{"id":221,"date":"2018-06-13T15:01:56","date_gmt":"2018-06-13T19:01:56","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=221"},"modified":"2021-04-04T18:46:17","modified_gmt":"2021-04-04T22:46:17","slug":"protein-purification-and-chromatography","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/protein-purification-and-chromatography\/","title":{"rendered":"Protein Purification and Chromatography"},"content":{"rendered":"<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-380\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-1.jpg\" alt=\"GFP Structure\" width=\"200\" height=\"200\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-1.jpg 160w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-1-150x150.jpg 150w\" sizes=\"auto, (max-width: 200px) 100vw, 200px\" \/>\u00a0<\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/aequorea_gfp.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-381\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/aequorea_gfp-300x199.jpg\" alt=\"Jellyfish and GFP\" width=\"300\" height=\"199\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/aequorea_gfp-300x199.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/aequorea_gfp-768x510.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/aequorea_gfp-1024x680.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-1.jpg\">\u00a0<\/a><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-382\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-2.jpg\" alt=\"Protein separation column\" width=\"131\" height=\"200\" \/><\/a><\/p>\n<p class=\"style14\">Transformed bacteria grown in LB\/amp\/ara broth under UV light.<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/glowingflasks.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-384 \" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/glowingflasks-1024x576.jpg\" alt=\"Transformed bacteria under UV\" width=\"469\" height=\"264\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/glowingflasks-1024x576.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/glowingflasks-300x169.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/glowingflasks-768x432.jpg 768w\" sizes=\"auto, (max-width: 469px) 100vw, 469px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/www.youtube.com\/watch?v=Sl2PRHGpYuU&amp;feature=related\">A Green Light for Biology &#8211; Making the Invisible Visible<\/a>\u00a0(video) (10:03)<\/p>\n<p><a href=\"http:\/\/www.conncoll.edu\/ccacad\/zimmer\/GFP-ww\/GFP-Nobel.html\">GFP Green Fluorescent Protein<\/a>\u00a0&#8211; check out some of these cools links &#8211; Nobel Prize, uses, techniques, history,&#8230;..<\/p>\n<p><a href=\"http:\/\/www.youtube.com\/watch?v=giYQAdExKdE\">GFP Purification Procedure<\/a>\u00a0(video, an extension of the Transformation lab) (3:34).<\/p>\n<p><a href=\"http:\/\/www.youtube.com\/watch?v=VkdU2RryFOM\">More detailed GFP Purification Procedure- narrated<\/a>\u00a0(6:37)<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-386\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic-1024x591.jpg\" alt=\"Diagram of protein separation\" width=\"430\" height=\"248\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic-1024x591.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic-300x173.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic-768x443.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/gfppic.jpg 1147w\" sizes=\"auto, (max-width: 430px) 100vw, 430px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/GFP_chromatography.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-387\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/GFP_chromatography-300x226.jpg\" alt=\"Protein column and rinses\" width=\"467\" height=\"352\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/GFP_chromatography-300x226.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/GFP_chromatography.jpg 350w\" sizes=\"auto, (max-width: 467px) 100vw, 467px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Flo-proteins.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-389\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Flo-proteins-300x118.jpg\" alt=\"Various fluorescent proteins\" width=\"376\" height=\"148\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Flo-proteins-300x118.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Flo-proteins.jpg 357w\" sizes=\"auto, (max-width: 376px) 100vw, 376px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<h3>Opportunities at NCSU<\/h3>\n<ul class=\"style2\">\n<li class=\"style10\"><a href=\"https:\/\/biotech.ncsu.edu\/minors-certificate\/\" target=\"_blank\" rel=\"noopener noreferrer\">Minor in Biotechnology at NCSU<\/a> (BIT Program) and <a href=\"https:\/\/biotech.ncsu.edu\/spring-2021\/\" target=\"_blank\" rel=\"noopener noreferrer\">Courses in Biotech (BIT)<\/a><\/li>\n<\/ul>\n<ul class=\"style2\">\n<li class=\"style10\"><a href=\"http:\/\/www.btec.ncsu.edu\/academic\/undergraduate\/undergraduate_minor.php\">Minor in Biomanufactoring at NCSU<\/a>\u00a0(BTEC)<\/li>\n<\/ul>\n<ul class=\"style2\">\n<li class=\"style10\"><a href=\"http:\/\/harvest.cals.ncsu.edu\/master-of-microbial-biotechnology\/\">NCSU Professional Sciences Master&#8217;s Degree<\/a>\u00a0(MMB Program &#8211; Master&#8217;s of Microbial Biotechnology)<\/li>\n<\/ul>\n<ul class=\"style2\">\n<li class=\"style10\"><a href=\"http:\/\/www.btec.ncsu.edu\/about\/facilities.php\">Biomanufacturing on a larger scale at BTEC<\/a>\u00a0(on Centennial Campus)<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/www.youtube.com\/watch?v=N7vxq948l-U\">Bioprocessing &#8211; Protein Production and Purification on an Industrial Scale<\/a>\u00a0(large volumes!) (19:52) There is a small segment in the middle (about 4:44) that discusses the hydrophillic\/hydrophobic nature of the column beads, proteins and salt washes.\u00a0 Other parts also discuss separation by charge and size using different types of columns.<\/p>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-390\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant-1024x768.jpg\" alt=\"Large fermentation tanks\" width=\"640\" height=\"480\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant-1024x768.jpg 1024w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant-300x225.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant-768x576.jpg 768w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/fermentation-plant.jpg 1772w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u00a0 Transformed bacteria grown in LB\/amp\/ara broth under UV light. &nbsp; A Green Light for Biology &#8211; Making the Invisible Visible\u00a0(video) (10:03) GFP Green Fluorescent Protein\u00a0&#8211; check out some of these cools links &#8211; Nobel Prize, uses, techniques, history,&#8230;.. GFP Purification Procedure\u00a0(video, an extension of the Transformation lab) (3:34). More detailed GFP Purification Procedure- narrated\u00a0(6:37) [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"parent":0,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-221","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/221","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=221"}],"version-history":[{"count":10,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/221\/revisions"}],"predecessor-version":[{"id":2245,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/221\/revisions\/2245"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=221"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}