{"id":1281,"date":"2020-04-20T14:55:30","date_gmt":"2020-04-20T18:55:30","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1281"},"modified":"2023-02-01T20:10:51","modified_gmt":"2023-02-02T01:10:51","slug":"unit-2lab-1-online-lab","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-2lab-1-online-lab\/","title":{"rendered":"Unit 2:Lab 1 Microscopes and Cells \u2013 Online Make Up lab"},"content":{"rendered":"<h2><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/cheek_cells1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-63\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/cheek_cells1-150x150.jpg\" alt=\"Cheek Cells\" width=\"150\" height=\"150\" \/><\/a> <span style=\"color: #000000\">Microscopes and Cells &#8211;<\/span> <span style=\"color: #0000ff\">Online Make Up Lab<\/span><\/h2>\n<h4><span style=\"color: #000000\"><em>Read along in your lab handout with the information in this webpage.\u00a0 Fill in and answer all questions accordingly in your lab handout.\u00a0 Your lab handout will be collected and graded later.<\/em><\/span><\/h4>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/02\/Cells-Lab-HandoutF21-MakeUp2023.docx\" target=\"_blank\" rel=\"noopener\">Microscopes and Cells Lab Handout-Online <span style=\"color: #000000\">Make Up Module<\/span><\/a> &#8211; Includes areas for sketches and answering questions.\u00a0 You will need to make your sketches on paper and upload the fully labeled jpg images into the digital copy of this handout at the appropriate places OR use a computer\/tablet with drawing capability.<\/span><\/p>\n<h3><span style=\"color: #000000\">The Compound Light Microscope<\/span><\/h3>\n<p>\u00a0<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-187\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cm501microscopediagram.jpg\" alt=\"microscope with labeled parts\" width=\"500\" height=\"469\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cm501microscopediagram.jpg 500w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cm501microscopediagram-300x281.jpg 300w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<p>\u00a0<\/p>\n<h3 class=\"style11\" align=\"left\"><span style=\"color: #000000\">Helpful Links<\/span><\/h3>\n<p><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.biologycorner.com\/worksheets\/microscope_use.html\" target=\"_blank\" rel=\"noopener noreferrer\">Microscope Key Points, Diagrams, and Quizzes<\/a><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"http:\/\/micro.magnet.fsu.edu\/primer\/java\/scienceopticsu\/powersof10\/index.html\" target=\"_blank\" rel=\"noopener noreferrer\">Exploring Magnification: Power of Ten video<\/a><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=SUo2fHZaZCU\" target=\"_blank\" rel=\"noopener noreferrer\">How to use the microscope video<\/a><\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/AppendixA_micro08.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Appendix A<\/a> of your Lab Manual &#8211; Use and Care of Microscopes<\/span><\/p>\n<h3 class=\"style11\"><span style=\"color: #000000\">Reminders<\/span><\/h3>\n<ul class=\"style3\">\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\"><strong>Always start by using the 4X objective lens<\/strong>. Find the specimen and focus using the coarse focus, then the fine focus knob. <strong>Only use the coarse focus know with the 4X objective lens<\/strong>.\u00a0 Then progress to the 10X objective lens, then 40X lens and oil\/100X lenses. Be sure to focus at EACH <strong>new objective lens (10X, 40X, 100x) using ONLY the fine focus knob<\/strong>. Do not skip using a lens. Always progress one lens at a time.<\/span><\/li>\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\"><strong>Label all drawings<\/strong> with organisms name and indication of size: total magnification, field of view, size bar, or lens used in relation to the field of view.\u00a0 Total magnification = object lens x ocular lens.<\/span><\/li>\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">Other information: date, time, place and any relevant notes.<\/span><\/li>\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">Record all observations; don&#8217;t rely on memory.<\/span><\/li>\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">Make sure to record what you SEE, not what you THINK you should see.<\/span><\/li>\n<li class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">Detail is key, pay attention to even the smallest things.<\/span><\/li>\n<\/ul>\n<h3>\u00a0<\/h3>\n<h3 class=\"style11\"><span style=\"color: #000000\">A<\/span><span style=\"color: #000000\">ctivity 1:\u00a0 Procedure 1A <\/span><span style=\"color: #000000\">\u00a0<\/span><\/h3>\n<h3><span style=\"color: #000000\">Simulation Activity to help you learn how to use a Microscope &#8211;<\/span>-directions in lab manual\/handout<\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">We will use the<a href=\"http:\/\/www.ncbionetwork.org\/iet\/microscope\/\" target=\"_blank\" rel=\"noopener noreferrer\"> Virtual Microscope<\/a> developed by the NC Community Colleges BioNetwork.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">You will need to click on the &#8220;LEARN&#8221; button found at the bottom of the page.\u00a0 If you do not see 5 buttons at the bottom of your computer screen, you may need to elongate the open window.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Work your way through all of the prompts indicted by &#8220;?&#8221; to review the names of all the major parts of the microscope and a few associated supplies needed for microscopy(microscope oil, lens paper and Kimwipes).