{"id":1545,"date":"2020-05-08T13:36:50","date_gmt":"2020-05-08T17:36:50","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1545"},"modified":"2023-06-14T09:40:20","modified_gmt":"2023-06-14T13:40:20","slug":"unit-4-lab-1-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-lab-1-online\/","title":{"rendered":"Unit 4: Lab 1 DNA Fingerprinting &#8211; In Person"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">&nbsp;<\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/Gel_electrophoresis_2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-65\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/Gel_electrophoresis_2-150x150.jpg\" alt=\"DNA Gel\" width=\"150\" height=\"150\"><\/a><\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><span style=\"color: #000000\">DNA Fingerprinting &#8211; In Person<\/span><\/h2>\n\n\n\n<p><span style=\"font-size: 14pt\"><span style=\"color: #000000\">Scroll below for In Person Lab unit<\/span><\/span><\/p>\n\n\n\n<p><\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-lab-1-dna-fingerprinting-online-make-up\/\" style=\"border-radius:8px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later.<\/em><\/span><\/h4>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/03\/2_Modified-DNA-FingerprintingHandout-for-Spring23.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Lab handout for DNA Fingerprinting<\/a> &#8211; In-Person<\/span><span style=\"color:#000000\" class=\"tadv-color\"> for Spring 2023<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Important Background Information to review:<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=jEJp7B6u_dY\">An Introduction to the Human Genome<\/a><\/span> <span style=\"font-size: 12pt\">&#8211; Basic Structure and Variation in Human DNA (5:32)<\/span><\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/pcr.html\" target=\"_blank\">Polymerase Chain Reaction (PCR)(opens in new window)<\/a>&nbsp;&nbsp;\u2013 click on video &#8211; used to make larger samples of DNA from an initial small sample of DNA.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noreferrer noopener\">PCR Animation(opens in new window)<\/a>&nbsp;&nbsp;\u2013 a nice simple step by step animation to show how PCR works.<\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a rel=\"noopener noreferrer\" href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/restriction-video.html\" target=\"_blank\">Restriction Enzymes and how they are used with DNA<\/a>&nbsp; and&nbsp; <a rel=\"noopener noreferrer\" href=\"https:\/\/www.youtube.com\/watch?v=piGOuud3f40\" target=\"_blank\">Restriction Enzyme animation<\/a><\/span><\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/www.google.com\/search?q=pcr+and+dna+fingerprinting&amp;tbm=vid&amp;sa=X&amp;ved=2ahUKEwjx4o3fmPH9AhVBOUQIHVUVCbQQ0pQJegQIChAB&amp;biw=1359&amp;bih=704&amp;dpr=2#fpstate=ive&amp;vld=cid:a534510e,vid:7onjVBsQwQ8\" target=\"_blank\">DNA Fingerprinting using PCR <\/a>to isolate and amplify Short Tandem Repeats (STR) in small DNA samples. (4:09)<\/p>\n\n\n\n<p><a rel=\"noopener noreferrer\" href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/gelelectrophoresis-video.html\" target=\"_blank\"><span style=\"font-size: 12pt\">How DNA Electrophoresis works<\/span><\/a> &#8211; more in depth on the electrophoretic process.<\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">DNA Gel with labeled known DNA fragment sizes\/DNA Ladder\/Marker DNA (lane 1). This also shows the different banding pattern results (different sized fragments) produced when using different restriction enzymes on the same DNA.<\/span><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"400\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\" alt=\"DNA gel with different banding patterns\" class=\"wp-image-295\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">DNA Electrophoresis Equipment:<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"308\" height=\"165\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg\" alt=\"DNA Gel boc and casting stand\" class=\"wp-image-300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg 308w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main-300x161.jpg 300w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-300x300.jpg\" alt=\"Micropipette and tip\" class=\"wp-image-301\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg 600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1; Practice Pipetting &#8211; short videoclips<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=wrLFlxlduYc&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=2\" target=\"_blank\" rel=\"noopener noreferrer\">How to adjust the volume on your micropipetter<\/a><\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=uddfQmLx-yE&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=1\" target=\"_blank\" rel=\"noopener noreferrer\">How to use a micropipetter<\/a><\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 2: Restriction Digestion of DNA &#8211; view the background Information animations above.<\/span>  <span style=\"color:#1500a3\" class=\"tadv-color\">The DNA Kit used this semester provides DNA that is already pre-digested, but it is still important to understand how restriction enzymes work and their importance in DNA electrophoresis.