to samples from 2 probable suspects and a negative DNA control sample.  <\/span>Why is it important to include a negative control on the gel?<\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Which of the samples\/suspects match the <\/span>DNA left on the glass case?<\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Sketch the stained DNA gel below and label the lanes.&nbsp; Answer the end of lab Discussion Questions #2-5 in your lab handout based upon this gel.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">We can look more closely at the size of sample DNA bands\/fragments by comparing the distance traveled in the gel to the known base pair size of the fragments found in the known DNA Standards\/Marker DNA.&nbsp;<\/span> This particular gel does not contain DNA Standards\/Marker DNA, but an example of Lambda HindIII DNA size Marker is included below.<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\">Stained BIORAD DNA Gel (click on image to enlarge)<\/td><td><span style=\"font-size: 12pt\">Stained BIORAD DNA Gel (lanes right to left)<\/span><\/td><td>Lambda <em>HindIII<\/em> DNA size Marker\/standard*<\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><img loading=\"lazy\" decoding=\"async\" width=\"219\" height=\"292\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2024\/03\/DNA-Gel-1-rotated.jpg\" alt=\"\"><\/td><td><span style=\"font-size: 12pt\">Lane 1 = <\/span>left empty\/blank<br><span style=\"font-size: 12pt\">Lane 2 = <\/span>left empty\/blank<br><span style=\"font-size: 12pt\">Lane 3 = <\/span>No DNA, control<br><span style=\"font-size: 12pt\">Lane 4 = <\/span>Early bidder<br><span style=\"font-size: 12pt\">Lane 5 = <\/span>Employee<br><span style=\"font-size: 12pt\">Lane 6 = <\/span>Evidence from glass   <br><span style=\"font-size: 12pt\">Lane 7 =<\/span> left empty\/blank<br><span style=\"font-size: 12pt\">Lane 8 = <\/span> left empty\/blank<\/td><td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/05\/Lamda-HindIII-marker.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2319\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/05\/Lamda-HindIII-marker-121x300.jpg\" alt=\"\" width=\"121\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/05\/Lamda-HindIII-marker-121x300.jpg 121w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2021\/05\/Lamda-HindIII-marker.jpg 130w\" sizes=\"auto, (max-width: 121px) 100vw, 121px\" \/><\/a><\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">,<\/figcaption><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">* <span style=\"font-size: 12pt\">depending on how long a gel is run, you may or may not see all the possible bands of DNA separate.<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\"><span style=\"color: #000000\"><a rel=\"noopener noreferrer\" href=\"https:\/\/learn.genetics.utah.edu\/content\/science\/forensics\/\" target=\"_blank\">How DNA Electrophoresis is used in forensics<\/a> &#8211;<\/span><span style=\"font-size: 14pt\"> Nice new webpage &#8211; topics include: Can DNA Demand a Verdict? How can DNA be used to identify an individual? Forensic DNA Analysis.&nbsp; Is DNA Analysis alone enough to acquit or convict? &nbsp;<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2020\/10\/Modified-crown-jewels-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Who Stole the Crown Jewels?<\/a> &#8211; a paper simulation will be performed in place of an actual in lab DNA electrophoresis. (<em>Carry out this simulations and add it to your lab handout<\/em>)&nbsp;<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li>From this handout you will need to \u201cadd\u201d the restriction enzyme to cut the long DNA sequences for each of the DNA samples on page 5.&nbsp; This Restriction enzyme will find sequences CCGG and cut between the C and G as shown in the example.<\/li>\n\n\n\n<li>Then&nbsp;<strong>count<\/strong>&nbsp;and<strong>&nbsp;label<\/strong>&nbsp;the # of base pairs (bp) in each of the new DNA fragments.<\/li>\n\n\n\n<li><strong>Sketch<\/strong>&nbsp;where these fragment will end up on the DNA gel diagram&nbsp;(page 4) comparing to the known bp units listed on the right of the paper gel.&nbsp;<em>Ignore the 12 bp fragment on the diagram. This is just to show location.<\/em><\/li>\n\n\n\n<li>Examine your completed paper gel and answer question #6 at the end of your DNA Lab Unit Handout.<\/li>\n\n\n\n<li>Be sure to include the completed pages 4, 5 &amp; 6 with your DNA Unit handouts. You will need to refer to these completed pages to support your answers to #6. You will not get credit for #6 if these completed pages are not provided and legible.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><strong><em>Be sure to insert this activity into your DNA Fingerprinting lab unit.<\/em><\/strong><\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><span style=\"color: #0000ff\">* If you do not have a printer<\/span>, you can write down the size for all the fragments for each of the subjects in your handout.