\u00a0 This light microscope has a 10X ocular lens and 4 different objective lenses (4X, 10X, 40X and 100x\/oil lens).\u00a0 This very closely matches the microscopes used in both BIO 181\/183 labs and other life science courses.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Click the &#8220;EXPLORE&#8221; button and click on the &#8220;?&#8221; to open the box of prepared slides.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Select ONE of the &#8220;Bacteria slides&#8221;.\u00a0 Use the course focus to find the specimen with the 4X lens.\u00a0 First use the Course Focus, then the Fine Focus adjustors to see the best image of the specimen.\u00a0 You may need to adjust the level of light intensity as well.\u00a0 Continue to increase magnification until you can best see the cells.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Draw a few of the cells in the indicated circles in your lab manual.\u00a0 Keep in mind to draw the cell size in relation to the field of view.\u00a0 Indicate the name of the slide, total magnification you thought was most appropriate, and any structures you can discern.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Repeat by selecting one of the Human (tissues) slides, and also one of the Plant (tissues) slides, and draw and label\u00a0 accordingly.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Answer the questions found in the lab handout\/Lab Unit write up.<\/span><\/li>\n<\/ul>\n<h3>\u00a0<\/h3>\n<h3><span style=\"color: #000000\">Activity 1: Procedure 1B<\/span><\/h3>\n<h3><span style=\"color: #000000\">Practice <em>Interpretation of Provided Microscope Images<\/em>\u00a0<\/span><\/h3>\n<h3 class=\"style11\"><span style=\"color: #000000\">In lab you would have practiced using a microscope to see the following sorts of images.\u00a0 \u00a0Choose one of these prepared slide images below &#8211; draw what you see, indicate the total magnification, and label at least 2 structures you can see within your organism.<\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">Below are images of bacteria, animal, plant and fungal cells. Besides the Key to the Images, what visual cues can you see that would help you determine if they are bacterial, animal, plant or fungal cells?\u00a0 Think back to BIO 181 labs or characteristics of cells belonging in different Kingdoms.\u00a0 What information is useful?\u00a0 What else would you need to know when looking under a light microscope?\u00a0 You can search the internet to help with labeling 2 structures within the image you choose to draw,<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Compare the cells of the image you selected in Procedure 1B to ONE of the slides you viewed in the Virtual Microscope above.\u00a0 Compare how they are alike, and how they are different.<\/span><\/li>\n<\/ul>\n<p class=\"style10\"><span style=\"font-size: 14pt\"><strong>1.<\/strong><\/span><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-189\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell1-150x150.jpg\" alt=\"Cell 1\" width=\"200\" height=\"200\" \/>\u00a0 \u00a0 \u00a0<span style=\"font-size: 14pt\"><strong>2.<\/strong><\/span> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-190\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell2-300x300.jpg\" alt=\"Cell 2\" width=\"200\" height=\"200\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell2-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell2-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell2.jpg 600w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/>\u00a0 \u00a0 \u00a0\u00a0<span style=\"font-size: 14pt\"><strong>3.<\/strong><\/span> <img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-191\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell4-e1528914451404-300x300.jpg\" alt=\"Cell 4\" width=\"200\" height=\"200\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell4-e1528914451404-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell4-e1528914451404-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Cell4-e1528914451404.jpg 360w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/><\/p>\n<p class=\"style10\"><span style=\"font-size: 14pt\"><strong>4.<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-192\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/bact1-e1528914586726-300x300.jpg\" alt=\"bacteria 1\" width=\"200\" height=\"200\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/bact1-e1528914586726-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/bact1-e1528914586726-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/bact1-e1528914586726.jpg 324w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/>\u00a0 \u00a0 \u00a05.<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-193\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669-300x300.jpg\" alt=\"Cells 3b\" width=\"200\" height=\"200\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669-768x768.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669-1024x1024.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/cell3B-e1528914769669.jpg 1535w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/>\u00a0 \u00a0 \u00a06.<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-194\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fungus-e1528914852251-300x300.jpg\" alt=\"fungus\" width=\"200\" height=\"200\" \/><\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt\">Key to images:<\/span><\/p>\n<ol>\n<li><span style=\"font-size: 12pt\">Plant epidermis whole mount, 40X objective lens &amp; 10X ocular lens.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Nerve cell, 40X objective lens &amp; 10X ocular lens.\u00a0 Stained cells.