Protein Purification and Chromatography

GFP Structure Jellyfish and GFP Protein separation column

Transformed bacteria grown in LB/amp/ara broth under UV light.

Transformed bacteria under UV

 

A Green Light for Biology – Making the Invisible Visible (video) (10:03)

GFP Green Fluorescent Protein – check out some of these cools links – Nobel Prize, uses, techniques, history,…..

GFP Purification Procedure (video, an extension of the Transformation lab) (3:34).

More detailed GFP Purification Procedure- narrated (6:37)

Diagram of protein separation

 

Protein column and rinses

 

Various fluorescent proteins

 

Opportunities at NCSU

 

Bioprocessing – Protein Production and Purification on an Industrial Scale (large volumes!) (19:52) There is a small segment in the middle (about 4:44) that discusses the hydrophillic/hydrophobic nature of the column beads, proteins and salt washes.  Other parts also discuss separation by charge and size using different types of columns.

Large fermentation tanks