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3 &amp; 4: Preparation of the 0.8% Agarose Gel and Electrophoresis Unit and Preparing the DNA Samples and Loading the Gel<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\">In Activity Three, calculate and record the amount of agarose needed to make the indicated 0.8% agarose gels.<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/html\/lse\/support\/tutorial-casting-an-agarose-gel-wndw.html\">Casting an Agarose Gel<\/a> &#8211; we will actually use a casting stand instead of tape to seal the gel tray.<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=vq759wKCCUQ\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Electrophoresis Procedure<\/a>&nbsp; &#8211; loading and running an agarose gel (as you will do in lab with the same equipment).<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=7ltGBsbWYgY\" target=\"_blank\" rel=\"noopener noreferrer\">Staining Agarose Gels with Fast Blast Blue<\/a> &#8211; In our teaching labs, we will use a non-toxic Fast Blast Blue stain that will bind to the DNA in the gels allowing us to visualize the bands of DNA.&nbsp;&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 5: Analyzing the DNA Patterns edit<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">In our lab we will look at the pattern and location of the Crime Scene DNA on the gel and look to see which of the suspects match the same pattern for all the bands.<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Sketch and label the lanes\/samples in your stained DNA gel.&nbsp; Include this sketch in your lab handout.&nbsp; Answer all the end of lab Discussion Questions #1-5 in your lab handout based upon your stained gel.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">We can look more closely at the size of sample DNA bands\/fragments by comparing the distance traveled in the gel to the known base pair size of the fragments found in the known DNA Standards\/Marker DNA.&nbsp; How many bands of the DNA Marker can you see in your own gel?&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-regular\"><table><tbody><tr><td><strong>Lambda <em>HindIII<\/em> DNA size Marker\/standard*<\/strong><br><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/05\/Lamda-HindIII-marker.jpg\"><\/a><\/td><\/tr><tr><td><img loading=\"lazy\" decoding=\"async\" width=\"130\" height=\"322\" class=\"wp-image-2197\" style=\"width: 130px\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/Lamda-HindIII-marker.jpg\" alt=\"\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/Lamda-HindIII-marker.jpg 130w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/Lamda-HindIII-marker-121x300.jpg 121w\" sizes=\"auto, (max-width: 130px) 100vw, 130px\" \/><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">* <span style=\"font-size: 12pt\">depending on how long a gel is run, you may or may not see all the bands separate.<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><a href=\"https:\/\/learn.genetics.utah.edu\/content\/science\/forensics\/\" target=\"_blank\" rel=\"noopener noreferrer\">How DNA Electrophoresis is used in forensics<\/a> &#8211;<\/span><span style=\"font-size: 14pt\"> Nice new webpage &#8211; topics include: Can DNA Demand a Verdict? How can DNA be used to identify an individual? Forensic DNA Analysis.&nbsp; Is DNA Analysis alone enough to acquit or convict? &nbsp;<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity to be run during the down time of lab:<\/span> &nbsp;&nbsp;<span style=\"color: #000000\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Modified-crown-jewels-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Who Stole the Crown Jewels?<\/a> &#8211; This is a paper simulation that clearly demonstrates how restriction enzymes work and how DNA fragments separate out during DNA electrophoresis. (<em>Carry out this simulations and add&nbsp; completed pages 4-6 to your DNA lab handout<\/em>)&nbsp;<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\">To reduce printing&nbsp; &#8211; you should read this activity online.&nbsp; We will provide each student with a paper copy of the gel (page 4) and the un-cut DNA sequences (page 5) and the Final Report (page 6).<span style=\"color: #ff0000\">*&nbsp;<\/span><\/span><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #ff0000;font-size: 12pt\"><span style=\"color: #000000\">From this handout you will need to &#8220;add&#8221; the restriction enzyme to cut the long DNA sequences for each of the DNA samples.&nbsp; This Restriction enzyme will find sequences CCGG and cut between the C and G as shown in the example.<\/span><\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Then count and label the # of base pair (bp) in each of the new DNA fragments.<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Sketch where these fragment will end up on the DNA gel diagram.<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Examine your completed paper gel and complete page 6 in the Crown Jewels handout.&nbsp; Who Stole the Crown Jewels?&nbsp; How do you know this?<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt;color: #0000ff\">Be sure to include the completed pages 4, 5 and 6 with your DNA Unit handouts.<\/span><\/li>\n<\/ul>\n\n\n\n<p><span style=\"color: #ff0000\">*<\/span> <span style=\"font-size: 10pt\">If you find you need a paper copy of the whole activity, we can print a few copies per lab section.