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Whole mount plant epidermis cells, 40X objective lens &amp; 10X ocular lens.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Bacterial cells, 100X objective lens &amp; 10X ocular lens.\u00a0 Stained cells.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Muscle cells, 40X objective lens &amp; 10X ocular lens.\u00a0 Stained cells.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Fungal cells, 40X objective lens &amp; 10X ocular lens.\u00a0 Stained cells.<\/span><\/li>\n<\/ol>\n<h3><span style=\"color: #000000\">Activity 1:\u00a0 Procedure 1C <\/span><span style=\"color: #000000\">Simple Staining with Animal and Plant Cells (Eukaryotic Cells)<\/span><\/h3>\n<p class=\"style10\"><span style=\"font-size: 12pt\">Simple staining is the addition of ONE type to stain that can allow you to better see cells and\/or structures under the light microscope.<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">In lab you would have made your own slides cheek and onions cells and stained with toluidine blue.\u00a0 Using the light microscopes, you would see the following images at low, medium, and high power (4X, 10X, and 40X objective lenses), using your compound microscope.<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">Examine the images of check and onion cells slides below.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Decide which magnification is most appropriate and draw both in the appropriate location in your lab handout.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Be sure to label the structures that you can see in each of the pictures.\u00a0 At a minimum, you should be able to label 2 structures found in these cells.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Based in these slides, how are these cells similar?\u00a0 How are they different?<\/span><\/li>\n<\/ul>\n<p><span style=\"color: #000000;font-size: 12pt\"><strong>Unstained cheek cell slides at low, medium and high powers.<\/strong><\/span><\/p>\n<p>a. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1297\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-129.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a> b. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1296 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-130.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>c.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-131-e1587417817241.jpeg\">\u00a0\u00a0<\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1770 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-131.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt\"><strong>Stained cheek cell slides at low, medium and high power.\u00a0 What is the benefit of staining these cells?<\/strong><\/span><\/p>\n<p>a. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1773 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-126.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>\u00a0 b. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127.jpeg\">.<\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1305 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-127.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>\u00a0 c. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1304 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/Image-128.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt\">Stained onion cell slides at low, medium and high power.<\/span><\/p>\n<p>a. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1775 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-132.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>. b. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1776 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-133.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a> c.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1777 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-134.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">How would you describe the animal cells? The plant cells? Size, shape organization? What\u00a0<strong>structures and organelles<\/strong> can you see that help you identify the type\/kingdom of these cells? What cellular organelles can&#8217;t you see? Why can&#8217;t you see them?\u00a0 What is the best size to view these animal and plant cells?<\/span><\/p>\n<p>\u00a0<\/p>\n<h3><span style=\"color: #000000\">Activity 2: Procedure 2A Osmosis in Plant Cells (<em>Elodea<\/em>)<\/span><\/h3>\n<p><span style=\"font-size: 12pt\"><strong><em>Elodea<\/em> is a freshwater aquatic plant.\u00a0 <\/strong><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">The following images show an <em>Elodea<\/em> leaf mounted using freshwater at low 40X, medium100X, and high power 400X total magnification.\u00a0 <\/span><\/li>\n<li><span style=\"font-size: 12pt\">Steps 1 was followed.\u00a0 <\/span><\/li>\n<li><span style=\"font-size: 12pt\">Draw the <em>Elodea<\/em> cells at the appropriate magnification in the leftmost circle in your lab handout.<\/span><\/li>\n<\/ul>\n<p>a. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1779 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-113.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>. b. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1780 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-115.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>\u00a0 c. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1781 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-116.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>\u00a0<\/p>\n<ul>\n<li><span style=\"font-size: 12pt\"><em>In the images below (d &amp; e), the Elodea<\/em> leaf was flushed with 5% saline (d), and then 10% saline (e) following steps #2 in your lab handout.