&nbsp; Then sketch a labeled diagram of the resulting gel and explain your evidence for who stole the crown jewels.<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Basic DNA Structure<\/span><\/h3>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=t_7STCzrI_E\" target=\"_blank\" rel=\"noopener noreferrer\">The Human Genome Project<\/a>&nbsp;<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\" target=\"_blank\" rel=\"noreferrer noopener\">Molecular Visualization of DNA<\/a>&nbsp; &#8211; do not worry if you have not covered all of these topics in lecture yet.&nbsp; The animation will be helpful when you do review this material.<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">0-1:48 = DNA Structure &#8212; Also seen in the Mitosis and Meiosis lab (this part is important for lab this week)<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">The rest of this video with be taught later in lecture and be more applicable in the Transformation lab.<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">1:48-2:53 = DNA Replication &#8211;Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">2:53-4:48 = Transcription &#8211; (you will learn about this and Translation later in lecture)<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">4:48-6:57 = Translation &#8211;<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n\n\n\n<div style=\"height:26px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2024\/03\/BIO-183-Molecular-technique-descriptionB.docx\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Modern Molecular Techniques Assignment<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Click for details. This assignment will be due by Friday 11:59PM of the Off Lab week following the In Person DNA Fingerprinting Lab. &#8211; assignment will be linked here.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-black n_fill-black n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:19px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2024\/01\/Peer-Evaluation-Form-for-Off-Week-Group-Assign_Sp24.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Group Work Peer Evaluation form(opens in new window)<\/a>&nbsp;&nbsp;&nbsp;<\/strong>&nbsp;\u2013 each student should complete their own peer review and submit it on Moodle by the same due date as the Modern Molecular Techniques Assignment above. Late Peer Reviews will not be graded. You must indicate your own contributes on the peer review form to receive credit.<\/p>\n\n\n\n<div style=\"height:24px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div id=\"\" class=\"block-callout n_items-center-ns  n_bg-yellow   block-callout--img-left \" >\n\n\t\t\t\t\t\t<div class=\"block-callout__content n_pa4 n_w-100 n_flex n_flex-column n_tc  n_text-black\">\n\t\t\t\t                                    <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-1-final-projects\/\" class=\"block-callout__link n_no-underline \">\n\t\t\t            <P class=\"block-callout__heading heading n_overflow-wrap-break n_pv2 n_ph0 n_ttn n_ma0 n_lh-title n_f3  n_univers-light n_normal n_text-black\">Final Pathogen Projects due for next In Person lab week.<\/P>\n\n\t\t            <\/a>\n                \t\t\t\t<p class=\"block-callout__body-wrapper n_text-black n_ma0 n_pa0 \">\n\t\t\t\t\t<span class=\"block-callout__body \">Groups will present their Pathogen Project to the class. This will be recorded for self reflection. The final Pathogen paper is also due by the start of that In Person lab week. Click for details. Scroll down to the bottom of the Projects page.<\/span>\n\t\t\t\t\t                            \n    \n    \n        <span id=\"\" class=\"block-callout__arrow right-arrow icon n_text-black n_fill-black n_br0  n_s3 \" aria-hidden=\"true\">\n    <svg version=\"1.1\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"n_mw-100 n_w-100 n_h-100\" viewBox=\"0 0 32 32\"   ><path d=\"M16.438 9.563l-0.625 0.625 5.563 5.563h-13.375v0.875h13.375l-5.563 5.563 0.625 0.625 6.625-6.625z\"><\/path><\/svg><\/span>\n                    \t\t\t\t<\/p>\n\t\t\t<\/div>\n\t\t<\/div>\n\n\n\n<div style=\"height:31px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading has-black-color has-text-color\"><strong>Interesting Molecular Biology courses and opportunities at NCSU<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Opportunities for students:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/biotech.ncsu.edu\/minors-certificate\/\" target=\"_blank\" rel=\"noreferrer noopener\">Minor in Biotechnology at NCSU(opens in new window)<\/a>\u00a0\u00a0\u00a0(BIT Program) and\u00a0Courses in Biotech (<a href=\"https:\/\/biotech.ncsu.edu\/spring-2027-courses\/\">BIT\u00a0Spring 2027<\/a> &amp;\u00a0<a href=\"https:\/\/biotech.ncsu.edu\/fall-2026-courses\/\" target=\"_blank\" rel=\"noopener\">Fall 2026(opens in new window)<\/a><\/li>\n\n\n\n<li><a href=\"http:\/\/www.btec.ncsu.edu\/academic\/undergraduate\/undergraduate_minor.php\" target=\"_blank\" rel=\"noreferrer noopener\">Minor in Biomanufactoring at NCSU<\/a>&nbsp;(BTEC)<\/li>\n\n\n\n<li><a rel=\"noreferrer