{"id":1548,"date":"2020-05-08T13:44:39","date_gmt":"2020-05-08T17:44:39","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=1548"},"modified":"2023-05-10T22:14:20","modified_gmt":"2023-05-11T02:14:20","slug":"unit-4-labs-2-3-online","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-labs-2-3-online\/","title":{"rendered":"Unit 4: Labs 2 &amp; 3 Gene Transformation \u2013 In Person"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/GFP-jellyfish.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"150\" height=\"150\" class=\"alignnone size-thumbnail wp-image-66\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/GFP-jellyfish-150x150.jpg\" alt=\"Glowing jellyfish\"><\/a><\/h2>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Gene Transformation &#8211; In Person<\/h2>\n\n\n\n<p>Scroll below for In Person Lab unit<\/p>\n\n\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link has-vivid-red-color has-cyan-bluish-gray-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-labs-2-3-gene-transformation-make-up-module\/\" style=\"border-radius:8px\" target=\"_blank\" rel=\"noreferrer noopener\">Click for Online Make Up Module<\/a><\/div>\n<\/div>\n\n\n\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/11\/Gene-TransformationWk1_Fall21_InPersonOnline.docx\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Lab Handout for Gene Transformation lab &#8211; Part 1<\/span><\/a><\/p>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Read along in your Part 1 and Part 2 (see below) lab handouts with the information on this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; These will be collected and graded later.<\/em><\/span><\/h4>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Parts 1 and 2 will cover 2 weeks of lab.<\/em><\/span><\/h4>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"225\" height=\"224\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg\" alt=\"pGlo Plasmid DNA\" class=\"wp-image-312\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-150x150.jpg 150w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"457\" height=\"154\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg\" alt=\"pGlo plasmid and transformed bacterial culture\" class=\"wp-image-313\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg 457w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle-300x101.jpg 300w\" sizes=\"auto, (max-width: 457px) 100vw, 457px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"style11 wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Good background information.&nbsp; Also read the Additional Background Information in your lab handout.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.youtube.com\/watch?v=Ik_Pxht1LM0\">Using Restriction Enzymes and DNA Ligase to make plasmids<\/a>&nbsp;<span style=\"color: #000000\">(1:38)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"https:\/\/www.youtube.com\/watch?v=_HdMkSEQU8k\">Stanford Scientists Produce Cancer Drugs from Rare Plants<\/a>&nbsp;<span style=\"color: #000000\">(1:59) <span style=\"font-size: 14pt\">by making plasmids and inserting them into more easily grown plants or yeasts.<\/span><\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"http:\/\/www.youtube.com\/watch?v=GmhD-RWNL6c&amp;sns=fb\">Funding Basic Science to Revolutionize Medicine<\/a>&nbsp;<span style=\"color: #000000\">(3:59) <span style=\"font-size: 14pt\">that show how the focus in research has changed over the past 50 years.<\/span><\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a class=\"style11\" href=\"http:\/\/www.youtube.com\/watch?v=Sl2PRHGpYuU&amp;feature=related\">How GFP is used in research<\/a><span class=\"style11\">&nbsp;<span style=\"color: #000000\">(10:03)<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/www.youtube.com\/watch?v=c40UudFIlGw&amp;t=2s\" target=\"_blank\" rel=\"noopener noreferrer\">Video of BIORAD pGlo Transformation procedure<\/a><span style=\"color: #000000\"> (7:01) <span style=\"font-size: 14pt\">&#8211; what we will do in lab.&nbsp;<\/span><\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"141\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome-300x141.jpg\" alt=\"\" class=\"wp-image-1949\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome-300x141.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome.jpg 320w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1: In-Lab discussion with your TA &#8211; Important Concepts in the Transformation Procedures:<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGlo-Plasmid.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">pGlo Plasmid<\/a> <span style=\"color: #000000;font-size: 14pt\">we will use in lab.&nbsp; Why are these genes important?&nbsp; What do they code for?