\u00a0\u00a0<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Draw the 5% and 10% cells in the middle (for 5%) and rightmost circle (for the 10%) in your lab handout.\u00a0<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Write a note in your handout that nothing changed when freshwater was flushed back through the cells on 10% saline.\u00a0 The 5% slides was able to &#8220;recover&#8221;.\u00a0\u00a0Why might that be, but not with the 10% treatment?\u00a0 The two images below are viewed at high power.\u00a0\u00a0<\/span><\/li>\n<\/ul>\n<p>d.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1784 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-119.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>. e. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120.jpeg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1783 size-medium\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120-300x225.jpeg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120-300x225.jpeg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120-768x576.jpeg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120-1024x768.jpeg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/08\/Image-120.jpeg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt\"><strong>You may find this videos showing osmosis in Red Onion Cells helpful in understanding how changing environmental conditions\u00a0 on plant cells works in relation to osmosis.<\/strong><\/span><\/p>\n<p class=\"style10\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-209\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/redOnion.jpg\" alt=\"Red onion cells\" width=\"260\" height=\"185\" \/><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\"><a class=\"style11\" href=\"https:\/\/www.youtube.com\/watch?v=Mp_CJBqRI5A\" target=\"_blank\" rel=\"noopener noreferrer\">Video: Osmosis in Red Onion Epidermis<\/a> &#8211; (1:41) flushing various concentrations of saline solution through these onion cell walls and flushing back with freshwater after a 5% solution.<\/span><\/p>\n<h3>\u00a0<\/h3>\n<h3 class=\"has-pale-pink-color has-text-color\">Activity 2: Procedure 2B Osmosis in Blood Cells<\/h3>\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3556\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/02\/SEM-of-Blood-cells.jpg\" alt=\"\" width=\"602\" height=\"461\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/02\/SEM-of-Blood-cells.jpg 602w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/02\/SEM-of-Blood-cells-300x230.jpg 300w\" sizes=\"auto, (max-width: 602px) 100vw, 602px\" \/>\n<figcaption>Scanning Electron Micrograph showing 3 types of blood cells.<\/figcaption>\n<\/figure>\n<p>\u00a0<\/p>\n<ul>\n<li>View the Video: Osmosis in Red Blood Cells found below.<\/li>\n<li>Draw, label and explain what you see under all 3 conditions (distilled water, 0.9% saline, 10% saline (salt water) show in the video.\u00a0 Use the appropriate osmotic terminology (isotonic, hypotonic and hypertonic) in your explanations of what happens to the red blood cells in each of these conditions.<\/li>\n<li>Also be sure to answer any other written questions.<\/li>\n<\/ul>\n<p class=\"style10\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-211 alignnone\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/red_blood_cells-300x225.jpg\" alt=\"red blood cells\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/red_blood_cells-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/red_blood_cells-768x576.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/red_blood_cells-1024x768.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\"><a class=\"style11\" href=\"https:\/\/www.youtube.com\/watch?v=A8cI6FkcG4c\" target=\"_blank\" rel=\"noopener noreferrer\">Video: Osmosis in Red Blood Cells<\/a>-(3:47) see how red blood cells appear in isotonic, hypertonic and hypotonic solutions.<\/span><\/p>\n<p><span style=\"font-size: 12pt\"><strong>Reviewing BOTH of the above videos should help you better understand the osmosis plant and animal cells.\u00a0 cells.<\/strong><\/span><\/p>\n<p>\u00a0<\/p>\n<h3><span style=\"color: #000000\">Activity 3: Procedure 2C Cell Membranes: Osmosis and Differential Permeability<\/span><\/h3>\n<p><span style=\"font-size: 12pt\">Be sure to read the Introduction in your lab handout.\u00a0 The 2 pictures below (a and b) show the set up following steps 1-6.\u00a0 Write a hypothesis, your predictions and explain why at step 7 in your lab handout.<\/span><\/p>\n<p><span style=\"font-size: 12pt\">Record the initial colors of the tube contents and solution in the beaker in step #8.<\/span><\/p>\n<p>a.<a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0282-e1587957201492.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1404\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0282-e1587957201492-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0282-e1587957201492-225x300.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0282-e1587957201492.jpg 480w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a>. b.\u00a0\u00a0<a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0284-e1587957075688.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1405\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0284-e1587957075688-225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0284-e1587957075688-225x300.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0284-e1587957075688.jpg 480w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt\">The following pictures (below) show the color changes after running the experiment.