noopener\" href=\"https:\/\/units.cals.ncsu.edu\/master-of-microbial-biotechnology\/\" target=\"_blank\">NCSU Professional Sciences Master\u2019s Degree(opens in new window)<\/a>&nbsp;&nbsp;(MMB Program \u2013 Master\u2019s of Microbial Biotechnology)<\/li>\n\n\n\n<li><a href=\"http:\/\/www.btec.ncsu.edu\/about\/facilities.php\" target=\"_blank\" rel=\"noreferrer noopener\">Biomanufacturing on a larger scale at BTEC<\/a>&nbsp;(on Centennial Campus, click on Academic Programs to learn about available Undergraduate Programs )<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\"><strong>Important Technologies in Molecular Biology<\/strong><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/learn.genetics.utah.edu\/content\/science\/forensics\/\" target=\"_blank\" rel=\"noreferrer noopener\">How DNA Electrophoresis is used in forensics(opens in new window)<\/a>&nbsp;&nbsp;\u2013 Nice new webpage \u2013 topics include: Can DNA Demand a Verdict? How can DNA be used to identify an individual? Forensic DNA Analysis.&nbsp; Is DNA Analysis alone enough to acquit or convict? &nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2025\/03\/DNA-Profiling-Tracing-Killers-and-Solving-Mysteries-Using-Genetic-Clues.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Profiling: Tracing Killers and Solving Mysteries Using Genetic Clues<\/a> &#8211; nice article with overview of the development of DNA fingerprinting technologies linked to real cases.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-content\/uploads\/sites\/352\/2023\/06\/A-Not-So-Simple-Idea.pdf\" target=\"_blank\">Early ideas and Development of PCR(opens in new window)<\/a>&nbsp;&nbsp;\u2013 A Not So Simple Idea<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/learn.genetics.utah.edu\/content\/labs\/pcr\/\" target=\"_blank\">All About PCR(opens in new window)<\/a>&nbsp;&nbsp;\u2013 A good website with animations and interesting examples<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=2pp17E4E-O8\" target=\"_blank\" rel=\"noreferrer noopener\">Genome Editing with CRISPR-Cas9<\/a>&nbsp;a&nbsp;new and powerful technology with many applications.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.sciencenews.org\/article\/nobel-prize-chemistry-gene-editing-tool-crispr-wins?utm_source=Editors_Picks&amp;utm_medium=email&amp;utm_campaign=editorspicks100820\" target=\"_blank\" rel=\"noreferrer noopener\">Gene Editing Tool CRISPER Wins the Chemistry Nobel(opens in new window)<\/a>&nbsp;&nbsp;(2020)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\">ELISA Anitbody tests(opens in new window)<\/a>&nbsp;&nbsp;\u2013 animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/unit-4-labs-2-3-online\/\" target=\"_blank\">Gene Transformation(opens in new window)<\/a>&nbsp;&nbsp;\u2013 inserting new genes\/transformation -former BIO 183 lab unit<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"http:\/\/www.youtube.com\/watch?v=N7vxq948l-U\" target=\"_blank\" rel=\"noreferrer noopener\">Bioprocessing \u2013 Protein Production and Purification on an Industrial Scale<\/a><\/p>\n\n\n\n<h3 class=\"wp-block-heading has-black-color has-text-color\">Careers in Biotech \u2013&nbsp;<a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noreferrer noopener\">nice website.&nbsp;&nbsp;(opens in new window)<\/a>&nbsp;Careers&nbsp;at the Bench and Beyond<\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Short Reads:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"http:\/\/www.dnaforensics.com\/DNAFingerprinting.aspx\" target=\"_blank\" rel=\"noreferrer noopener\">The History of Genetic Fingerprinting<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a rel=\"noreferrer noopener\" href=\"https:\/\/news.ncsu.edu\/2021\/06\/irish-potato-famine-pathogen-stoked-outbreaks-on-six-continents\/\" target=\"_blank\">NCSU Researchers Irish Potato Famine Pathogen Stoked Outbreaks on 6 Continents(opens in new window)<\/a>&nbsp;&nbsp;\u2013 using a more specialized electrophoretic genotyping<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/news.ncsu.edu\/2011\/08\/mkgraves\/\" target=\"_blank\" rel=\"noreferrer noopener\">DNA \u201cFingerprint\u201d Can Aid Food Safety<\/a>&nbsp;\u2013 NCSU researcher<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; DNA Fingerprinting &#8211; Online Make Up Module Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later. Lab handout for DNA Fingerprinting &#8211; Online Make Up Module Important Background Information to review: An Introduction [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2659","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/2659","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/comments?post=2659"}],"version-history":[{"count":49,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/2659\/revisions"}],"predecessor-version":[{"id":5232,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/pages\/2659\/revisions\/5232"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-hybrid-lab\/wp-json\/wp\/v2\/media?parent=2659"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}