{"id":2659,"date":"2021-10-19T17:18:56","date_gmt":"2021-10-19T21:18:56","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=2659"},"modified":"2023-05-10T22:12:48","modified_gmt":"2023-05-11T02:12:48","slug":"unit-4-lab-1-dna-fingerprinting-online-make-up","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-lab-1-dna-fingerprinting-online-make-up\/","title":{"rendered":"Unit 4: Lab 1 DNA Fingerprinting Online Make Up"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">&nbsp;<\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/Gel_electrophoresis_2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-65\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/Gel_electrophoresis_2-150x150.jpg\" alt=\"DNA Gel\" width=\"150\" height=\"150\"><\/a><\/h2>\n\n\n\n<h2 class=\"wp-block-heading\"><span style=\"color: #000000\">DNA Fingerprinting &#8211; Online Make Up Module<\/span><\/h2>\n\n\n\n<h4 class=\"wp-block-heading\"><span style=\"color: #000000\"><em>Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later.<\/em><\/span><\/h4>\n\n\n\n<p class=\"has-vivid-red-color has-text-color wp-block-paragraph\"><a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/10\/DNA-FingerpritingHandout_OnlineModule.docx\" target=\"_blank\"><span style=\"font-size: 12pt\">Lab handout for DNA Fingerprinting<\/span><\/a><span style=\"font-size: 12pt\"> &#8211; Online Make Up Module<\/span> &#8211; this is based on a different DNA kit than the In-Person lab handout.  Students making up this lab should use this handout and website to complete the lab.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Important Background Information to review:<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=jEJp7B6u_dY\">An Introduction to the Human Genome<\/a><\/span> <span style=\"font-size: 12pt\">&#8211; Basic Structure and Variation in Human DNA<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/restriction.html\">Restriction Enzymes and how they are used with DNA&nbsp;<\/a> and&nbsp; <a href=\"https:\/\/www.youtube.com\/watch?v=piGOuud3f40\" target=\"_blank\" rel=\"noopener noreferrer\">Restriction Enzyme animation<\/a><\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/gelelectrophoresis.html\">How DNA Electrophoresis works<\/a><\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">DNA Gel with labeled known DNA fragment sizes (lane 1). This also shows the different banding pattern results (different sized fragments) produced when using different restriction enzymes on the same DNA.<\/span><\/p>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"400\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/tumblr_m4ns61kC7u1qkssp4o1_500.gif\" alt=\"DNA gel with different banding patterns\" class=\"wp-image-295\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">DNA Electrophoresis Equipment:<\/span><\/h3>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"308\" height=\"165\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg\" alt=\"DNA Gel boc and casting stand\" class=\"wp-image-300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main.jpg 308w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/lse_horizontal_apparatus_sub_main-300x161.jpg 300w\" sizes=\"auto, (max-width: 308px) 100vw, 308px\" \/><\/a><\/figure>\n\n\n\n<figure class=\"wp-block-image\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-300x300.jpg\" alt=\"Micropipette and tip\" class=\"wp-image-301\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-300x300.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul-150x150.jpg 150w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/Wholesale-Single-Channel-Fixed-Volume-Pipette-Precision-50ul-manual-Micropipette-laboratory-industry-CHPipetteG50ul.jpg 600w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 1; Practice Pipetting &#8211; short videoclips<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=wrLFlxlduYc&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=2\" target=\"_blank\" rel=\"noopener noreferrer\">How to adjust the volume on your micropipetter<\/a><\/span><\/li>\n\n\n\n<li><span style=\"color: #000000;font-size: 12pt\"><a style=\"color: #000000\" href=\"https:\/\/www.youtube.com\/watch?v=uddfQmLx-yE&amp;list=PL0i_Vk36L8_zfFmZmY01PRdKLxrb6YMOb&amp;index=1\" target=\"_blank\" rel=\"noopener noreferrer\">How to use a micropipetter<\/a><\/span><\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 2: Restriction Digestion of DNA &#8211; view the background Information animations above.<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 3 &amp; 4: Preparation of the 0.8% Agarose Gel and Electrophoresis Unit and Preparing the DNA Samples and Loading the Gel<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\">In Activity Three, calculate and record the amount of agarose needed to make the indicated 0.8% agarose gels.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/html\/lse\/support\/tutorial-casting-an-agarose-gel-wndw.html\">Casting an Agarose Gel<\/a> &#8211; we will actually use a casting stand instead of tape to seal the gel tray.<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=vq759wKCCUQ\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Electrophoresis Procedure<\/a>&nbsp; &#8211; loading and running an agarose gel (as you would have done in lab with the same equipment).<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=7ltGBsbWYgY\" target=\"_blank\" rel=\"noopener noreferrer\">Staining Agarose Gels with Fast Blast Blue<\/a> &#8211; In our teaching labs, we do not use Ethidium Bromide in our gels (it is a highly toxic mutagen and possible carcinogen) as indicated in the DNA Electrophoresis animations in the Background Information above.