{"id":2982,"date":"2022-04-01T12:09:54","date_gmt":"2022-04-01T16:09:54","guid":{"rendered":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/?page_id=2982"},"modified":"2023-05-10T22:15:14","modified_gmt":"2023-05-11T02:15:14","slug":"unit-4-labs-2-3-gene-transformation-make-up-module","status":"publish","type":"page","link":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/unit-4-labs-2-3-gene-transformation-make-up-module\/","title":{"rendered":"Unit 4: Labs 2 &amp; 3 Gene Transformation &#8211; Make Up Module"},"content":{"rendered":"\n<div id=\"primary\" class=\"content-area \">\n<article id=\"post-1548\" class=\"post-1548 page type-page status-publish hentry\">\n<div class=\"entry-content\">\n<h2><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/GFP-jellyfish.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-thumbnail wp-image-66\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/05\/GFP-jellyfish-150x150.jpg\" alt=\"Glowing jellyfish\" width=\"150\" height=\"150\"><\/a><\/h2>\n<h2>Gene Transformation \u2013 Online Make Up Module<\/h2>\n<p><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/11\/Gene-TransformationWk1_Fall21_OnlineMakeUp.docx\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">Lab Handout for Gene Transformation lab \u2013 Part 1 Online Make UP Module<\/span><\/a><\/p>\n<h4><span style=\"color: #000000\"><em>Read along in your Part 1 and Part 2 lab handouts with the information on this webpage.&nbsp; Fill in and answer all questions accordingly in your lab handout.&nbsp; These will be collected and graded later.<\/em><\/span><\/h4>\n<h4><span style=\"color: #000000\"><em>Parts 1 and 2 will cover 2 weeks of lab.<\/em><\/span><\/h4>\n<p>&nbsp;<\/p>\n<figure><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-312\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg\" alt=\"pGlo Plasmid DNA\" width=\"225\" height=\"224\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images.jpg 225w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/images-150x150.jpg 150w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/a><\/figure>\n<figure><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-313 size-full\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg\" alt=\"pGlo plasmid and transformed bacterial culture\" width=\"457\" height=\"154\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle.jpg 457w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGLO_Circle-300x101.jpg 300w\" sizes=\"auto, (max-width: 457px) 100vw, 457px\" \/><\/a><\/figure>\n<p>&nbsp;<\/p>\n<h3 class=\"style11\">&nbsp;<\/h3>\n<h3><span style=\"color: #000000\">Students should view the following short videos before coming to lab this week.&nbsp; This will provide good background.&nbsp; Also read the Additional Background Information in your lab handout.<\/span><\/h3>\n<h3><span class=\"style11\"><a href=\"https:\/\/www.youtube.com\/watch?v=_HdMkSEQU8k\" target=\"_blank\" rel=\"noopener\">Stanford Scientists Produce Cancer Drugs from Rare Plants<\/a><span style=\"color: #000000\">(1:59) <span style=\"font-size: 14pt\">by making plasmids and inserting them into more easily grown plants or yeasts. <\/span><\/span><\/span><\/h3>\n<h3><a href=\"https:\/\/www.youtube.com\/watch?v=Ik_Pxht1LM0\">Using Restriction Enzymes and DNA Ligase to make plasmids<\/a>&nbsp;<span style=\"color: #000000\">(1:38)<\/span><\/h3>\n<h3><span class=\"style11\"><a href=\"http:\/\/www.youtube.com\/watch?v=GmhD-RWNL6c&amp;sns=fb\">Funding Basic Science to Revolutionize Medicine<\/a><span style=\"color: #000000\">(3:59) <span style=\"font-size: 14pt\">that show how the focus in research has changed over the past 50 years.<\/span><\/span><\/span><\/h3>\n<h3><a class=\"style11\" href=\"http:\/\/www.youtube.com\/watch?v=Sl2PRHGpYuU&amp;feature=related\">How GFP is used in research<\/a><span class=\"style11\"><span style=\"color: #000000\">(10:03)<\/span><\/span><\/h3>\n<h3><a href=\"https:\/\/www.youtube.com\/watch?v=c40UudFIlGw&amp;t=2s\" target=\"_blank\" rel=\"noopener noreferrer\">Video of BIORAD pGlo Transformation procedure<\/a><span style=\"color: #000000\"> (7:01) <span style=\"font-size: 14pt\">&#8211; what you would have done in lab this week.&nbsp; You may want to follow along in the Activity 2 directions in your lab handout.