\u00a0 The first and second pictures (a and b) show the color of the solution in the beaker (yellow) and in the dialysis tube (black) at the end of the experiment.\u00a0 Compare the color of the starch solution inside the dialysis tube and also the color of the solution in the beaker before and after the experiment.\u00a0\u00a0\u00a0The picture on the right (c) shows the beaker after the dialysis tube is removed and 1 ml of starch was added to the yellow solution left in the beaker (step#12).<\/span><\/p>\n<p><span style=\"font-size: 12pt\">Fill in and answer steps #8-16 in your lab<\/span><\/p>\n<p>c.\u00a0 <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0304-e1587957354260.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1409\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0304-e1587957354260-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0304-e1587957354260-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0304-e1587957354260.jpg 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>\u00a0 d. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0306-e1587957495762.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1408\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0306-e1587957495762-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0306-e1587957495762-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0306-e1587957495762.jpg 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a>. e. <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0310-e1587957619172.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1407\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0310-e1587957619172-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0310-e1587957619172-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/04\/IMG_0310-e1587957619172.jpg 640w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p>\u00a0<\/p>\n<p><span style=\"font-size: 12pt\">Use these provided student data to fill in Table 1<\/span><\/p>\n<p>\u00a0<\/p>\n<p><strong><span style=\"font-size: 12pt\"> Table 1.\u00a0 Provided raw data from 1 student group.\u00a0<\/span><\/strong><\/p>\n<table style=\"height: 89px;width: 53.7393%;border-collapse: collapse;border-style: ridge\">\n<tbody>\n<tr>\n<td style=\"width: 16.6729%;text-align: center;border-style: solid\">\u00a0<\/td>\n<td style=\"width: 25.0239%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\"><strong>Time<\/strong><\/span><\/td>\n<td style=\"width: 23.5624%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\"><strong>Mass (grams)<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 16.6729%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\"><strong>Initial (I)<\/strong><\/span><\/td>\n<td style=\"width: 25.0239%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\">12:10 PM<\/span><\/td>\n<td style=\"width: 23.5624%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\">17.49<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 16.6729%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\"><strong>Final (F)<\/strong><\/span><\/td>\n<td style=\"width: 25.0239%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\">2:45 PM<\/span><\/td>\n<td style=\"width: 23.5624%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\">18.95<\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 16.6729%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\"><strong>Change (F-I)<\/strong><\/span><\/td>\n<td style=\"width: 25.0239%;text-align: center;border-style: solid\"><span style=\"font-size: 12pt\">_______ minutes<\/span><\/td>\n<td style=\"width: 23.5624%;text-align: center;border-style: solid\">\u00a0<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong><span style=\"font-size: 12pt\">Table 2.\u00a0 Osmosis Data from 4 Pairs of Students<\/span><\/strong><\/p>\n<table style=\"height: 138px;width: 95.4708%;border-collapse: collapse;border-style: solid\">\n<tbody>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\"><strong>Pair data<\/strong><\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\"><strong>Change in Time (minutes)<\/strong><\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\"><strong>Change in Mass (+\/- grams)<\/strong><\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">Data provided in Table 1<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">\u00a0<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">\u00a0<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">Group 1<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">55<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+0.39<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">Group 2<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">55<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+0.45<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">Group 3<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">110<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+0.66<\/td>\n<\/tr>\n<tr style=\"height: 23px\">\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">Group 4<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">114<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+0.53<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 33.3333%;border-style: solid;text-align: center\">Group 5<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">115<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+0.89<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 33.3333%;border-style: solid;text-align: center\">Group 6<\/td>\n<td style=\"width: 33.3333%;height: 23px;border-style: solid;text-align: center\">112<\/td>\n<td style=\"width: 28.8041%;height: 23px;border-style: solid;text-align: center\">+1.49<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 12pt\">Why might we see differences?