&nbsp; Instead we will use a non-toxic Fast Blast Blue stain that will bind to the DNA in the gels allowing us to visualize the bands of DNA.&nbsp;&nbsp;<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Activity 5: Analyzing the DNA Patterns<\/span><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><span style=\"font-size: 12pt\">In our lab we will look at the pattern and location of the Crime Scene DNA on the gel and look to see which of the suspects match the same pattern for all the bands.<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">Sketch the stained BIORAD DNA gel below and label the lanes.&nbsp; Answer the end of lab Discussion Questions #2-5 in your lab handout based upon this gel.&nbsp;<\/span><\/li>\n\n\n\n<li><span style=\"font-size: 12pt\">We can look more closely at the size of sample DNA bands\/fragments by comparing the distance traveled in the gel to the known base pair size of the fragments found in the known DNA Standards\/Marker DNA.&nbsp;<\/span><\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Stained BIORAD DNA Gel (click on image to enlarge)<\/td><td><span style=\"font-size: 12pt\">Stained BIORAD DNA Gel (lanes right to left)<\/span><\/td><td>Lambda <em>HindIII<\/em> DNA size Marker\/standard*<\/td><\/tr><tr><td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/183-DNA-Gels-.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-2192 alignleft\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/183-DNA-Gels--225x300.jpg\" alt=\"\" width=\"225\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/183-DNA-Gels--225x300.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/03\/183-DNA-Gels--768x1024.jpg 768w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/td><td><span style=\"font-size: 12pt\">Lane 1 = Crime Scene<\/span><br><span style=\"font-size: 12pt\">Lane 2 = Suspect 1<\/span><br><span style=\"font-size: 12pt\">Lane 3 = Suspect 2<\/span><br><span style=\"font-size: 12pt\">Lane 4 = Suspect 3<\/span><br><span style=\"font-size: 12pt\">Lane 5 = Suspect 4<\/span><br><span style=\"font-size: 12pt\">Lane 6 = Suspect 5<\/span><br><span style=\"font-size: 12pt\">Lane 7 = Lambda <em>HindIII<\/em>\/DNA size   marker\/standard<\/span><br><span style=\"font-size: 12pt\">Lane 8 = Blank (no added DNA)<\/span><\/td><td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/05\/Lamda-HindIII-marker.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-2319\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/05\/Lamda-HindIII-marker-121x300.jpg\" alt=\"\" width=\"121\" height=\"300\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/05\/Lamda-HindIII-marker-121x300.jpg 121w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/05\/Lamda-HindIII-marker.jpg 130w\" sizes=\"auto, (max-width: 121px) 100vw, 121px\" \/><\/a><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><tr><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">* <span style=\"font-size: 12pt\">depending on how long a gel is run, you may or may not see all the bands separate.<\/span><\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><a href=\"https:\/\/learn.genetics.utah.edu\/content\/science\/forensics\/\" target=\"_blank\" rel=\"noopener noreferrer\">How DNA Electrophoresis is used in forensics<\/a> &#8211;<\/span><span style=\"font-size: 14pt\"> Nice new webpage &#8211; topics include: Can DNA Demand a Verdict? How can DNA be used to identify an individual? Forensic DNA Analysis.&nbsp; Is DNA Analysis alone enough to acquit or convict? &nbsp;<\/span><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\">ONLINE Lab Activity &#8211; if you missed lab this week:<\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Modified-crown-jewels-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">Who Stole the Crown Jewels?<\/a> &#8211; a paper simulation will be performed in place of an actual in lab DNA electrophoresis. (<em>Carry out this simulations and add it to your lab handout<\/em>)&nbsp;<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\">Since you were<span style=\"color: #000000\"> NOT able to perform this in a lab with equipment, we are providing a paper simulation for to complete at home.&nbsp; <a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Modified-crown-jewels-2.pdf\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"color: #0000ff\">Print*<\/span><\/a> out the activity and follow the<\/span> directions.&nbsp; Here you will to <strong>cut<\/strong> the provided DNA Samples with a <strong>restriction enzyme<\/strong> at the set sequences of base pair units using actual scissors.&nbsp; Then you will place these DNA fragments\/pieces on a paper gel diagram according to the base pair size of each fragment.&nbsp; Be sure to examine the provided example of the procedure before proceeding with the actual police report and the DNA sequences from the crime scene and the 4 suspects.&nbsp; Who Stole the Crown Jewels?<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\">Insert this activity into your DNA Fingerprinting lab unit.&nbsp; <span style=\"color: #0000ff\">Include a picture of your completed labeled paper gel or a labeled sketch <strong>including base pair unit #s<\/strong> and completed pages 4-6 of the Crown Jewel activity with your DNA Fingerprinting Lab Handout.