&nbsp;&nbsp;<\/span><\/span><\/h3>\n<p>&nbsp;<\/p>\n<figure><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-1949\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome-300x141.jpg\" alt=\"\" width=\"300\" height=\"141\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome-300x141.jpg 300w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2020\/10\/Bacterial-chromosome.jpg 320w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000\">Activity 1: In-Lab discussion with your TA \u2013 Important Concepts in the Transformation Procedures:<\/span><\/h3>\n<h3><span class=\"style11\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/pGlo-Plasmid.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">pGlo Plasmid<\/a>&nbsp;<span style=\"color: #000000\">we will use in lab.&nbsp; Why are these genes important?&nbsp; What do they code for?<\/span><\/span><\/h3>\n<h3><span class=\"style11\"><a href=\"http:\/\/dnalc.org\/resources\/animations\/transformation2.html\">DNA transformation using plasmids<\/a>&nbsp;<span style=\"color: #000000\">\u2013 animation explaining the steps in our Transformation procedure.&nbsp; It is very important to understand the nature of the bacterial cell wall, why CaCl<sub>2<\/sub>&nbsp;is used, and why the heat shock is important.<\/span><\/span><\/h3>\n<h3><span style=\"color: #000000\">Answer the Protocol Discussion questions #1-8.<\/span><\/h3>\n<p>&nbsp;<\/p>\n<p><strong>Measurement marks on disposable plastic pipettes provided in the BIORAD kit:<\/strong><\/p>\n<figure><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-314\" src=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg\" alt=\"pipet measurements\" width=\"720\" height=\"540\" srcset=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram.jpg 720w, https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2018\/06\/1-ml-pipet-diagram-300x225.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/a><\/figure>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000\">Activity 3 \u2013 Experimental Treatments and Proposed Results<\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">Fill out the table at the end of this handout to indicate what you think the results would be for each of the 4 agar plates that were inoculated in this procedure.&nbsp;&nbsp;<strong>Also<\/strong>&nbsp;fill out the gray boxes for the agar plates that were not inoculated, but still indicate what you\u2019d expect to happen with those nutrient conditions for the bacterial growth plates that did and did not get the plasmid added.&nbsp; Include why you think you\u2019d get these results in ALL the boxes as well.<\/span><\/li>\n<\/ul>\n<h3><span style=\"color: #000000\">End of Transformation Part 1 (week 1)<\/span><\/h3>\n<\/div>\n<\/article>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\">&nbsp;<\/h3>\n\n\n\n<div id=\"primary\" class=\"content-area \">\n<article id=\"post-1548\" class=\"post-1548 page type-page status-publish hentry\">\n<div class=\"entry-content\">\n<h3>&nbsp;<\/h3>\n<h3><span style=\"color: #000000\">Start of Transformation Part 2 (week 2)<\/span><\/h3>\n<h2>Transformation Efficiency Calculation &#8211; Transformation Lab Part 2 (week 2)<\/h2>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2021\/11\/Transformation-Wk2-Efficiency-Calculations-OnlineMakeUpModule-1.docx\" target=\"_blank\" rel=\"noopener noreferrer\">Lab Handout for Gene Transformation Calculations Online \u2013 Part 2<\/a> \u2013 read before coming to lab.&nbsp;<\/span><\/p>\n<h3><span style=\"color: #000000\">Activity 1: Data Collection from Transformation Plates<\/span><\/h3>\n<h3><span style=\"color: #000000\">Actual Results:&nbsp; The following pictures show the bacterial growth on the different nutrient agar plates under white light and UV light from a previous semester.<\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">Record the data in Table 1 in your lab handout that corresponds to your assigned Group.&nbsp; Be sure to collect data from both the White Light and the UV Light pictures.&nbsp; For example, if you are assigned to Group 3, you only need to record data from the Group 3 pictures &#8211; both white light (3-WL) and UV light (3-UV).<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Answer questions #2-6.<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt\"><strong>Images of bacterial growth from 4 different student lab groups.<\/strong><\/span><\/p>\n<p><span style=\"font-size: 12pt\">\u201c+\u201d Refers to the addition of DNA\/Plasmid, \u201c-\u201d refers to no addition of DNA\/Plasmid to the bacterial samples.