Unit 2: Labs 2-3

 Properties of Enzymes (2 weeks of lab)

 

 Enzyme Proteries  Effect of Inhibitors

How Cells Work – Enzymes

Chemical Reactions and Activation Energy

Graph of Activation Energy

 

Catalase

Catalase structure

 

Animation – Enzyme Function and the difference between competitive and non-competitive inhibitors.

Activity 1:  A Quantitative Study of Catalase Activity

Procedure A: Testing the Activity of Catalase (p. 19-20)

making catalase, boiled, 4 basic tubes

 

Procedure B: Testing the Activity if Catalase Under Six Different Conditions (p. 21-32)

 

Activity 2: A Quantitative Study of Catalase Activity

Procedure C: Using a Spectrophotometer to Monitor Baseline Catalase Activity (p. 33-35)

 

Procedure D: Using a Spectrophotometer to Monitor the Activity of Catalase Under Six Different Environmental Conditions (p. 36-40)

For Activity C & D:

How spectrophotometers work – transmittance and absorbance

How to use a Genesys 10 Spectrophotometer

[callout bodyfontface=”universroman” heading=”Take Home Assignment: Enzyme Lab” headingicon=”noicon” textalign=”textleft” url=”https://wordpress-projects.wolfware.ncsu.edu/bio-183-lab-yhnn8gz/wp-content/uploads/sites/74/2020/02/Enz-30-pt-Unit-Assign-Sp2020.docx” target=”true” type=”basic” bgcolor=”huntyellow”]This assignment will help you review the key components of writing a Methods and Results section, and finding pertinent references for your research. It is worth 30 points and due at the start of lab Mar. 2-6.

Click on this box to download the assignment.[/callout]

Help for Writing the Different Sections of Lab Reports

Finding and Citing References in your Lab Report