Transformed bacteria grown in LB/amp/ara broth under UV light.
A Green Light for Biology – Making the Invisible Visible (video) (10:03)
GFP Green Fluorescent Protein – check out some of these cools links – Nobel Prize, uses, techniques, history,…..
GFP Purification Procedure (video, an extension of the Transformation lab) (3:34).
More detailed GFP Purification Procedure- narrated (6:37)
Opportunities at NCSU
- Minor in Biotechnology at NCSU (BIT Program) and Courses in Biotech (BIT)
- NCSU Professional Sciences Master’s Degree (MMB Program – Master’s of Microbial Biotechnology)
- Biomanufacturing on a larger scale at BTEC (on Centennial Campus)
Bioprocessing – Protein Production and Purification on an Industrial Scale (large volumes!) (19:52) There is a small segment in the middle (about 4:44) that discusses the hydrophillic/hydrophobic nature of the column beads, proteins and salt washes. Other parts also discuss separation by charge and size using different types of columns.