&nbsp;&nbsp;<\/span><\/p>\n<table>\n<tbody>\n<tr>\n<td><span style=\"font-size: 12pt\"><strong>Light Source<\/strong><\/span><\/td>\n<td><span style=\"font-size: 12pt\"><strong>Group 1<\/strong><\/span><\/td>\n<td><span style=\"font-size: 12pt\"><strong>Group 2<\/strong><\/span><\/td>\n<td><span style=\"font-size: 12pt\"><strong>Group 3<\/strong><\/span><\/td>\n<td><span style=\"font-size: 12pt\"><strong>Group 4<\/strong><\/span><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 12pt\"><strong>White Light (WL*)<\/strong><\/span><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group1-WL-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">1-WL<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group2-WL-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">2-WL<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group-3-WL-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">3-WL<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group4-WL-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">4-WL<\/span><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<tr>\n<td><span style=\"font-size: 12pt\"><strong>UV Light (UV*)<\/strong><\/span><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group1-UV-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">1-UV<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group2-UV-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">2-UV<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group-3-UV-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">3-UV<\/span><\/a><\/td>\n<td><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/04\/Group-4-UV-M.jpg\" target=\"_blank\" rel=\"noopener noreferrer\"><span style=\"font-size: 12pt\">4-UV<\/span><\/a><\/td>\n<td>&nbsp;<\/td>\n<td>&nbsp;<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h3>* We have pictures for all 6 possible plates both white light UV light, though in the lab students will only get access to 1LB plate, 2 LB\/Amp plates and 1 LB\/Amp\/Ara plate (4 plates total).<\/h3>\n<h3><span style=\"color: #000000\">Activity 2: Calculating Transformation Efficiency<\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 12pt\">Read through the provided information and follow the prompts to calculate the transformation efficiency on your assigned&nbsp;<strong>+LB\/amp\/ara agar plate.<\/strong>&nbsp; Be sure to include the units as you set up each equation.<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Your TA will collect transformation efficiency data from each group so that you can fill out Table 2 Class Results and answer all the Discussion Questions at the end of the lab unit.<\/span><\/li>\n<li>&nbsp;<\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=plVk4NVIUh8&amp;t=14s\" target=\"_blank\" rel=\"noopener noreferrer\">The Evolution of Bacteria on a Mega-Plate Petri Dish<\/a> &#8211; Natural mutation for antibiotic resistance in bacteria growing on nutrient agar containing an antibiotic.<\/span><\/p>\n<h3 class=\"style10\"><span style=\"color: #000000\"><a style=\"color: #000000\" href=\"http:\/\/www.youtube.com\/watch?v=4PKjF7OumYo&amp;feature=related\">Molecular Visualization of DNA&nbsp;<\/a><\/span><\/h3>\n<p class=\"style10\"><span style=\"font-size: 12pt\">0-1:48 = DNA Structure \u2014 Also seen in the Mitosis and Meiosis lab<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">1:48-2:53 = DNA Replication \u2013Can you relate this to DNA synthesis? = S phase that you leaned about in the Mitosis and Meiosis in lecture<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">2:53-4:48 = Transcription \u2013 (you will learn about this and Translation later in lecture)<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">4:48-6:57 = Translation \u2013&nbsp;(you will learn about this and Translation later in lecture)<\/span><\/p>\n<p class=\"style10\"><span style=\"font-size: 12pt\">6:58-7:47 = Hemoglobin and Sickle Cell Anemia&nbsp; -not narrated<\/span><\/p>\n<\/div>\n<p><\/p><\/article><\/div>\n\n\n\n\n\n<div class=\"entry-content\">\n<h3 class=\"style9\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/03\/BIO-183-DNA-Pres-ExpectationsSelf-Assess-Sp2022.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Presentation Expectations and Self Assessment <\/a>\u2013 30 points total<\/h3>\n<\/div>\n\n\n\n<article id=\"post-1548\" class=\"post-1548 page type-page status-publish hentry\">\n<div class=\"entry-content\">\n<h3 class=\"style9\"><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/03\/DNAPresEvalSheetTA_2022.docx\" target=\"_blank\" rel=\"noopener noreferrer\">DNA Presentation Evaluation Sheet<\/a><\/h3>\n<h3><a href=\"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-content\/uploads\/sites\/74\/2022\/10\/Peer-Evaluation-Form-for-DNA-Presentations.docx\" target=\"_blank\" rel=\"noreferrer noopener\">Peer Evaluation form for DNA Group Presentations<\/a>&nbsp;<\/h3>\n<p>&nbsp;<\/p>\n<p><strong>Additional Information on Biotechnology<\/strong>:<\/p>\n<h3 class=\"style11\"><span style=\"color: #000000\">Check out the opportunities for undergraduate students! Certificate, Minor, Courses\u2026..