<\/span><\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"font-size: 12pt\"><span style=\"color: #0000ff\">* If you do not have a printer<\/span>, you can write down the size for all the fragments for each of the subjects in your handout.&nbsp; Then sketch a labeled diagram of the resulting gel and explain your evidence for who stole the crown jewels (page 6).<\/span><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Basic DNA Structure<\/span><\/h3>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=t_7STCzrI_E\" target=\"_blank\" rel=\"noopener noreferrer\">The Human Genome Project<\/a>&nbsp;<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\"><a href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\">Molecular Visualization of DNA<\/a>&nbsp; &#8211; do not worry if you have not covered all of these topics in lecture yet.&nbsp; The animation will be helpful when you do review this material.<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">0-1:48 = DNA Structure &#8212; Also seen in the Mitosis and Meiosis lab (this part is important for lab this week)<\/span><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><span style=\"color: #000000;font-size: 12pt\">The rest of this video with be taught later in lecture and be more applicable in the Transformation lab.<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">1:48-2:53 = DNA Replication &#8211;Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">2:53-4:48 = Transcription &#8211; (you will learn about this and Translation later in lecture)<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">4:48-6:57 = Translation &#8211;<\/span><\/p>\n\n\n\n<p class=\"style10 wp-block-paragraph\"><span style=\"font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n\n\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/03\/BIO-183-DNA-Pres-ExpectationsSelf-Assess-Sp2022.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Presentation Expectations and Self Assessment<\/a> <span style=\"color: #000000\">&#8211; 30 points total<\/span><\/h3>\n\n\n\n<h3 class=\"style9 wp-block-heading\"><a rel=\"noopener noreferrer\" href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/03\/DNAPresEvalSheetTA_2022.docx\" target=\"_blank\">DNA Presentation Evaluation Sheet<\/a><\/h3>\n\n\n\n<h3 class=\"wp-block-heading\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/10\/Peer-Evaluation-Form-for-DNA-Presentations.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Peer Evaluation form for the DNA Presentation<\/a><\/h3>\n\n\n\n<div style=\"height:33px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\"><strong>Important Technologies in Molecular Biology<\/strong><\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/dnalc.cshl.edu\/resources\/animations\/pcr.html\" target=\"_blank\" rel=\"noopener noreferrer\">Polymerase Chain Reaction (PCR)<\/a> &#8211; used to make larger samples of DNA from an initial small amount of sample.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Animation<\/a>&nbsp;\u2013 a nice simple step by step animation to show how PCR works.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.youtube.com\/watch?v=2pp17E4E-O8\">Genome Editing with CRISPR-Cas9<\/a> a&nbsp;new and powerful technology with many applications.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.sciencenews.org\/article\/nobel-prize-chemistry-gene-editing-tool-crispr-wins?utm_source=Editors_Picks&amp;utm_medium=email&amp;utm_campaign=editorspicks100820\" target=\"_blank\" rel=\"noopener noreferrer\">Gene Editing Tool CRISPER Wins the Chemistry Nobel<\/a> (2020)<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a>&nbsp;\u2013 animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\"><span style=\"color: #000000\">Careers in Biotech \u2013<\/span>&nbsp;<a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noopener noreferrer\">nice website.&nbsp;&nbsp;<\/a><span style=\"color: #000000\">Careers&nbsp;at the Bench and Beyond<\/span><\/h3>\n\n\n\n<p class=\"wp-block-paragraph\">Short Reads:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"http:\/\/www.dnaforensics.com\/DNAFingerprinting.aspx\">The History of Genetic Fingerprinting<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"http:\/\/genetics.thetech.org\/original_news\/news16\">DNA Fingerprinting is Everywhere<\/a>&nbsp;-discusses some applications.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.cals.ncsu.edu\/agcomm\/magazine\/spring04\/researcher.htm\">NCSU Researcher Identifies Pathogen Strain Responsible for Irish Potato Famine using DNA Fingerprinting<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/news.ncsu.edu\/2011\/08\/mkgraves\/\">DNA &#8220;Fingerprint&#8221; Can Aid Food Safety<\/a>&nbsp;&#8211; NCSU researcher<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; DNA Fingerprinting &#8211; Online Make Up Module Read along in your lab handout with the information in this webpage.&nbsp; Fill in and answer all questions accordingly in the handout.&nbsp; This handout will be collected and graded later. Lab handout for DNA Fingerprinting &#8211; Online Make Up Module &#8211; this is based on a different [&hellip;]<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2659","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2659","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=2659"}],"version-history":[{"count":19,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2659\/revisions"}],"predecessor-version":[{"id":3772,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2659\/revisions\/3772"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=2659"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}