&nbsp; Here you will need to <strong>cut<\/strong> the provided paper DNA Samples with a <strong>restriction enzyme<\/strong> at the set sequences of base pair units using actual scissors.&nbsp; Then you will tape these DNA fragments\/pieces on a paper gel diagram according to the base pair size of each fragment.&nbsp;&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Basic DNA Structure<\/span><\/h3>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=t_7STCzrI_E\" target=\"_blank\" rel=\"noopener noreferrer\">The Human Genome Project<\/a>&nbsp;<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\">Molecular Visualization of DNA<\/a>&nbsp; &#8211; do not worry if you have not covered all of these topics in lecture yet.&nbsp; The animation will be helpful when you do review this material.<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">0-1:48 = DNA Structure &#8212; Also seen in the Mitosis and Meiosis lab (this part is important for lab this week)<\/span><\/p>\n\n\n\n<p><span style=\"color: #000000;font-size: 12pt\">The rest of this video with be taught later in lecture and be more applicable in the Transformation lab.<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">1:48-2:53 = DNA Replication &#8211;Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">2:53-4:48 = Transcription &#8211; (you will learn about this and Translation later in lecture)<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">4:48-6:57 = Translation &#8211;<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n\n\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/03\/BIO-183-DNA-Pres-ExpectationsSelf-Assess-Sp2023.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation Expectations and Self Assessment<\/a> <span style=\"color: #000000\">&#8211; 30 points total<\/span><\/h3>\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/03\/DNAPresEvalSheetTA_2023docx.docx\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation TA Evaluation Sheet<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/10\/Peer-Evaluation-Form-for-DNA-Presentations.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Peer Evaluation form for the DNA Presentation<\/a><\/h3>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><strong>Important Technologies in Molecular Biology<\/strong><\/span><\/h3>\n\n\n\n<p><a href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/pcr.html\" target=\"_blank\" rel=\"noopener noreferrer\">Polymerase Chain Reaction (PCR)<\/a> &#8211; used to make larger samples of DNA from an initial small amount of sample.<\/p>\n\n\n\n<p><a rel=\"noopener noreferrer\" href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\">PCR Animation<\/a>\u00a0\u2013 a nice simple step by step animation to show how PCR works.<\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/06\/A-Not-So-Simple-Idea.pdf\" target=\"_blank\">Early ideas and Development of PCR<\/a> &#8211; A Not So Simple Idea<\/p>\n\n\n\n<p><a href=\"https:\/\/www.youtube.com\/watch?v=2pp17E4E-O8\">Genome Editing with CRISPR-Cas9<\/a> a&nbsp;new and powerful technology with many applications.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.sciencenews.org\/article\/nobel-prize-chemistry-gene-editing-tool-crispr-wins?utm_source=Editors_Picks&amp;utm_medium=email&amp;utm_campaign=editorspicks100820\" target=\"_blank\" rel=\"noopener noreferrer\">Gene Editing Tool CRISPER Wins the Chemistry Nobel<\/a> (2020)<\/p>\n\n\n\n<p><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a>&nbsp;\u2013 animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Careers in Biotech \u2013<\/span>&nbsp;<a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noopener noreferrer\">nice website.&nbsp;&nbsp;<\/a><span style=\"color: #000000\">Careers&nbsp;at the Bench and Beyond<\/span><\/h3>\n\n\n\n<p>Short Reads:<\/p>\n\n\n\n<p><a href=\"http:\/\/www.dnaforensics.com\/DNAFingerprinting.aspx\">The History of Genetic Fingerprinting<\/a><\/p>\n\n\n\n<p><a href=\"http:\/\/genetics.thetech.org\/original_news\/news16\">DNA Fingerprinting is Everywhere<\/a>&nbsp;-discusses some applications.<\/p>\n\n\n\n<p><a rel=\"noreferrer noopener\" href=\"https:\/\/news.ncsu.edu\/2021\/06\/irish-potato-famine-pathogen-stoked-outbreaks-on-six-continents\/\" target=\"_blank\">NCSU Researchers Irish Potato Famine Pathogen Stoked Outbreaks on 6 Continents<\/a> &#8211; using a more specialized electrophoretic genotyping<\/p>\n\n\n\n<p><a href=\"https:\/\/news.ncsu.edu\/2011\/08\/mkgraves\/\">DNA &#8220;Fingerprint&#8221; Can Aid Food Safety<\/a>&nbsp;&#8211; NCSU researcher<\/p>\n\n\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; DNA Fingerprinting &#8211; In Person Scroll below for In Person Lab unit Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later. Lab handout for DNA Fingerprinting &#8211; In-Person for Spring 2023 Important Background [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":20,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1545","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1545","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=1545"}],"version-history":[{"count":136,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1545\/revisions"}],"predecessor-version":[{"id":3777,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1545\/revisions\/3777"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=1545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}