(look under Academic Programs)<\/span><\/h3>\n<h3><span style=\"color: #000000\">Link to information on a Minor in the&nbsp;<\/span><a href=\"https:\/\/biotech.ncsu.edu\/minors-certificate\/\" target=\"_blank\" rel=\"noopener noreferrer\">Biotechnology Program at NCSU<\/a>&nbsp;<span style=\"color: #000000\">and<\/span>&nbsp;<a href=\"https:\/\/biotech.ncsu.edu\/spring-2022\/\" target=\"_blank\" rel=\"noopener noreferrer\">Courses in Biotechnology (BIT) at NCSU<\/a><\/h3>\n<h3 class=\"style11\"><span style=\"color: #000000\">Link to the<\/span>&nbsp;<a href=\"https:\/\/www.btec.ncsu.edu\/academic\/undergraduate\/index.php\" target=\"_blank\" rel=\"noopener noreferrer\">Undergraduate Programs at the BTEC center on Centennial Campus NCSU<\/a>&nbsp;<span style=\"color: #000000\">\u2013 where industry and education meet.<\/span><\/h3>\n<h3 class=\"style11\"><span style=\"color: #000000\">At BTEC they use similar transformation procedures to engineer bacteria and yeasts to make (Biomanufacturing) chemicals, medicines, vaccines, enzymes, proteins, biofuels\u2026\u2026.<\/span><\/h3>\n<h3><span style=\"color: #000000\">Careers in Biotech \u2013<\/span>&nbsp;<a href=\"https:\/\/innovatebio.org\/resource\/biotech-careers?WT.mc_id=200909029014&amp;mkt_tok=eyJpIjoiTkRVd1pqbGlNakJqTlRneSIsInQiOiJGMVViZXFiXC9SeFpRZ1wvV0J3YzBscXRYNzJOdmc0Q3FoeGVMRlJ0dnVOeG92RnI5eVAwSjFsUUZacGVudkZmMmdBV1g2K1JYTk9JZVVxbjJzaHNSN3B6MnhCaStxeDh6bjdaOFBzaENrbTQ2QnpZdXNKSVZSK2hOUmZ2Y0Z5R2x4In0%3D\" target=\"_blank\" rel=\"noopener noreferrer\">nice website.&nbsp;&nbsp;<\/a><span style=\"color: #000000\">Careers&nbsp;at the Bench and Beyond<\/span><\/h3>\n<h3 class=\"style11\"><span style=\"color: #000000\">Great Short articles related to this important technology<\/span><\/h3>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.conncoll.edu\/ccacad\/zimmer\/GFP-ww\/cooluses0.html\" target=\"_blank\" rel=\"noopener noreferrer\">GFP \u2013 Green Fluorescent Protein<\/a>&nbsp;\u2013 Cool Uses<\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.genetics.org\/content\/199\/2\/293\" target=\"_blank\" rel=\"noopener noreferrer\">Brianbow<\/a>&nbsp;\u2013 New Resources and Emerging Biological Applications for Multicolor Genetic Labeling and Analysis \u2013 Informative and beautiful images!<\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Animation<\/a>&nbsp;\u2013 a nice simple step by step animation to show how PCR works.<\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a>&nbsp;\u2013 animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/span><\/p>\n<\/div>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/www.bio-rad.com\/webroot\/web\/movies\/lse\/global\/english\/what-is-polymerase-chain-reaction\/tutorial.html\" target=\"_blank\" rel=\"noopener noreferrer\">PCR Animation<\/a>&nbsp;\u2013 a nice simple step by step animation to show how PCR works.<\/span><\/p>\n<p><span style=\"font-size: 12pt\"><a href=\"https:\/\/biorad-ads.com\/elisa_antibody-animation\/elisa_antibody-test-full.html\" target=\"_blank\" rel=\"noopener noreferrer\">ELISA Anitbody tests<\/a>&nbsp;\u2013 animation that shows the different steps involved in ELISA testing for the presence of antibodies.<\/span><\/p>\n<\/article>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp;<\/p>\n","protected":false},"author":363,"featured_media":0,"parent":0,"menu_order":17,"comment_status":"closed","ping_status":"closed","template":"page-templates\/page-full-width.php","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-2982","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2982","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/users\/363"}],"replies":[{"embeddable":true,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/comments?post=2982"}],"version-history":[{"count":29,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2982\/revisions"}],"predecessor-version":[{"id":3774,"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/pages\/2982\/revisions\/3774"}],"wp:attachment":[{"href":"https:\/\/wordpress-projects.wolfware.ncsu.edu\/bio-183-lab-yhnn8gz\/wp-json\/wp\/v2\/media?parent=2982"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}