\u00a0 Look back to the procedure and pictures above &#8230;..<\/span><\/p>\n<p><strong><span style=\"color: #0000ff\">Scroll down to the yellow Osmosis Cells Assignment box at the bottom of this webpage to access the assignment.<\/span><\/strong><\/p>\n<h3><span style=\"color: #000000\">Activity 3: Animated Film &#8211; The Inner Life of the Cell<\/span><\/h3>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-212\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/InLifCell.jpg\" alt=\"Inner Life of the Cell video still\" width=\"240\" height=\"187\" \/><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\"><a class=\"style11\" title=\"Inner Life of Cell video\" href=\"https:\/\/www.youtube.com\/watch?v=wJyUtbn0O5Y\" target=\"_blank\" rel=\"noopener noreferrer\">Video: Cellular Visions: Inner Life of the Cell video<\/a>\u00a0(3:12)- Read the summary below before you watch the video. Click on the link and scroll down and click the play video arrow.<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\"><strong>Video Background:<\/strong>\u00a0 This video starts with a view of the inside of a blood vessel.\u00a0 You will see the red blood cells rushing past and the much larger white blood cells rolling along the wall of the blood vessel.\u00a0 The white blood cell receives an extracellular signal and the majority of the video shows what happens inside the white blood cell as it responds to that signal.\u00a0 The final scene shows the white blood cell reshaping (due to the conformational changes of the cytoskeleton) and slipping through the endothelial cells of the blood vessel wall to the site of inflammation.<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">As you watch the video, how many structures\/organelles can you see? Can you find -protein filaments, lipid rafts, plasma membrane, actin filaments, microtubules, motor proteins, transport vesicles, mitochondria, centrosomes, nucleus, nuclear pore, mRNA, ribosomes making proteins, ER, golgi apparatus, vesicles transported back to the plasma membrane surface.<\/span><\/p>\n<p>\u00a0<\/p>\n<h3>Additional Information:<\/h3>\n<p><span style=\"font-size: 14pt\"><a class=\"style11\" href=\"http:\/\/news.ncsu.edu\/2013\/08\/tp-adler-cancer\/\" target=\"_blank\" rel=\"noopener noreferrer\">Article: NCSU Researcher \u201cStopping Cancer in its Track\u201d<\/a> \u2013 Examines the role to which the cytoskeleton\/actin is involved in movement of cancer cells.\u00a0 Remember &#8220;moving through&#8221; the cytoskeleton in the Inner Life of the Cell video above?<\/span><\/p>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/research.ncsu.edu\/cmif\" target=\"_blank\" rel=\"noopener noreferrer\">NCSU \u2013 Cellular and Molecular Imaging Facilities<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>\u00a0<i class=\"fas fa-external-link-alt fa-sm\" aria-hidden=\"true\"><\/i>\u00a0(CMIF) \u2013\u00a0<a href=\"https:\/\/research.ncsu.edu\/cmif\/galleries\/image-gallery\/\" target=\"_blank\" rel=\"noopener noreferrer\">Galleries<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>\u00a0<i class=\"fas fa-external-link-alt fa-sm\" aria-hidden=\"true\"><\/i>\u00a0\u2013 Click on the Galleries link to see a variety of images created from a variety of different types of microscopes, light, filters, stains and sample preparation.\u00a0 \u00a0(Located in Gardner Hall)<\/span><\/p>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.aif.ncsu.edu\/\" target=\"_blank\" rel=\"noopener noreferrer\">NCSU \u2013 Analytical Instrumentation Facility (AIF)<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>\u00a0<i class=\"fas fa-external-link-alt fa-sm\" aria-hidden=\"true\"><\/i>\u00a0\u2013\u00a0<a href=\"https:\/\/www.aif.ncsu.edu\/bio-em-images\/\" target=\"_blank\" rel=\"noopener noreferrer\">BIO EM Images<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>\u00a0<i class=\"fas fa-external-link-alt fa-sm\" aria-hidden=\"true\"><\/i>. This facility is located on Centennial Campus and offers\u00a0<a href=\"https:\/\/www.aif.ncsu.edu\/education\/\" target=\"_blank\" rel=\"noopener noreferrer\">short courses and information<span class=\"screen-reader-text\">(opens in new window)<\/span><\/a>\u00a0<i class=\"fas fa-external-link-alt fa-sm\" aria-hidden=\"true\"><\/i>\u00a0on a variety in microscope imaging technologies.\u00a0\u00a0\u00a0<\/span><\/p>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/micro.magnet.fsu.edu\/\" target=\"_blank\" rel=\"noopener noreferrer\">Exploring the World of Optics and Microscopy<\/a> &#8211; nice website featuring a wide variety of microscope technologies and images.<\/span><\/p>\n\n","protected":false},"excerpt":{"rendered":"<p>Microscopes and Cells &#8211; Online Make Up Lab Read along in your lab handout with the information in this webpage.\u00a0 Fill in and answer all questions accordingly in your lab handout.\u00a0 Your lab handout will be collected and graded later. Microscopes and Cells Lab Handout-Online Make Up Module &#8211; Includes areas for sketches and answering [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":12,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1281","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1281","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=1281"}],"version-history":[{"count":140,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1281\/revisions"}],"predecessor-version":[{"id":3591,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1281\/revisions\/3591"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=1281"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}