<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span class=\"style11\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/transformation2.html\">DNA transformation using plasmids<\/a>&nbsp;<span style=\"color: #000000;font-size: 14pt\">&#8211; animation explaining the steps in our Transformation procedure.&nbsp; It is very important to understand the nature of the bacterial cell wall, why CaCl<sub>2<\/sub> is used, and why the heat shock is important.<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Answer the Protocol Discussion questions #1-8.<\/span><\/h3>\n\n\n\n<p><strong>Measurement marks on disposable plastic pipettes provided in the BIORAD kit:<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"720\" height=\"540\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg\" alt=\"pipet measurements\" class=\"wp-image-314\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg 720w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram-300x225.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3 &#8211; Experimental Treatments and Proposed Results<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #000000;font-size: 12pt\">Fill out the table at the end of this handout to indicate what you think the results would be for each of the 4 agar plates that were inoculated in this procedure.&nbsp; <strong>Also<\/strong> fill out the gray boxes for the agar plates that were not inoculated, but still indicate what you&#8217;d expect to happen with those nutrient conditions for the bacterial growth plates that did and did not get the plasmid added.&nbsp; Include why you think you&#8217;d get these results in ALL the boxes as well.<\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">End of Transformation Part 1 (week 1)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"has-pale-pink-color has-text-color wp-block-heading\"><span style=\"color: #000000\">Start of Transformation Part 2 (week 2).<\/span><\/h3>\n\n\n\n<h2 class=\"wp-block-heading\"><span style=\"color: #000000\">Transformation Efficiency Calculation<\/span><\/h2>\n\n\n\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/10\/Transformation-Wk2-Efficiency-Calculations-InPerson.docx\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Lab Handout for Gene Transformation Calculations &#8211; Part 2 In Person lab<\/span><\/a><span style=\"font-size: 12pt;color: #000000\"> &#8211; read before coming to lab.&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1: Data Collection from Transformation Plates<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Your Results:&nbsp; Record your bacterial growth on the different nutrient agar plates under white light and UV light.<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">Record your data in Table 1 in your lab handout that corresponds to your assigned Group.&nbsp; Be sure to collect data under both White Light and the UV Light.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Answer questions #2-6.&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000;font-family: UniversCondensed, Arial, sans-serif;font-size: 21px;font-weight: bold\">Activity 2: Calculating Transformation Efficiency<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">Read through the provided information and follow the prompts to calculate the transformation efficiency on your assigned <strong>+LB\/amp\/ara agar plate.<\/strong>&nbsp; <em>Be sure<\/em> to include the <em>units<\/em> as you set up each of the equations.&nbsp; &nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Transformation Efficiency data from each lab group will be recorded and shared on the class whiteboard.&nbsp; <\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Answer all the Discussion Questions at the end of the lab unit.&nbsp;&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=plVk4NVIUh8&amp;t=14s\" target=\"_blank\" rel=\"noopener noreferrer\">The Evolution of Bacteria on a Mega-Plate Petri Dish<\/a> &#8211; Natural mutation for antibiotic resistance in bacteria growing on nutrient agar containing an antibiotic.<\/span><\/p>\n\n\n\n<h3 class=\"style10 wp-block-heading\"><a href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\">Molecular Visualization of DNA&nbsp;<\/a><\/h3>\n\n\n\n<p class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">0-1:48 = DNA Structure &#8212; Also seen in the Mitosis and Meiosis lab<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">1:48-2:53 = DNA Replication &#8211;Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">2:53-4:48 = Transcription &#8211; (you will learn about this and Translation later in lecture)<\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"font-size: 12pt\"><span style=\"color: #000000\">4:48-6:57 = Translation &#8211; <\/span><span style=\"color: #000000\">(you will learn about this and Translation later in lecture)<\/span><\/span><\/p>\n\n\n\n<p class=\"style10\"><span style=\"color: #000000;font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n\n\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/03\/BIO-183-DNA-Pres-ExpectationsSelf-Assess-Sp2023.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation Expectations and Self Assessment<\/a> <span style=\"color: #000000\">&#8211; 30 points total<\/span><\/h3>\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2023\/03\/DNAPresEvalSheetTA_2023docx.docx\" target=\"_blank\" rel=\"noreferrer noopener\">DNA Presentation TA Evaluation Sheet<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/10\/Peer-Evaluation-Form-for-DNA-Presentations.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Peer Evaluation form for DNA Group Presentations<\/a><\/h3>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p class=\"has-medium-font-size\"><strong>Additional Information on Biotechnology<\/strong>:<\/p>\n\n\n\n<h3 class=\"style11 wp-block-heading\"><span style=\"color: #000000\">Check out the opportunities for undergraduate students! Certificate, Minor, Courses&#8230;..(look under Academic Programs)<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Link to information on a Minor in the<\/span> <a rel=\"noopener noreferrer\" href=\"https:\/\/biotech.ncsu.edu\/minors-certificate\/\" target=\"_blank\">Biotechnology Program at NCSU<\/a>&nbsp;<span style=\"color: #000000\">and<\/span>&nbsp;<a href=\"https:\/\/biotech.ncsu.edu\/spring-2023-2\/\" target=\"_blank\" rel=\"noreferrer noopener\">Courses in Biotechnology (BIT) at NCSU<\/a><\/h3>\n\n\n\n<h3 class=\"style11 wp-block-heading\"><span style=\"color: #000000\">Link to the<\/span> <a href=\"https:\/\/www.btec.ncsu.edu\/academic\/undergraduate\/index.php\" target=\"_blank\" rel=\"noopener noreferrer\">Undergraduate Programs at the BTEC center on Centennial Campus NCSU<\/a>&nbsp;<span style=\"color: #000000\">&#8211; where industry and education meet.<\/span><\/h3>\n\n\n\n<h3 class=\"style11 wp-block-heading\"><span style=\"color: #000000;font-size: 14pt\">At BTEC they use similar transformation procedures to engineer bacteria and yeasts to make (biomanufacturing) chemicals, medicines, vaccines, enzymes, proteins, biofuels&#8230;&#8230;.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Careers in Biotech &#8211; <a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noopener noreferrer\">nice website.&nbsp; <\/a>Careers&nbsp;at the Bench and Beyond<\/span><\/h3>\n\n\n\n<h3 class=\"style11 wp-block-heading\"><span style=\"color: #000000\">Great Short articles related to increasingly important Biotechnology&nbsp;<\/span><\/h3>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.conncoll.edu\/ccacad\/zimmer\/GFP-ww\/cooluses0.html\" target=\"_blank\" rel=\"noopener noreferrer\">GFP &#8211; Green Fluorescent Protein<\/a> &#8211; Cool Uses<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.genetics.org\/content\/199\/2\/293\" target=\"_blank\" rel=\"noopener noreferrer\">Brianbow<\/a> &#8211; New Resources and Emerging Biological Applications for Multicolor Genetic Labeling and Analysis &#8211; Informative and beautiful images!<\/span><\/p>\n\n\n\n<p><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">PCR Animation<\/span><\/a><span style=\"font-size: 12pt\"> &#8211; a nice simple step by step animation to show how PCR works.<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a> &#8211; animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/span><\/p>\n\n\n\n<p><span style=\"font-size: 12pt\"><a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/11\/Biology_of_SARS-CoV-2-1.pdf\" target=\"_blank\">Biology of SARS-CoV-2<\/a> &#8211; nice powerpoint with basic explanations<\/span><\/p>\n\n\n\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/07\/Ten-Years-of-CRISPR.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Ten Years of CRISPR<\/a> &#8211; highlights 7 ways that CRISPR has been used in gene editing<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Gene Transformation &#8211; In Person Scroll below for In Person Lab unit Lab Handout for Gene Transformation lab &#8211; Part 1 Read along in your Part 1 and Part 2 (see below) lab handouts with the information on this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; These will be collected [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":16,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1548","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1548","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=1548"}],"version-history":[{"count":111,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1548\/revisions"}],"predecessor-version":[{"id":3773,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/1548\/revisions\/3773"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=1548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}